Construct: ORF TRCN0000480645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006112.1_s317c1
- Derived from:
- ccsbBroadEn_07608
- DNA Barcode:
- TGAGCCACATTTCAACCATACGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLFM1 (10439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10439 | OLFM1 | olfactomedin 1 | NM_014279.5 | 99.9% | 100% | 183T>C |
| 2 | human | 10439 | OLFM1 | olfactomedin 1 | NM_001282612.1 | 95.5% | 93.8% | (many diffs) |
| 3 | human | 10439 | OLFM1 | olfactomedin 1 | NM_001282611.2 | 92.4% | 91.1% | (many diffs) |
| 4 | human | 10439 | OLFM1 | olfactomedin 1 | NM_006334.4 | 28.8% | 28.6% | 183T>C;403_404insCGATAAAA;405_406ins988 |
| 5 | mouse | 56177 | Olfm1 | olfactomedin 1 | NM_001038613.1 | 89% | 95.7% | (many diffs) |
| 6 | mouse | 56177 | Olfm1 | olfactomedin 1 | NM_019498.2 | 82.7% | 87.8% | (many diffs) |
| 7 | mouse | 56177 | Olfm1 | olfactomedin 1 | NM_001038614.1 | 24.8% | 25.4% | (many diffs) |
| 8 | mouse | 56177 | Olfm1 | olfactomedin 1 | NM_001038612.1 | 22.5% | 21.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1467
- ORF length:
- 1401
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aggtcgttgg aggtggcagc gagacatgca cccggcccgg aagctcctca 121 gcctcctctt cctcatcctg atgggcactg aactcactca agtgctgccc accaaccctg 181 aggagagctg gcaggtgtac agctctgccc aggacagcga gggcaggtgt atctgcacag 241 tggtcgcccc acagcagacc atgtgttcac gggatgcccg cacaaaacag ctgaggcagc 301 tactggagaa ggtgcagaac atgtctcaat ccatagaggt cttggacagg cggacccaga 361 gagacttgca gtacgtggag aagatggaga accaaatgaa aggactggag tccaagttca 421 aacaggtgga ggagagtcat aagcaacacc tggccaggca gtttaaggcg ataaaagcga 481 aaatggatga acttaggcct ttgatacctg tgttggaaga gtacaaggcc gatgccaaat 541 tggtattgca gtttaaagag gaggtccaga atctgacgtc agtgcttaac gagctgcaag 601 aggaaattgg cgcctatgac tacgatgaac ttcagagcag agtgtccaat cttgaagaaa 661 ggctccgtgc atgcatgcaa aaactagctt gcgggaagtt gacgggcatc agtgaccccg 721 tgactgtcaa gacctccggc tcgaggttcg gatcctggat gacagaccct ctcgcccctg 781 aaggcgataa ccgggtgtgg tacatggacg gctatcacaa caaccgcttc gtacgtgagt 841 acaagtccat ggttgacttc atgaacacgg acaatttcac ctcccaccgt ctcccccacc 901 cctggtcggg cacggggcag gtggtctaca acggttctat ctacttcaac aagttccaga 961 gccacatcat catcaggttt gacctgaaga cagagaccat cctcaagacc cgcagcctgg 1021 actatgccgg ttacaacaac atgtaccact acgcctgggg tggccacTCG GACATCGACC 1081 TCATGGTGGA CGAGAGCGGG CTGTGGGCCG TGTACGCCAC CAACCAGAAC GCTGGCAACA 1141 TCGTGGTCAG TAGGCTGGAC CCCGTGTCCC TGCAGACCCT GCAGACCTGG AACACGAGCT 1201 ACCCCAAGCG CAGCGCCGGG GAGGCCTTCA TCATCTGCGG CACGCTGTAC GTCACCAACG 1261 GCTACTCAGG GGGTACCAAG GTCCACTATG CATACCAGAC CAATGCCTCC ACCTATGAAT 1321 ACATCGACAT CCCATTCCAG AACAAATACT CCCACATCTC CATGCTGGAC TACAACCCCA 1381 AGGACCGGGC CCTGTATGCC TGGAACAACG GCCACCAGAT CCTCTACAAC GTGACCCTCT 1441 TCCACGTCAT CCGCTCCGAC GAGTTGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGAGCCAC 1621 ATTTCAACCA TACGGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt