Construct: ORF TRCN0000480681

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002968.4_s317c1
Derived from:
ccsbBroadEn_14934
DNA Barcode:
TACACAATAACACTCCCGACAATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS6KA5 (9252)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480681
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_182398.2 100% 100%
2 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322229.2 69% 68.9% 1645_1648delAATTinsG;1651_2385del
3 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_004755.4 68.4% 68.3% 1645_1648delAATTinsG;1651_2406del
4 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322236.2 64.9% 64.8% 618_619ins84;1561_1564delAATTinsG;1567_2322del
5 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322228.2 63.9% 63.8% 510_511ins108;1537_1540delAATTinsG;1543_2298del
6 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322233.2 61.6% 61.5% 951_952ins162;1483_1486delAATTinsG;1489_2244del
7 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322232.2 59.3% 59.2% 173_174ins219;1426_1429delAATTinsG;1432_2187del
8 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322235.2 58.5% 58.4% 0_1ins237;1408_1411delAATTinsG;1414_2169del
9 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322237.2 58.5% 58.4% 0_1ins237;1408_1411delAATTinsG;1414_2169del
10 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322234.2 44.1% 43.3% (many diffs)
11 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322238.2 41.1% 41% 0_1ins657;988_991delAATTinsG;994_1749del
12 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 XM_017021786.2 41.1% 41% 0_1ins657;988_991delAATTinsG;994_1749del
13 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322231.2 38.6% 35.3% (many diffs)
14 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322230.2 35% 34.9% (many diffs)
15 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322227.2 33.3% 33.2% 0_1ins843;802_805delAATTinsG;808_1563del
16 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... NM_001330702.1 62.1% 66.5% (many diffs)
17 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... XM_006516275.3 59.5% 63.5% (many diffs)
18 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... NM_153587.3 57.4% 61.5% (many diffs)
19 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... XM_006516276.3 37.1% 39.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1713
ORF length:
1647
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggaggagggt ggcagcagcg gcggcgccgc ggggaccagc gcggacggcg
121gcgacggagg agagcagctc ctcactgtca agcacgagct gcggactgct aatttgacag
181gacatgctga gaaggtggga atagaaaatt ttgagctcct gaaggtccta ggaactggag
241cttatggaaa agtatttcta gttcgtaaaa taagtggcca tgatactgga aagctgtatg
301ccatgaaagt tttgaaaaag gcaacaatcg ttcaaaaggc caaaaccaca gagcatacaa
361ggacagaacg acaagtcctg gaacacatta ggcagtcgcc atttttggta acattacatt
421atgctttcca gacagaaacc aaacttcatc tcattttaga ttatataaat ggtggtgaac
481tttttactca tctttctcaa agagagcgtt tcacagagca tgaggtgcag atttatgttg
541gagagattgt gcttgccctc gaacatctcc acaagttggg gattatatat cgtgatatta
601agcttgagaa tattctactt gattctaatg gccatgtggt gctgacagat tttggtctga
661gtaaggagtt tgtggctgat gaaactgaaa gagcatattc cttttgtgga actattgaat
721acatggcacc agatattgtc agagggggag attcaggaca tgacaaggca gttgactggt
781ggagtttggg tgttctaatg tatgaattac taactggagc atctcctttc actgttgatg
841gagaaaaaaa ttcccaagct gagatatcta ggagaatatt aaaaagtgag cctccatatc
901cccaagaaat gagtgcttta gcgaaagacc taattcagcg tcttttgatg aaagatccca
961agaagagatt gggatgtggt ccacgtgatg cagatgaaat caaagaacat ctcttctttc
1021agaaaataaa ttgggatgat ttagccgcca aaaaagtgcc tgcaccattt aagccagtca
1081ttcgagatga attagatgtg agtaactttg cagaagagtt cacagaaatg gatcccactt
1141attctcccgc agccctgccc cagagttctg agaagctgtt tcagggctat tcctttgttg
1201ctccttccat cctattcaag cgtaatgcag ctgtcataga ccctcttcag tttcacatgg
1261gagttgaacg tcctGGAGTG ACAAATGTTG CCAGGAGTGC AATGATGAAG GACTCTCCAT
1321TCTATCAACA CTATGACCTA GATTTGAAGG ACAAACCCCT GGGAGAAGGT AGTTTTTCAA
1381TTTGTCGAAA GTGTGTGCAT AAAAAAAGTA ACCAAGCTTT TGCAGTCAAA ATAATCAGCA
1441AAAGGATGGA AGCCAATACT CAAAAGGAAA TAACAGCTCT GAAACTCTGT GAAGGACACC
1501CCAATATTGT GAAGTTGCAT GAAGTTTTTC ATGATCAGCT TCACACGTTT CTAGTGATGG
1561AACTTCTGAA TGGAGGAGAA CTGTTTGAGC GCATTAAGAA AAAGAAGCAC TTCAGTGAGA
1621CGGAAGCCAG CTACATCATG AGGAAGCTTG TTTCAGCTGT AAGCCACATG CATGATGTTG
1681GAGTGGTGCA CAGGGATCTG AAACCTGAGG TATACCCAAC TTTCTTGTAC AAAGTGGTTG
1741ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1801GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA
1861CACAATAACA CTCCCGACAA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat
1921ttgtgaaaga tt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.