Construct: ORF TRCN0000480767
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011143.2_s317c1
- Derived from:
- ccsbBroadEn_13042
- DNA Barcode:
- GCTGGCAATCCTATTCCCTAGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSBPL11 (114885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480767
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114885 | OSBPL11 | oxysterol binding protein l... | NM_022776.5 | 49.1% | 49.1% | 1_1140del |
| 2 | human | 114885 | OSBPL11 | oxysterol binding protein l... | XR_001739992.2 | 25.8% | (many diffs) | |
| 3 | mouse | 106326 | Osbpl11 | oxysterol binding protein-l... | XM_011245797.2 | 46.1% | 49.1% | (many diffs) |
| 4 | mouse | 106326 | Osbpl11 | oxysterol binding protein-l... | NM_176840.3 | 42% | 44.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttaacaaga gtggtgcttc ctacatttat cctagagaag cgttccttgc 121 tggaaatgta tgcagacttt atgtctcatc cagacctatt tatagccatc actaatggag 181 ccacagctga ggacagaatg attcgctttg ttgagtacta ccttacctca tttcatgaag 241 gccgtaaggg agccattgct aaaaaaccat acaatcctat cattggagaa acatttcact 301 gttcctggaa gatgccaaaa agcgaggtag catccagtgt ttttagcagt tcttccaccc 361 agggagtcac aaatcatgct cctttatcgg gggagtcttt gacccaggtg ggatcagact 421 gttacacagt cagatttgtt gctgagcagg tttctcatca tcctccagtc tcaggatttt 481 atgcagaatg tacagagagg aagatgtgtg taaatgcgca tgtctggact aagagcaagt 541 tcttaggcat gtcaataggc gtgacaatgg ttggagaagg tatccttagt ctgttggagc 601 atggagaaga gtacacattt tctctaccct gtgcatatgc TCGGTCAATT TTGACTGTTC 661 CTTGGGTAGA ACTGGGTGGC AAAGTCAGTG TCAACTGTGC AAAAACTGGA TATTCAGCCA 721 GCATCACTTT TCATACCAAG CCATTTTATG GTGGCAAACT GCATCGGGTT ACAGCTGAAG 781 TAAAGCACAA CATCACCAAC ACTGTGGTAT GCAGAGTGCA AGGGGAATGG AATAGTGTTC 841 TTGAGTTCAC ATATAGCAAT GGAGAGACAA AGTATGTGGA CTTGACTAAA TTGGCAGTGA 901 CGAAGAAAAG AGTGAGACCT CTGGAGAAGC AGGATCCATT TGAATCCAGG CGATTGTGGA 961 AAAATGTGAC AGACTCGCTG AGAGAATCTG AAATTGATAA GGCCACAGAG CATAAGCATA 1021 CCCTGGAAGA ACGTCAGAGG ACTGAAGAAA GGCATCGTAC TGAAACAGGC ACACCTTGGA 1081 AAACCAAATA TTTTATTAAA GAGGGAGATG GCTGGGTTTA TCATAAACCA CTTTGGAAAA 1141 TAATTCCAAC AACACAACCA GCAGAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCTGGCAA 1321 TCCTATTCCC TAGAACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt