Construct: ORF TRCN0000480837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015561.1_s317c1
- Derived from:
- ccsbBroadEn_13336
- DNA Barcode:
- CTCGATCCCTCTGATTCGAACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND1B (163486)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163486 | DENND1B | DENN domain containing 1B | NM_001300858.2 | 99.9% | 99.7% | 929C>A |
| 2 | human | 163486 | DENND1B | DENN domain containing 1B | NM_144977.5 | 90.7% | 86.9% | (many diffs) |
| 3 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509251.3 | 74.5% | 69.3% | (many diffs) |
| 4 | human | 163486 | DENND1B | DENN domain containing 1B | XM_017000471.1 | 52.6% | 51.2% | (many diffs) |
| 5 | human | 163486 | DENND1B | DENN domain containing 1B | XM_006711192.2 | 51.2% | 49.8% | (many diffs) |
| 6 | human | 163486 | DENND1B | DENN domain containing 1B | XM_006711193.3 | 51.2% | 49.8% | (many diffs) |
| 7 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509249.2 | 51.2% | 49.8% | (many diffs) |
| 8 | human | 163486 | DENND1B | DENN domain containing 1B | XM_017000470.1 | 51.2% | 49.8% | (many diffs) |
| 9 | human | 163486 | DENND1B | DENN domain containing 1B | XM_005244931.2 | 50.9% | 47.7% | (many diffs) |
| 10 | human | 163486 | DENND1B | DENN domain containing 1B | NM_001195215.2 | 49.6% | 46.5% | (many diffs) |
| 11 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509248.2 | 49.2% | 45.8% | (many diffs) |
| 12 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509247.1 | 47.8% | 44.9% | (many diffs) |
| 13 | human | 163486 | DENND1B | DENN domain containing 1B | XM_011509246.2 | 46.6% | 43.9% | (many diffs) |
| 14 | human | 163486 | DENND1B | DENN domain containing 1B | XM_024453626.1 | 29.7% | 28.4% | (many diffs) |
| 15 | human | 163486 | DENND1B | DENN domain containing 1B | XM_024453627.1 | 18.5% | 17.3% | (many diffs) |
| 16 | human | 163486 | DENND1B | DENN domain containing 1B | XM_006711194.3 | 15.5% | 14.2% | (many diffs) |
| 17 | human | 163486 | DENND1B | DENN domain containing 1B | NR_125340.2 | 13.5% | (many diffs) | |
| 18 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | NM_001166501.1 | 45.2% | 45.3% | (many diffs) |
| 19 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529716.3 | 44% | 44.2% | (many diffs) |
| 20 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529717.2 | 42% | 43% | (many diffs) |
| 21 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | NM_181347.3 | 38.7% | 39.8% | (many diffs) |
| 22 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529719.2 | 38.3% | 37.3% | (many diffs) |
| 23 | mouse | 329260 | Dennd1b | DENN/MADD domain containing 1B | XM_006529718.2 | 37.3% | 36.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagctcca cgggaggaga aaagatggcc ccaacctgta ttttcgaatc 121 ctgtggtatt gtggaaattc ccagaggact ttggagacca ggaaatacta cagagtgtgc 181 caaagttctg ttttcccttt gacgttgaaa gggtgtctca gaatcaagtt ggacagcact 241 ttacctttgt actgacagac attgaaagta aacagagatt tggattctgc agactgacgt 301 caggaggcac aatttgttta tgcatcctta gttaccttcc ctggtttgaa gtgtattaca 361 agcttctaaa tactcttgca gattacttgg ctaaggaact ggaaaatgat ttgaatgaaa 421 ctctcagatc actgtataac cacccagtac caaaggcaaa tactcctgta aatttgagtg 481 tgcattccta cttcattgcc cctgatgtaa ctggactccc aacaataccc gagagtagaa 541 atcttacaga atattttgtt gccgtggatg tgaacaacat gctgcagctg tatgccagta 601 tgctgcatga aaggcgcatc gtgattatct cgagcaaatt aagcacttta actgcctgta 661 tccatggatc agctgctctt ctatacccaa tgtattggca acacatatac atcccagtgc 721 ttcctccaca cctgctggac tactgctgtg ccccaatgcc atacctgatt ggaatacact 781 ccagccTCAT AGAGAGAGTG AAAAACAAAT CATTGGAAGA TGTTGTTATG TTAAATGTTG 841 ATACAAACAC ATTAGAATCA CCATTTAGTG ACTTGAACAA CCTACCAAGT GATGTGGTCT 901 CGGCCTTGAA AAATAAACTG AAGAAGCAGT CTACAGCTAC GGGTGATGGA GTAGCTAGGG 961 CCTTTCTTAG AGCACAGGCT GCTTTGTTTG GATACTACAG AGATGCACTG AGATACAAAC 1021 CTGGTGAGCC CATCACTTTC TGTGAGGAGA GTTTTGTAAA GCACCGCTCA AGCGTGATGA 1081 AACAGTTCCT GGAAACTGCC ATTAACCTCC AGCTTTTTAA GCAGTTTATC GATGGTCGAC 1141 TGGCAAAACT AAATGCAGGA AGGGGTTTCT CTGATGTATT TGAAGAAGAG ATCACTTCAG 1201 GTGGCTTTTG TGGAGGTAAA GACAAGTTAC AATATGATTA TCCATTTTCT CAATACCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAC TCGATCCCTC TGATTCGAAC TACACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att