Construct: ORF TRCN0000480894
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010146.1_s317c1
- Derived from:
- ccsbBroadEn_12020
- DNA Barcode:
- TTGCCTACGGTTGCTGGAAAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TM6SF1 (53346)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480894
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001330385.3 | 100% | 100% | |
| 2 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022329.2 | 100% | 100% | |
| 3 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022330.1 | 100% | 100% | |
| 4 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022331.2 | 100% | 100% | |
| 5 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_931848.2 | 73.7% | 1_94del;575_679del;764_765delTG | |
| 6 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022322.1 | 60.2% | 53.3% | 480_799del;885_936del |
| 7 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_001751320.1 | 60.1% | 1_94del;659_937del | |
| 8 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001144903.3 | 55.4% | 49.1% | 481_707del;792_1017del |
| 9 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001353878.2 | 52.2% | 46.2% | 480_799del;885_1080del |
| 10 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022321.1 | 52.2% | 46.2% | 481_707del;792_1080del |
| 11 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_023003.5 | 50.8% | 45% | 480_799del;885_1110del |
| 12 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_011521679.2 | 50.4% | 44.6% | 480_799del;885_1119del |
| 13 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XM_017022320.1 | 48% | 42.6% | 480_799del;885_1173del |
| 14 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_001751318.1 | 47.5% | 1_34del;514_833del;919_1186del | |
| 15 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NR_148560.2 | 31.9% | 1_36del;516_713del;799_1763del | |
| 16 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NR_148561.2 | 31.4% | (many diffs) | |
| 17 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001353880.2 | 28.6% | 22.5% | 0_1ins273;208_434del;519_744del |
| 18 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | NM_001353879.2 | 26.2% | 20.6% | 0_1ins273;207_526del;612_837del |
| 19 | human | 53346 | TM6SF1 | transmembrane 6 superfamily... | XR_001751319.2 | 17.5% | (many diffs) | |
| 20 | mouse | 107769 | Tm6sf1 | transmembrane 6 superfamily... | NM_145375.4 | 45.4% | 43.4% | (many diffs) |
| 21 | mouse | 107769 | Tm6sf1 | transmembrane 6 superfamily... | NR_111910.1 | 24.2% | (many diffs) | |
| 22 | mouse | 107769 | Tm6sf1 | transmembrane 6 superfamily... | NM_001291282.1 | 23.5% | 19.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgcctctgcg gccaccgggg tcttcgtgct gtccctctcg gccatcccgg 121 tcacctatgt cttcaaccac ctggcggccc agcatgattc ctggactatt gtaggggttg 181 ctgccctcat cctgttcctg gtagcactgc tggctcgtgt cctcgtcaaa agaaaaccac 241 cccgggaccc actgttctat gtgtatgcag tttttGGATT TACCAGCGTG GTGAACCTCA 301 TCATAGGACT GGAGCAAGAT GGAATCATTG ACGGGTTCAT GACACACTAC TTGAGAGAGG 361 GTGAACCGTA TCTGAACACC GCATATGGGC ACATGATCTG CTACTGGGAT GGCTCTGCTC 421 ATTATCTGAT GTACCTGGTG ATGGTGGCAG CCATAGCATG GGAGGAAACT TATAGAACCA 481 TTGGCCTATA TTGGGTTGGA TCTATTATTA TGAGTGTTGT TGTTTTTGTG CCAGGAAACA 541 TTGTAGATGC TGGCATATAT GTTCTATTCT GTTCCTTACT TTGTGACTGC ACTGTATGGC 601 TTAGTGGTTC CTGGATGTTC CTGGATGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTGCC 781 TACGGTTGCT GGAAAACCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt