Construct: ORF TRCN0000480915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001940.1_s317c1
- Derived from:
- ccsbBroadEn_11480
- DNA Barcode:
- TTGACTCAACGCGCCATCTCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK4 (10298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014834.3 | 89.6% | 84.7% | (many diffs) |
| 2 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014835.2 | 89.6% | 84.7% | (many diffs) |
| 3 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526320.1 | 89.6% | 84.7% | (many diffs) |
| 4 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_024451314.1 | 89.6% | 84.7% | (many diffs) |
| 5 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014831.3 | 72% | 71.9% | 357_528del;532_854del |
| 6 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014832.2 | 72% | 71.9% | 357_528del;532_854del |
| 7 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_005884.4 | 72% | 71.9% | 357_528del;532_854del |
| 8 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526316.1 | 72% | 71.9% | 357_528del;532_854del |
| 9 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526317.2 | 72% | 71.9% | 357_528del;532_854del |
| 10 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526318.2 | 72% | 71.9% | 357_528del;532_854del |
| 11 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526319.2 | 72% | 71.9% | 357_528del;532_854del |
| 12 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | NM_027470.3 | 63.9% | 70.1% | (many diffs) |
| 13 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312296.1 | 63.9% | 70.1% | (many diffs) |
| 14 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312297.1 | 63.9% | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgggaagagg aagaagcggg tggagatctc cgcgccgtcc aacttcgagc 121 accgcgtgca cacgggcttc gaccagcacg agcagaagtt cacggggctg ccccgccagt 181 ggcagagcct gatcgaggag tcggctcgcc ggcccaagcc cctcgtcgac cccgcctgca 241 tcacctccat ccagcccggg gcccccaaga ccatcgtgcg gggcagcaaa ggtgccaaag 301 atggggccct cacgctgctg ctggacgagt ttgagaacat gtcggtgaca cgctccaact 361 ccctgcggag agacagcccg ccgccgcccg cccgtgcccg ccaggaaaat gggatgccag 421 aaaagccccc tggcccccgc tcaccacagc gggagccaca gcgagtatcc catgagcagt 481 tccgggctgc cctgcagctg gtggtggacc caggcgaccc ccgctcctac ctggacaact 541 tcatcaagat tggcgagggc tccacgggca tcgtgtgcat cgccaccgtg cgcagctcgg 601 gcaagctggt ggccgtcaag aagatggacc tgcgcaagca gcagaggcgc gagctgctct 661 tcaacgaggt ggtaatcatg agggactacc agcacgagaa tgtggtggag atgtacaaca 721 gctacctggt gggggacgag ctctgggtgg tcatggagtt cctggaagga ggcgccctca 781 ccgacatcgt cacccacacc aggatgaacg aggagcagat cgcggccgtg tgccttgcag 841 tgctgcaggc cctgtcggtg ctccacgccc agggcgtcat ccaccgggac atcaagagcg 901 actcgatcct gctgacccat gatggcaggg tgaagctgtc agactttggg ttctgcgccc 961 aggtgagcaa ggaagtgccc cgaaggaagt cgctggtcgg cacgcccTAC TGGATGGCCC 1021 CAGAGCTCAT CTCCCGCCTT CCCTACGGGC CAGAGGTAGA CATCTGGTCG CTGGGGATAA 1081 TGGTGATTGA GATGGTGGAC GGAGAGCCCC CCTACTTCAA CGAGCCACCC CTCAAAGCCA 1141 TGAAGATGAT TCGGGACAAC CTGCCACCCC GACTGAAGAA CCTGCACAAG GTGTCGCCAT 1201 CCCTGAAGGG CTTCCTGGAC CGCCTGCTGG TGCGAGACCC TGCCCAGCGG GCCACGGCAG 1261 CCGAGCTGCT GAAGCACCCA TTCCTGGCCA AGGCAGGGCC GCCTGCCAGC ATCGTGCCCC 1321 TCATGCGCCA GAACCGCACC AGATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGACTCAACG 1501 CGCCATCTCG TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att