Construct: ORF TRCN0000480950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002316.1_s317c1
- Derived from:
- ccsbBroadEn_07487
- DNA Barcode:
- GACCCCTCCATCATAGCGCCCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMS1 (9790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480950
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | NM_014753.4 | 99.9% | 99.9% | 336T>C;2445C>T;3421G>A |
| 2 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XM_005271846.3 | 99.9% | 99.9% | 336T>C;2445C>T;3421G>A |
| 3 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XM_011540402.2 | 99.9% | 99.9% | 336T>C;2445C>T;3421G>A |
| 4 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XM_005271848.3 | 95% | 95% | (many diffs) |
| 5 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XR_002957049.1 | 89.7% | (many diffs) | |
| 6 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XR_001747266.1 | 87.7% | (many diffs) | |
| 7 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XR_246522.1 | 87% | (many diffs) | |
| 8 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XR_428728.2 | 68.9% | (many diffs) | |
| 9 | human | 9790 | BMS1 | BMS1 ribosome biogenesis fa... | XM_011540403.2 | 67.8% | 67.8% | 0_1ins1233;1212C>T;2188G>A |
| 10 | human | 101929959 | LOC101929959 | ribosome biogenesis protein... | XR_002956837.1 | 33.3% | (many diffs) | |
| 11 | human | 101929959 | LOC101929959 | ribosome biogenesis protein... | XM_017015376.1 | 20.5% | 17.1% | (many diffs) |
| 12 | human | 101929959 | LOC101929959 | ribosome biogenesis protein... | XM_017015377.1 | 20.5% | 17.1% | (many diffs) |
| 13 | human | 728034 | BMS1P14 | BMS1 pseudogene 14 | NR_146106.1 | 18.9% | (many diffs) | |
| 14 | human | 728034 | BMS1P14 | BMS1 pseudogene 14 | NR_146108.1 | 17.1% | (many diffs) | |
| 15 | human | 728034 | BMS1P14 | BMS1 pseudogene 14 | NR_146105.1 | 16.6% | (many diffs) | |
| 16 | human | 728034 | BMS1P14 | BMS1 pseudogene 14 | NR_146109.1 | 16.3% | (many diffs) | |
| 17 | human | 728034 | BMS1P14 | BMS1 pseudogene 14 | NR_146110.1 | 15.8% | (many diffs) | |
| 18 | human | 728034 | BMS1P14 | BMS1 pseudogene 14 | NR_146107.1 | 15.3% | (many diffs) | |
| 19 | human | 101929959 | LOC101929959 | ribosome biogenesis protein... | XM_017015378.1 | 14.9% | 10.1% | (many diffs) |
| 20 | human | 101929959 | LOC101929959 | ribosome biogenesis protein... | XM_017015379.1 | 14.2% | 9.8% | (many diffs) |
| 21 | human | 101929959 | LOC101929959 | ribosome biogenesis protein... | XM_011518094.2 | 11.4% | 11.1% | (many diffs) |
| 22 | mouse | 213895 | Bms1 | BMS1, ribosome biogenesis f... | NM_194339.1 | 83.6% | 86.2% | (many diffs) |
| 23 | mouse | 213895 | Bms1 | BMS1, ribosome biogenesis f... | XM_017321497.1 | 82% | 84.4% | (many diffs) |
| 24 | mouse | 213895 | Bms1 | BMS1, ribosome biogenesis f... | XR_377435.3 | 65.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3915
- ORF length:
- 3846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggctaag gaccagaaga aacacagaaa gaaaaacagt ggacccaaag 121 ctgcaaagaa aaagaagcgg cttctgcagg atctccagct aggagacgaa gaagatgccc 181 ggaagagaaa tcccaaagct tttgcagttc agtctgctgt gcggatggct cgatcctttc 241 acaggactca ggatttgaag acaaaaaagc atcatattcc agtggttgat cgaactccac 301 tagagccccc accaatagtg gtagtggtga tgggacctcc aaaagttgga aagagcactt 361 tgatacaatg cctcattcgg aactttaccc ggcagaagtt gaccgagatc agaggccctg 421 tgacgattgt gtcaggtaaa aagcgcagac tcaccattat tgaatgtggg tgtgacatta 481 acatgatgat tgatctggct aaagtagcag atctggtact gatgcttata gatgccagct 541 ttgggtttga aatggaaacg tttgagtttc taaacatctg tcaagtacat ggctttccta 601 aaattatggg agttctcacc cacctcgact ccttcaagca taataagcaa ctgaagaaga 661 caaagaagcg attaaaacac aggttctgga cggaagttta cccgggtgcc aagctgttct 721 acctttctgg aatggtgcat ggagaatatc aaaaccaaga aatccacaat ctgggccgtt 781 ttattacagt tatgaagttt aggcctctca catggcaaac ttctcaccct tatatcctgg 841 cagacaggat ggaagatttg acaaacccag aggatatccg aacaaacatc aaatgtgacc 901 ggaaggtgtc actttatggt tatttaagag gagcacactt gaaaaataaa agccaaattc 961 acatgccagg ggtaggagat tttgccgtga gtgacatcag tttcctccca gacccttgcg 1021 ctcttcctga acaacaaaag aagcgctgtt taaatgagaa ggagaagctg gtttatgcgc 1081 ctctttctgg agttgggggt gtgctgtatg acaaagacgc tgtctatgtt gaccttggtg 1141 gcagccacgt ttttcaggat gaagtggggc ccacccatga gctggtccag agtctcatct 1201 ctacccactc caccattgat gccaagatgg cttcaagtcg agtgacgctg ttttctgatt 1261 ccaagccact tgggtcagag gatatagata atcaagggct aatgatgcca aaggaggaaa 1321 aacaaatgga cttgaacact ggtcgaatgc gtcggaaagc cattttcgga gatgaagatg 1381 aatctggaga tagtgatgat gaagaagatg atgaaatgtc tgaagatgac gggttggaaa 1441 acggctctag tgatgaggaa gcagaagagg aggaaaatgc tgagatgact gatcagtata 1501 tggctgttaa gggcatcaaa cgacggaaac ttgagttgga agaagacagt gaaatggatt 1561 tgccagcatt tgctgacagt gacgatgacc ttgagaggag ctcagcggaa gaaggggaag 1621 cggaggaagc tgatgaaagc agtgaagaag aggactgcac tgcaggagag aagggcattt 1681 caggatcaaa ggctgctgga gaaggtagta aagcagggct gtcaccagct aattgccaga 1741 gtgaccgtgt gaatctggag aagtctttgc tgatgaagaa agcagctctc cccactttcg 1801 attctgggca ttgcacagct gaagaggtgt ttgcatctga agatgaatct gaagaaagct 1861 cctcactcag tgcagaggaa gaagactcag aaaatgaaga ggctattaga aaaaagcttt 1921 caaagccttc tcaagtgagc agtggtcaga aactggggcc acagaacttc attgatgaga 1981 ccagtgatat agaaaattta ctcaaagagg aagaagatta caaggaagaa aataatgatt 2041 ccaaagaaac gtcaggtgcc ctcaagtgga aggaagacct ttccagaaag gcagctgagg 2101 cctttctgag gcagcagcaa gcagctccaa acctccgaaa gcttatttat gggacagtga 2161 cagaagataa tgaagaagaa gatgatgata ctctagaaga gcttggaggg ttgtttcgtg 2221 tcaaccagcc tgacagagag tgtaagcaca aggctgactc tttggactgc tccagatttc 2281 ttgtggaggc cccccatgac tgggatttag aggaggttat gaacagtatc agagattgct 2341 tcgtgactgg aaagtgggaa gatgataaag atgcagccaa ggtcttagca gaagatgagg 2401 agctctacgg tgactttgaa gacttggaaa caggggacgt gcacaaggga aaatcaggcc 2461 ccaatactca gaatgaagat atagagaaag aagttaagga agaaattgac cctgacgaag 2521 aagaaagtgc caagaaaaag catttggata agaagagaaa attgaaggag atgtttgatg 2581 cagaatatga tgaaggagaa agcacatatt ttgatgatct taaaggagaa atgcagaaac 2641 aagcacagct gaatcgcgca gaatttgaag atcaagatga tgaagccaga gttcagtatg 2701 agggttttcg acctgggatg tacgtccgca ttgagattga aaatgttccc tgtgaatttg 2761 tgcagaactt tgacccccat taccccatta tcctgggtgg cttgggcaac agtgagggaa 2821 atgttggcta cgtgcagatg cgtctgaaga aacatcgctg gtataagaaa atcctcaagt 2881 cccgagatcc aatcatattt tctgtagggt ggaggaggtt tcagaccatc ccactgtatt 2941 atatcgaaga ccacaatgga agacaaaggc ttctaaagta taccccacag cacatgcatt 3001 gcggagcagc cttttggggc cctatcactc cacagggaac tggtttcttg gcaatacagt 3061 ctgtcagtgg cataatgcct gattttcgga tagctgctac aggagttgtc cttgatctgg 3121 ataaatccat aaaaattgtg aagaaattaa agctaactgg ttttccatat aaaattttca 3181 agaacacttc atttattaag ggaatgttta attctgcctt ggaagtggcc aaatttgaag 3241 gtgctgtgat tcgaacagtc agtgggataa gggggcagat caagaaagca ctccgagctc 3301 cagaaggagc tttcagggcc agctttgagg ataagctgct gatgagcgat attgtcttca 3361 tgcgaacttg gtatcctgtt tccatcccag cgttctataa cccagtaaca tctttgttga 3421 aaccagtggg tgagaaagac acctggtcag gaatgcggac cacgggccaa ctcaggctcg 3481 cccatggcat cagactaaag gcgaacaagg actctctgta taagccaatc cTGAGGCAAA 3541 AGAAACATTT TAATTCACTG CACATTCCAA AAGCCTTGCA GAAGGCCCTG CCATTTAAGA 3601 ACAAGCCCAA GACCCAAGCA AAGGCAGGCA AGGTGCCAAA GGACAGGCGG AGACCGGCCG 3661 TCATACGCGA GCCTCATGAA AGAAAGATCC TTGCACTGCT GGATGCTCTG AGTACGGTGC 3721 ATAGTCAGAA GATGAAGAAG GCCAAGGAGC AGCGGCACCT GCACAATAAA GAGCACTTCA 3781 GAGCCAAGCA GAAGGAGGAG GAGGAGAAGC TGAAGCGGCA GAAGGACCTC AGGAAGAAGC 3841 TCTTCAGAAT TCAGGGGCAG AAGGAAAGAA GAAACCAGAA GTCCAGTTTG AAGGGGGCTG 3901 AGGGCCAATT GCAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 4021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACCCCTCCA TCATAGCGCC 4081 CGAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt