Construct: ORF TRCN0000480966
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015777.5_s317c1
- Derived from:
- ccsbBroadEn_14561
- DNA Barcode:
- CAAAGATTTTAGCGCCCGCTTTCG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK4 (814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480966
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323374.2 | 99.8% | 99.3% | 1011G>C;1411delC |
| 2 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323375.2 | 99.8% | 99.3% | 1011G>C;1411delC |
| 3 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001744.6 | 99.8% | 99.3% | 1011G>C;1411delC |
| 4 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323376.2 | 58.2% | 57.7% | 0_1ins591;420G>C;820delC |
| 5 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323377.2 | 58.2% | 57.7% | 0_1ins591;420G>C;820delC |
| 6 | human | 814 | CAMK4 | calcium/calmodulin dependen... | XR_001742282.2 | 25.4% | (many diffs) | |
| 7 | human | 814 | CAMK4 | calcium/calmodulin dependen... | XR_001742281.2 | 24.9% | (many diffs) | |
| 8 | human | 814 | CAMK4 | calcium/calmodulin dependen... | XR_948303.2 | 13.4% | (many diffs) | |
| 9 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | NM_009793.3 | 82.8% | 80% | (many diffs) |
| 10 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | XM_006525544.3 | 82.8% | 80% | (many diffs) |
| 11 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | XM_006525546.3 | 82.8% | 80% | (many diffs) |
| 12 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | XM_006525543.2 | 78.3% | 75.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct caaagtcacg gtgccctcct gctccgcctc gtcctgctct tcggtcaccg 121 ccagtgcggc cccggggacc gcgagcctcg tcccggatta ctggatcgac ggctccaaca 181 gggatgcgct gagcgatttc ttcgaggtgg agtcggagct gggacggggt gctacatcca 241 ttgtgtacag atgcaaacag aaggggaccc agaagcctta tgctctcaaa gtgttaaaga 301 aaacagtgga caaaaaaatc gtaagaactg agataggagt tcttcttcgc ctctcacatc 361 caaacattat aaaacttaaa gagatatttg aaacccctac agaaatcagt ctggtcctag 421 aactcgtcac aggaggagaa ctgtttgata ggattgtgga aaagggatat tacagtgagc 481 gagatgctgc agatgccgtt aaacaaatcc tggaggcagt tgcttatcta catgaaaatg 541 ggattgtcca tcgtgatctc aaaccagaga atcttcttta tgcaactcca gccccagatg 601 caccactcaa aatcgctgat tttggactct ctaaaattgt ggaacatcaa gtgctcatga 661 agacagtatg tggaacccca gggtactgcg cacctgaaat tcttagaggt tgtgcctatg 721 gacctgaggt ggacatgtgg tctgtaggaa taatcaccta catcttactt tgtggatttg 781 aaccattcta tgatgaaaga ggcgatcagt tcatgttcag gagaattctg aattgtgaat 841 attactttat ctccccctgg tgggatgaag tatctctaaa tgccaaggac ttggtcagaa 901 aattaattgt tttggatcca aagaaacggc tgactacatt tcaagctctc cagcatccgt 961 gggtcacagg taaagcagcc aattttgtac acatggatac cgctcaaaag aagctccaag 1021 aattcaatgc ccggcgtaag cttaaggcag cggtgaaggc tgtggtggcc TCTTCCCGCC 1081 TGGGAAGTGC CAGCAGCAGC CATGGCAGCA TCCAGGAGAG CCACAAGGCT AGCCGAGACC 1141 CTTCTCCAAT CCAAGATGGC AACGAGGACA TGAAAGCTAT TCCAGAAGGA GAGAAAATTC 1201 AAGGCGATGG GGCCCAAGCC GCAGTTAAGG GGGCACAGGC TGAGCTGATG AAGGTGCAAG 1261 CCTTAGAGAA AGTTAAAGGT GCAGATATAA ATGCTGAAGA GGCCCCCAAA ATGGTGCCCA 1321 AGGCAGTGGA GGATGGGATA AAGGTGGCTG ACCTGGAACT AGAGGAGGGC CTAGCAGAGG 1381 AGAAGCTGAA GACTGTGGAG GAGGCAGCAG CTCCCAGAGA AGGGCAAGGA AGCTCTGCTG 1441 TGGGTTTTGA AGTTCCACAG CAAGATGTGA TCCTGCAGAG TACTACCCAA CTTTCTTGTA 1501 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 1561 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 1621 AAAGGACGAC AAAGATTTTA GCGCCCGCTT TCGACGCGTT AAGTCgacaa tcaacctctg 1681 gattacaaaa tttgtgaaag att