Construct: ORF TRCN0000480970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010210.1_s317c1
- Derived from:
- ccsbBroadEn_11021
- DNA Barcode:
- ACGACCGTAACAGTGTGTCGCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCMT1 (5110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480970
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001360456.1 | 100% | 100% | |
| 2 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001360452.1 | 98.8% | 98.6% | 675_676delGT;680A>T;681_682insAATTG |
| 3 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | XM_011535868.2 | 94.2% | 92.1% | (many diffs) |
| 4 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | XM_024446451.1 | 93% | 90.8% | (many diffs) |
| 5 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001252049.1 | 79.7% | 79.7% | 1_174del |
| 6 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001252053.1 | 78.8% | 78.6% | (many diffs) |
| 7 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_005389.2 | 78.8% | 78.6% | (many diffs) |
| 8 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001252052.1 | 67.4% | 67.4% | 1_174del;363_364ins105 |
| 9 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001252050.1 | 66.6% | 66.4% | (many diffs) |
| 10 | human | 5110 | PCMT1 | protein-L-isoaspartate (D-a... | NM_001252051.1 | 66.6% | 66% | (many diffs) |
| 11 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_017313832.1 | 82.9% | 87.7% | (many diffs) |
| 12 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_017313833.1 | 82% | 86.4% | (many diffs) |
| 13 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_006512594.2 | 77.1% | 82.4% | (many diffs) |
| 14 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_011243140.1 | 77.1% | 82.4% | (many diffs) |
| 15 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_011243141.2 | 77.1% | 82.4% | (many diffs) |
| 16 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_017313834.1 | 76.2% | 81.1% | (many diffs) |
| 17 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_017313835.1 | 76.2% | 81.1% | (many diffs) |
| 18 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | NM_001347228.1 | 72.1% | 76.5% | (many diffs) |
| 19 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | NM_008786.2 | 71.3% | 75.5% | (many diffs) |
| 20 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_006512595.3 | 64.1% | 68.8% | (many diffs) |
| 21 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_017313836.1 | 64.1% | 68.8% | (many diffs) |
| 22 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_017313837.1 | 63.2% | 67.5% | (many diffs) |
| 23 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_006512589.3 | 58.9% | 56.7% | (many diffs) |
| 24 | mouse | 18537 | Pcmt1 | protein-L-isoaspartate (D-a... | XM_006512590.2 | 53% | 55.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggaaatcc ggcggcgcca gccactcgga gctaatccac aatctccgca 121 aaaatggaat catcaagaca gataaagtat ttgaagtgat gctggctaca gaccgctccc 181 actatgcaaa atgtaaccca tacatggatt ctccacaatc aataggtttc caagcaacaa 241 tcagtgctcc acacatgcat gcatatgcgc tagaacttct atttgatcag ttgcatgaag 301 gagctaaagc tcttgatgta ggatctggaa gtggaatcct tactgcatgt tttgcacgta 361 tggttGGATG TACTGGAAAA GTCATAGGAA TTGATCACAT TAAAGAGCTA GTAGATGACT 421 CAGTAAATAA TGTCAGGAAG GACGATCCAA CACTTCTGTC TTCAGGGAGA GTACAGCTTG 481 TTGTGGGGGA TGGAAGAATG GGATATGCTG AAGAAGCCCC TTATGATGCC ATTCATGTGG 541 GAGCTGCAGC CCCTGTTGTA CCCCAGGCGC TAATAGATCA GTTAAAGCCC GGAGGAAGAT 601 TGATATTGCC TGTTGGTCCT GCAGGCGGAA ACCAAATGTT GGAGCAGTAT GACAAGCTAC 661 AAGATGGCAG CATCAAAATG AAGCCTCTGA TGGGGGTGAT ATACGTGCCT TTAACAGATA 721 AAGAAAAGCA GTGGTCCAGG GATGAATTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGAC 901 CGTAACAGTG TGTCGCAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt