Construct: ORF TRCN0000480991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018989.2_s317c1
- Derived from:
- ccsbBroadEn_14633
- DNA Barcode:
- TGCACGGACTTGATTTCCTAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERBB4 (2066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480991
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | NM_005235.3 | 99.9% | 100% | 3923_3924delTG |
| 2 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_006712364.3 | 98.8% | 98.8% | 1871_1915del;3968_3969delTG |
| 3 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_005246376.3 | 98.8% | 98.5% | (many diffs) |
| 4 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | NM_001042599.1 | 98.7% | 98.7% | 3135_3136ins48;3875_3876delTG |
| 5 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003578.2 | 98% | 98% | 557_634del;4001_4002delTG |
| 6 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_005246377.3 | 97.5% | 97.3% | (many diffs) |
| 7 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003577.2 | 96.9% | 96.9% | 557_634del;1949_1993del;4046_4047delTG |
| 8 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003580.2 | 96.8% | 96.6% | (many diffs) |
| 9 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003581.2 | 96.8% | 96.8% | 557_634del;3213_3214ins48;3953_3954delTG |
| 10 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003579.2 | 95.7% | 95.7% | (many diffs) |
| 11 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003582.1 | 83.1% | 83.1% | 1_1delAins622;1250_1294del;3347_3348delTG |
| 12 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495692.3 | 89.9% | 97.3% | (many diffs) |
| 13 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495693.3 | 88.8% | 95.9% | (many diffs) |
| 14 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | NM_010154.2 | 88.7% | 96.1% | (many diffs) |
| 15 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495694.3 | 87.6% | 94.7% | (many diffs) |
| 16 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495695.3 | 57.5% | 62.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3993
- ORF length:
- 3924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagccggcg acaggacttt gggtctgggt gagccttctc gtggcggcgg 121 ggaccgtcca gcccagcgat tctcagtcag tgtgtgcagg aacggagaat aaactgagct 181 ctctctctga cctggaacag cagtaccgag ccttgcgcaa gtactatgaa aactgtgagg 241 ttgtcatggg caacctggag ataaccagca ttgagcacaa ccgggacctc tccttcctgc 301 ggtctgttcg agaagtcaca ggctacgtgt tagtggctct taatcagttt cgttacctgc 361 ctctggagaa tttacgcatt attcgtggga caaaacttta tgaggatcga tatgccttgg 421 caatattttt aaactacaga aaagatggaa actttggact tcaagaactt ggattaaaga 481 acttgacaga aatcctaaat ggtggagtct atgtagacca gaacaaattc ctttgttatg 541 cagacaccat tcattggcaa gatattgttc ggaacccatg gccttccaac ttgactcttg 601 tgtcaacaaa tggtagttca ggatgtggac gttgccataa gtcctgtact ggccgttgct 661 ggggacccac agaaaatcat tgccagactt tgacaaggac ggtgtgtgca gaacaatgtg 721 acggcagatg ctacggacct tacgtcagtg actgctgcca tcgagaatgt gctggaggct 781 gctcaggacc taaggacaca gactgctttg cctgcatgaa tttcaatgac agtggagcat 841 gtgttactca gtgtccccaa acctttgtct acaatccaac cacctttcaa ctggagcaca 901 atttcaatgc aaagtacaca tatggagcat tctgtgtcaa gaaatgtcca cataactttg 961 tggtagattc cagttcttgt gtgcgtgcct gccctagttc caagatggaa gtagaagaaa 1021 atgggattaa aatgtgtaaa ccttgcactg acatttgccc aaaagcttgt gatggcattg 1081 gcacaggatc attgatgtca gctcagactg tggattccag taacattgac aaattcataa 1141 actgtaccaa gatcaatggg aatttgatct ttctagtcac tggtattcat ggggaccctt 1201 acaatgcaat tgaagccata gacccagaga aactgaacgt ctttcggaca gtcagagaga 1261 taacaggttt cctgaacata cagtcatggc caccaaacat gactgacttc agtgtttttt 1321 ctaacctggt gaccattggt ggaagagtac tctatagtgg cctgtccttg cttatcctca 1381 agcaacaggg catcacctct ctacagttcc agtccctgaa ggaaatcagc gcaggaaaca 1441 tctatattac tgacaacagc aacctgtgtt attatcatac cattaactgg acaacactct 1501 tcagcacaat caaccagaga atagtaatcc gggacaacag aaaagctgaa aattgtactg 1561 ctgaaggaat ggtgtgcaac catctgtgtt ccagtgatgg ctgttgggga cctgggccag 1621 accaatgtct gtcgtgtcgc cgcttcagta gaggaaggat ctgcatagag tcttgtaacc 1681 tctatgatgg tgaatttcgg gagtttgaga atggctccat ctgtgtggag tgtgaccccc 1741 agtgtgagaa gatggaagat ggcctcctca catgccatgg accgggtcct gacaactgta 1801 caaagtgctc tcattttaaa gatggcccaa actgtgtgga aaaatgtcca gatggcttac 1861 agggggcaaa cagtttcatt ttcaagtatg ctgatccaga tcgggagtgc cacccatgcc 1921 atccaaactg cacccaaggg tgtaacggtc ccactagtca tgactgcatt tactacccat 1981 ggacgggcca ttccacttta ccacaacatg ctagaactcc cctgattgca gctggagtaa 2041 ttggtgggct cttcattctg gtcattgtgg gtctgacatt tgctgtttat gttagaagga 2101 agagcatcaa aaagaaaaga gccttgagaa gattcttgga aacagagttg gtggaaccat 2161 taactcccag tggcacagca cccaatcaag ctcaacttcg tattttgaaa gaaactgagc 2221 tgaagagggt aaaagtcctt ggctcaggtg cttttggaac ggtttataaa ggtatttggg 2281 tacctgaagg agaaactgtg aagattcctg tggctattaa gattcttaat gagacaactg 2341 gtcccaaggc aaatgtggag ttcatggatg aagctctgat catggcaagt atggatcatc 2401 cacacctagt ccggttgctg ggtgtgtgtc tgagcccaac catccagctg gttactcaac 2461 ttatgcccca tggctgcctg ttggagtatg tccacgagca caaggataac attggatcac 2521 aactgctgct taactggtgt gtccagatag ctaagggaat gatgtacctg gaagaaagac 2581 gactcgttca tcgggatttg gcagcccgta atgtcttagt gaaatctcca aaccatgtga 2641 aaatcacaga ttttgggcta gccagactct tggaaggaga tgaaaaagag tacaatgctg 2701 atggaggaaa gatgccaatt aaatggatgg ctctggagtg tatacattac aggaaattca 2761 cccatcagag tgacgtttgg agctatggag ttactatatg ggaactgatg acctttggag 2821 gaaaacccta tgatggaatt ccaacgcgag aaatccctga tttattagag aaaggagaac 2881 gtttgcctca gcctcccatc tgcactattg acgtttacat ggtcatggtc aaatgttgga 2941 tgattgatgc tgacagtaga cctaaattta aggaactggc tgctgagttt tcaaggatgg 3001 ctcgagaccc tcaaagatac ctagttattc agggtgatga tcgtatgaag cttcccagtc 3061 caaatgacag caagttcttt cagaatctct tggatgaaga ggatttggaa gatatgatgg 3121 atgctgagga gtacttggtc cctcaggctt tcaacatccc acctcccatc tatacttcca 3181 gagcaagaat tgactcgaat aggagtgaaa ttggacacag ccctcctcct gcctacaccc 3241 ccatgtcagg aaaccagttt gtataccgag atggaggttt tgctgctgaa caaggagtgt 3301 ctgtgcccta cagagcccca actagcacaa ttccagaagc tcctgtggca cagggtgcta 3361 ctgctgagat ttttgatgac tcctgctgta atggcaccct acgcaagcca gtggcacccc 3421 atgtccaaga ggacagtagc acccagaggt acagtgctga ccccaccgtg tttgccccag 3481 aacggagccc acgaggagag ctggatgagg aaggttacat gactcctatg cgagacaaac 3541 ccaaacaaga atacctgaat ccagtggagg agaacccttt tgtttctcgg agaaaaaatg 3601 gagaccTTCA AGCATTGGAT AATCCCGAAT ATCACAATGC ATCCAATGGT CCACCCAAGG 3661 CCGAGGATGA GTATGTGAAT GAGCCACTGT ACCTCAACAC CTTTGCCAAC ACCTTGGGAA 3721 AAGCTGAGTA CCTGAAGAAC AACATACTGT CAATGCCAGA GAAGGCCAAG AAAGCGTTTG 3781 ACAACCCTGA CTACTGGAAC CACAGCCTGC CACCTCGGAG CACCCTTCAG CACCCAGACT 3841 ACCTGCAGGA GTACAGCACA AAATATTTTT ATAAACAGAA TGGGCGGATC CGGCCTATTG 3901 TGGCAGAGAA TCCTGAATAC CTCTCTGAGT TCTCCCTGAA GCCAGGCACT GTGCTGCCGC 3961 CTCCACCTTA CAGACACCGG AATACTGTGG TGCCAACTTT CTTGTACAAA GTGGTTGATA 4021 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 4081 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCAC 4141 GGACTTGATT TCCTAAATAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 4201 tgaaagatt