Construct: ORF TRCN0000480993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018395.1_s317c1
- Derived from:
- ccsbBroadEn_08087
- DNA Barcode:
- GATCGGGACTTACTATGTTTATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3C (27350)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480993
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27350 | APOBEC3C | apolipoprotein B mRNA editi... | NM_014508.3 | 99.8% | 99.4% | 91A>G |
| 2 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_001363781.1 | 80.4% | 70.4% | (many diffs) |
| 3 | human | 27350 | APOBEC3C | apolipoprotein B mRNA editi... | XM_024452218.1 | 72% | 61.8% | (many diffs) |
| 4 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | NM_145298.6 | 45.8% | 39.1% | (many diffs) |
| 5 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452179.1 | 45.8% | 39.1% | (many diffs) |
| 6 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_152426.4 | 44.4% | 37.3% | (many diffs) |
| 7 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452178.1 | 38.6% | 33% | (many diffs) |
| 8 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XM_017028596.2 | 37.7% | 31.6% | (many diffs) |
| 9 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755170.2 | 22.6% | (many diffs) | |
| 10 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755169.2 | 20.8% | (many diffs) | |
| 11 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XR_002958675.1 | 11.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tccacagatc agaaacccga tgaaggcaat gtatccaggc acattctact 121 tccaatttaa aaacctatgg gaagccaacg atcgggacga aacttggctg tgcttcaccg 181 tggaaggtat aaagcgccgc tcagttgtct cctggaagac gggcgTCTTC CGAAACCAGG 241 TGGATTCTGA GACCCATTGT CATGCAGAAA GGTGCTTCCT CTCTTGGTTC TGCGACGACA 301 TACTGTCTCC TAACACAAAG TACCAGGTCA CCTGGTACAC ATCTTGGAGC CCTTGCCCAG 361 ACTGTGCAGG GGAGGTGGCC GAGTTCCTGG CCAGGCACAG CAACGTGAAT CTCACCATCT 421 TCACCGCCCG CCTCTACTAC TTCCAGTATC CATGTTACCA GGAGGGGCTC CGCAGCCTGA 481 GTCAGGAAGG GGTCGCTGTG GAGATCATGG ACTATGAAGA TTTTAAATAT TGTTGGGAAA 541 ACTTTGTGTA CAATGATAAT GAGCCATTCA AGCCTTGGAA GGGATTAAAA ACCAACTTTC 601 GACTTCTGAA AAGAAGGCTA CGGGAGAGTC TCCAGTGCCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AGATCGGGAC TTACTATGTT TATTCACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt