Construct: ORF TRCN0000481022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002979.1_s317c1
- Derived from:
- ccsbBroadEn_11124
- DNA Barcode:
- AAGATGCAAGGGTTATGCCCTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHA (6389)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6389 | SDHA | succinate dehydrogenase com... | NM_001294332.1 | 83.4% | 82.1% | (many diffs) |
| 2 | human | 6389 | SDHA | succinate dehydrogenase com... | NM_004168.4 | 77.7% | 78% | (many diffs) |
| 3 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_011514072.2 | 73.6% | 72.7% | (many diffs) |
| 4 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_024446143.1 | 68.2% | 65% | (many diffs) |
| 5 | human | 6389 | SDHA | succinate dehydrogenase com... | NM_001330758.1 | 65.6% | 65.8% | (many diffs) |
| 6 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_017009685.2 | 64.3% | 62.5% | (many diffs) |
| 7 | human | 727956 | SDHAP2 | succinate dehydrogenase com... | NR_003265.3 | 62.9% | (many diffs) | |
| 8 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_011514073.2 | 61.9% | 60.8% | (many diffs) |
| 9 | human | 255812 | SDHAP1 | succinate dehydrogenase com... | NR_003264.2 | 57.4% | (many diffs) | |
| 10 | human | 6389 | SDHA | succinate dehydrogenase com... | XR_002956167.1 | 28.4% | (many diffs) | |
| 11 | mouse | 66945 | Sdha | succinate dehydrogenase com... | NM_023281.1 | 67% | 73.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggggtccgg ggcctgtcgc ggctgctgag cgctcggcgc ctggcgctgg 121 ccaaggcgtg gccaacagtg ttgcaaacag gaacccgagg ttttcacttc actgttgatg 181 ggaacaagag ggcatctgct aaagtttcag attccatttc tgctcagtat ccagtagtgg 241 atcatgaatt tgatgcagtg gtggtaggcg ctggaggggc aggcttgcga gctgcatttg 301 gcctttctga ggcagggttt aatacagcat gtgttaccaa gctgtttcct accaggtcac 361 acactgttgc agcgcaggga ggaatcaatg ctgctctggg gaacatggag gaggacaact 421 ggaggtggca tttctacgac accagcactg gcgacggcac ggccatgatc accagggcag 481 gccttccttg ccaggaccta gagtttgttc agttccaccc cacaggcata tatggtgctg 541 gttgtctcat tacggaagga tgtcgtggag agggaggcat tctcattaac agtcaaggcg 601 aaaggtttat ggagcgatac gcccctgtcg cgaaggacct ggcgtctaga gatgtggtgt 661 ctcggtcgat gactctggag atccgagaag gaagaggctg tggccctgag aaagatcacg 721 tctacctgca gctgcaccac ctacctccag agcagctggc cacgcgcctg cctggcattt 781 cagagacagc catgatcttt gctggcgtgg acgtcacgaa ggagccgatc cctgtcctcc 841 ccaccgtgca ttataacatg ggcggcattc ccaccaacta caaggggcag gtcctgaggc 901 acgtgaatgg ccaggatcag attgtgcccg gcctgtacgc ctgtggggag gccgcctgtg 961 cctcggtaca tggtgccaac cgcctcgggg caaactcgct cttggacctg gttgtctttg 1021 gtcgggcatg tgccctgagc atcgaagagt catgcaggcc tggagataaa gtccctccaa 1081 ttaaaccaaa cgctggggaa gaatctgtca tgaatcttga caaattgaga tttgctgatg 1141 gaagcataag aacatcggaa ctgcgactca gcatgcagaa gtcaatgcaa aatcatgctg 1201 ccgtgttccg tgtgggaagc gtgttgcaag aaggttgtgg gaaaaTCAGC AAGCTCTATG 1261 GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACA GACCTGGTGG 1321 AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC GGAGCAGAGG 1381 CGCGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG ATTGATGAGT 1441 ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG CACTGGAGGA 1501 AGCACACCCT GTCCTTTGTG GACGTTGGCA CTGGGAAGGT CACTCTGGAA TATAGACCCG 1561 TAATCGACAA AACTTTGAAC GAGGCTGACT GTGCCACCGT CCCGCCAGCC ATTCGCTCCT 1621 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAAA GATGCAAGGG TTATGCCCTG CCACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt