Construct: ORF TRCN0000481038
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014178.1_s317c1
- Derived from:
- ccsbBroadEn_10630
- DNA Barcode:
- GTGACAGGGAACAATCAAACCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT14P1 (401135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481038
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000935.2 | 50.4% | 43.2% | (many diffs) |
| 2 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001256006.1 | 33% | 28.4% | (many diffs) |
| 3 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000932.2 | 31.9% | 27.4% | (many diffs) |
| 4 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000933.2 | 31.9% | 27.4% | (many diffs) |
| 5 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000934.2 | 31.9% | 27.4% | (many diffs) |
| 6 | human | 255928 | SYT14 | synaptotagmin 14 | NM_153262.4 | 30.8% | 26.4% | (many diffs) |
| 7 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001146262.3 | 29.8% | 25.6% | (many diffs) |
| 8 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001146264.3 | 28.5% | 24.5% | (many diffs) |
| 9 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001146261.2 | 27.6% | 23.7% | (many diffs) |
| 10 | human | 401135 | SYT14P1 | synaptotagmin 14 pseudogene 1 | NR_027094.1 | 21.4% | 1_189del;276C>T;754_2628del | |
| 11 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000931.1 | 20.2% | 17.4% | (many diffs) |
| 12 | human | 255928 | SYT14 | synaptotagmin 14 | XM_006711262.3 | 19.8% | 17% | (many diffs) |
| 13 | human | 255928 | SYT14 | synaptotagmin 14 | NR_027459.3 | 4.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 633
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggagggc ttccagatct tggttcagaa tacagtacaa ggaacaattc 121 acaagataaa atgtgtctgt gactcccaaa tgagtgtgtc agaaatgtct tgtagtgaaa 181 gtacatccgc atgtcagtct ctcgaagacg ggtcagttcc agaaatTCTC ATTAGCCTGC 241 TTTATAATGC CACAAATGGG AGACTATCAG CAGAAGTGAT AAAAGGCAGC CACTTAAAAA 301 ATTGGGCAGC AAACAGACCA CCCAATACAT ATGTTAAGTT AACTCTACGG AAATCCACGG 361 ATCAAGAGAT GTCCAAATGC AAGATATCCA TCCGCAGAGG GCGGCCAAAT CCAGTATACA 421 AGGAAACTTT TGTTTTCAAA GTGACCCTAT TTCAGCTTTC TCATGTGACA CTCATGCTGT 481 CTGTGTATAA CAAAAGCAGC ATGAGAAGAA AGATGATAGG CTGGATTTAT TTAGGTCTCA 541 ACAGCTCTGG AGAAGAACTC AATCACTGGA CTGAAATGAA AGAGTCAAAA GGACGGCAAG 601 TACGTAGATG GCAGGCGTTG CTAGAGTCAC GATTGCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 781 GACAGGGAAC AATCAAACCC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt