Construct: ORF TRCN0000481066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019045.3_s317c1
- Derived from:
- ccsbBroadEn_14630
- DNA Barcode:
- ACATACGAGACCCATCCACGAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB6 (2051)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2051 | EPHB6 | EPH receptor B6 | NM_004445.6 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515879.1 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515880.1 | 99.8% | 99.8% | (many diffs) |
| 4 | human | 2051 | EPHB6 | EPH receptor B6 | XM_024446674.1 | 99.8% | 99.8% | (many diffs) |
| 5 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515881.3 | 99.7% | 99.7% | (many diffs) |
| 6 | human | 2051 | EPHB6 | EPH receptor B6 | XM_011515882.2 | 96.7% | 96.7% | (many diffs) |
| 7 | human | 2051 | EPHB6 | EPH receptor B6 | XM_024446675.1 | 96.6% | 96.6% | (many diffs) |
| 8 | human | 2051 | EPHB6 | EPH receptor B6 | NR_104001.2 | 77.3% | (many diffs) | |
| 9 | human | 2051 | EPHB6 | EPH receptor B6 | NM_001280794.2 | 71.3% | 71.3% | (many diffs) |
| 10 | human | 2051 | EPHB6 | EPH receptor B6 | NM_001280795.2 | 71.3% | 71.3% | (many diffs) |
| 11 | mouse | 13848 | Ephb6 | Eph receptor B6 | NM_001146351.1 | 86.4% | 91.9% | (many diffs) |
| 12 | mouse | 13848 | Ephb6 | Eph receptor B6 | NM_007680.4 | 86.4% | 91.9% | (many diffs) |
| 13 | mouse | 13848 | Ephb6 | Eph receptor B6 | XR_377419.3 | 40.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3132
- ORF length:
- 3063
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctactgaa ggggctgccc agttagggaa cagagtggcg ggcatggtgt 121 gtagcctatg ggtgctgctc ctggtgtctt cagttctggc tctggaagag gtattgctgg 181 acaccaccgg agagacatct gagattggct ggctcaccta cccaccaggg gggtgggacg 241 aggtgagtgt tctggacgac cagcgacgcc tgactcggac ctttgaggca tgtcatgtgg 301 caggggcccc tccaggcacc gggcaggaca attggttgca gacacacttt gtggagcggc 361 gcggggccca gagggcgcac attcgactcc acttctctgt gcgggcatgc tccagcctgg 421 gtgtgagcgg cggcacctgc cgggagacct tcacccttta ctaccgtcag gctgaggagc 481 ccgacagccc tgacagcgtt tcctcctggc acctcaaacg ctggaccaag gtggacacaa 541 ttgcagcaga cgagagcttt ccctcctcct cctcctcctc ctcctcttct tcctctgcag 601 cgtgggctgt gggaccccac ggggctgggc agcgggctgg actgcaactg aacgtcaaag 661 agcggagctt tgggcctctc acccaacgcg gcttctacgt ggccttccag gacacggggg 721 cctgcctggc cctggtcgct gtcaggctct tctcctacac ctgccctgcc gtgctccgat 781 cctttgcttc ctttccagag acgcaggcca gtggggctgg gggggcctcc ctggtggcag 841 ctgtgggcac ctgtgtggct catgcagagc cagaggagga tggagtaggg ggccaggcag 901 gaggcagccc ccccaggctg cactgcaacg gggagggcaa gtggatggta gctgtcgggg 961 gctgccgctg ccagcctgga taccaaccag cacgaggaga caaggcctgc caagcctgcc 1021 cacgggggct ctataagtct tctgctggga atgctccctg ctcaccatgc cctgcccgca 1081 gtcacgctcc caacccagca gcccccgttt gcccctgcct ggagggcttc taccgggcca 1141 gttccgaccc accagaggcc ccctgcactg gtcctccatc ggctccccag gagctttggt 1201 ttgaggtgca aggctcagca ctcatgctac actggcgcct gcctcgggag ctggggggtc 1261 gaggggacct gctcttcaat gtcgtgtgca aggagtgtga aggccgccag gaacctgcca 1321 gcggtggtgg gggcacttgt caccgctgca gggatgaggt ccacttcgac cctcgccaga 1381 gaggcctgac tgagagccga gtgttagtgg ggggactccg ggcacacgta ccctacatct 1441 tagaggtgca ggctgttaat ggggtgtctg agctcagccc tgaccctcct caggctgcag 1501 ccatcaatgt cagcaccagc catgaagtgc cctctgctgt ccctgtggtg caccaggtga 1561 gccgggcatc caacagcatc acggtgtcct ggccgcagcc cgaccagacc aatgggaaca 1621 tcctggacta tcagctccgc tactatgacc aggcagaaga cgaatcccac tccttcaccc 1681 tgaccagcga gaccaacact gccaccgtga cacagctgag ccctggccac atctatggtt 1741 tccaggtgcg ggcccggact gctgccggcc acggccccta cgggggcaaa gtctatttcc 1801 agacacttcc tcaaggggag ctgtcttccc agcttccgga aagactctcc ttggtgatcg 1861 gctccatcct gggggctttg gccttcctcc tgctggcagc catcaccgtg ctggcggtcg 1921 tcttccagcg gaagcggcgt gggactggct acacggagca gctgcagcaa tacagcagcc 1981 caggactcgg ggtgaagtat tacatcgacc cctccaccta cgaggacccc tgtcaggcca 2041 tccgagaact tgcccgggaa gtcgatcctg cttatatcaa gattgaggag gtcattggga 2101 caggctcttt tggagaagtg cgccagggcc gcctgcagcc acggggacgg agggagcaga 2161 ctgtggccat ccaggccctg tgggccgggg gcgccgaaag cctgcagatg accttcctgg 2221 gccgggccgc agtgctgggt cagttccagc accccaacat cctgcggctg gagggcgtgg 2281 tcaccaagag ccgacccctc atggtgctga cggagttcat ggagcttggc cccctggaca 2341 gcttcctcag gcagcgggag ggccagttca gcagcctgca gctggtggcc atgcagcggg 2401 gagtggctgc tgccatgcag tacctgtcca gctttgcctt cgtccatcgc tcgctgtctg 2461 cccacagcgt gctggtgaat agccacttgg tgtgcaaggt ggcccgtctt ggccacagtc 2521 ctcagggccc aagttgtttg cttcgctggg cagccccaga ggtcattgca catggaaagc 2581 atacaacatc cagtgatgtc tggagctttg ggatactcat gtgggaagtg atgagttatg 2641 gagaacggcc ttactgggac atgagtgagc aggaggtact aaatgcaata gagcaggagt 2701 tccggctgcc cccgcctcca ggctgtcctc ctggattaca tctacttatg ttggacactt 2761 ggcagaagga ccgtgcccgg cggCCTCATT TTGACCAGCT GGTGGCTGCA TTTGACAAGA 2821 TGATCCGCAA GCCAGATACC CTGCAGGCTG GCGGGGACCC AGGGGAAAGG CCTTCCCAGG 2881 CCCTTCTGAC CCCTGTGGCC CTGGACTTTC CTTGTCTGGA CTCACCCCAG GCCTGGCTTT 2941 CAGCCATTGG ACTGGAGTGC TACCAGGACA ACTTCTCCAA GTTTGGCCTC TGTACCTTCA 3001 GTGATGTGGC TCAGCTCAGC CTAGAAGACC TGCCTGCCCT GGGCATCACC CTGGCTGGCC 3061 ACCAGAAGAA GCTGCTGCAC CACATCCAGC TCCTTCAGCA ACACCTGAGG CAGCAGGGCT 3121 CAGTGGAGGT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3181 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3241 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA TACGAGACCC ATCCACGAAG 3301 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t