Construct: ORF TRCN0000481112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008042.1_s317c1
- Derived from:
- ccsbBroadEn_14097
- DNA Barcode:
- TAATGATCTATAGGGTACTATCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTHFD1L (25902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350490.1 | 73.4% | 73.2% | 1_1delAins289;214A>G;372C>G |
| 2 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350487.1 | 42% | 42% | 1_1506del;2008A>G;2166C>G |
| 3 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350492.1 | 42% | 42% | 1_1509del;2011A>G;2169C>G |
| 4 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350493.1 | 42% | 42% | 1_1509del;2011A>G;2169C>G |
| 5 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001350486.1 | 40% | 40% | 1_1638del;2140A>G;2298C>G |
| 6 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535734.2 | 40% | 38.1% | (many diffs) |
| 7 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_017010705.1 | 40% | 38.1% | (many diffs) |
| 8 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001242768.1 | 40% | 39.9% | 1_1641del;2143A>G;2301C>G |
| 9 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535732.2 | 40% | 38.1% | (many diffs) |
| 10 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535733.2 | 40% | 38.1% | (many diffs) |
| 11 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_024446395.1 | 38.7% | 38.7% | 1_1731del;2233A>G;2391C>G |
| 12 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535731.2 | 38.1% | 36.3% | (many diffs) |
| 13 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535730.2 | 38.1% | 36.3% | (many diffs) |
| 14 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_015440.4 | 37.3% | 37.3% | 1_1836del;2338A>G;2496C>G |
| 15 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NM_001242767.1 | 37.3% | 37.2% | 1_1839del;2341A>G;2499C>G |
| 16 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_017010702.2 | 37.3% | 37.2% | 1_1839del;2341A>G;2499C>G |
| 17 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_017010703.2 | 37.3% | 37.2% | 1_1839del;2341A>G;2499C>G |
| 18 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_005266907.5 | 35.6% | 33.9% | (many diffs) |
| 19 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XM_011535729.3 | 34.4% | 34.4% | (many diffs) |
| 20 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XR_001743322.2 | 31.1% | (many diffs) | |
| 21 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NR_146719.1 | 30.6% | (many diffs) | |
| 22 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | XR_002956274.1 | 30.4% | (many diffs) | |
| 23 | human | 101929583 | LOC101929583 | methylenetetrahydrofolate d... | NR_136299.1 | 28.8% | (many diffs) | |
| 24 | human | 25902 | MTHFD1L | methylenetetrahydrofolate d... | NR_146720.1 | 24.9% | (many diffs) | |
| 25 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | NM_001170785.1 | 32.7% | 35.9% | (many diffs) |
| 26 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | NM_001170786.1 | 32.7% | 35.9% | (many diffs) |
| 27 | mouse | 270685 | Mthfd1l | methylenetetrahydrofolate d... | NM_172308.4 | 32.7% | 35.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1164
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtgctggcc ctgacggaca gcctcgcaga catgaaggca cggctgggaa 121 ggatggtggt ggccagtgac aaaagcgggc agcctgtgac agcagatgat ttgggggtga 181 caggtgcttt gacagttttg atgaaagatg caataaaacc aaacctgatg cagaccctgg 241 aagggacacc tgtgttcgtg catgcgggcc cttttgctaa cattgctcac ggcaactctt 301 cagtgttggc tgataaaatt gccctgaaac tggttggtga agaaggattt gtagtgaccg 361 aagctggctt tggtgctgac atcggaatgg agaaattctt caacatcaag tgccgagctt 421 ccggcttggt gcccaacgtg gttgtgttag tggcaacggt gcgagctctg aagatgcatg 481 gaggcgggcc aagtgtaacg gctggtgttc ctcttaagaa agaatataca gaggagaaca 541 tccagctggt ggcagacggc tgctgtgacc tccagaagca aattcagatc actcagctct 601 ttggggttcc cgttgtggtg gctctgaatg tcttcaagac cgacacccgc gctgagattg 661 acttggtgtg tgagcttgca aagcggGCTG GTGCCTTTGA TGCAGTCCCC TGCTATCACT 721 GGTCGGTTGG TGGAAAAGGA TCGGTGGACT TGGCTCGGGC TGTGAGAGAG GCTGCGAGTA 781 AAAGAAGCCG ATTCCAGTTC CTGTATGATG TTCAGGTTCC AATTGTGGAC AAGATAAGGA 841 CCATTGCTCA GGCTGTCTAT GGAGCCAAAG ATATTGAACT CTCTCCTGAG GCACAAGCCA 901 AAATAGATCG TTACACTCAA CAGGGTTTTG GAAATTTGCC CATCTGCATG GCAAAGACCC 961 ACCTTTCTCT ATCTCACCAA CCTGACAAAA AAGGTGTGCC AAGGGACTTC ATCTTACCTA 1021 TCAGTGACGT CCGGGCCAGC ATAGGCGCTG GGTTCATTTA CCCTTTGGTC GGAACGATGA 1081 GCACCATGCC AGGACTGCCC ACCCGGCCCT GCTTTTATGA CATAGATCTT GATACCGAAA 1141 CAGAACAAGT TAAAGGCTTG TTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATGATCTAT 1321 AGGGTACTAT CCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att