Transcript: Human XM_006711275.4

PREDICTED: Homo sapiens UTP25 small subunit processor component (UTP25), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UTP25 (27042)
Length:
8122
CDS:
93..1799

Additional Resources:

NCBI RefSeq record:
XM_006711275.4
NBCI Gene record:
UTP25 (27042)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006711275.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000271465 TTACGGTTTGTTACATCATTA pLKO_005 3066 3UTR 100% 13.200 18.480 N UTP25 n/a
2 TRCN0000271513 TTCTATGAACTGCCGACATAT pLKO_005 1792 CDS 100% 13.200 18.480 N UTP25 n/a
3 TRCN0000116060 GCTACTCAACACCCTAACTAA pLKO.1 125 CDS 100% 5.625 7.875 N UTP25 n/a
4 TRCN0000116058 CCTCAATAATTGGTCCAAGTA pLKO.1 1652 CDS 100% 4.950 6.930 N UTP25 n/a
5 TRCN0000116061 CCACGAGCTTAAATGGCCTAT pLKO.1 731 CDS 100% 4.050 5.670 N UTP25 n/a
6 TRCN0000271510 CCGTATTTGTGGGCAATATTG pLKO_005 1336 CDS 100% 13.200 10.560 N UTP25 n/a
7 TRCN0000271512 TATTGCCTGCATGTGATAAAT pLKO_005 1020 CDS 100% 15.000 10.500 N UTP25 n/a
8 TRCN0000116059 CCCAAAGGATATTGACTTAAA pLKO.1 806 CDS 100% 13.200 9.240 N UTP25 n/a
9 TRCN0000116057 CGCTGGACTTTAATGAGATAA pLKO.1 6818 3UTR 100% 13.200 9.240 N UTP25 n/a
10 TRCN0000092942 GAAGATGAGGAGGAGGAAGAA pLKO.1 354 CDS 100% 4.950 2.475 Y Gm13232 n/a
11 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 4038 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006711275.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08048 pDONR223 100% 74.7% 73.6% None (many diffs) n/a
2 ccsbBroad304_08048 pLX_304 0% 74.7% 73.6% V5 (many diffs) n/a
3 TRCN0000481121 TAGCACCTCAGTGACGGCCTCTGG pLX_317 21.7% 74.7% 73.6% V5 (many diffs) n/a
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