Transcript: Mouse XR_373895.2

PREDICTED: Mus musculus digestive organ expansion factor homolog (zebrafish) (Diexf), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Diexf (215193)
Length:
2655
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_373895.2
NBCI Gene record:
Diexf (215193)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_373895.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249977 CTTTGTGCGTCTCCGAAATTA pLKO_005 1980 3UTR 100% 15.000 21.000 N Diexf n/a
2 TRCN0000249976 CACCAGGCCTAAGGTACTTAT pLKO_005 1183 3UTR 100% 13.200 10.560 N Diexf n/a
3 TRCN0000257968 TACTGCCTGCATGCCATAAAC pLKO_005 1061 3UTR 100% 13.200 9.240 N Diexf n/a
4 TRCN0000257943 CCGTTGAGCTGCTCATCATTG pLKO_005 1554 3UTR 100% 10.800 7.560 N Diexf n/a
5 TRCN0000156756 GAGGAAGAGGAAGAGGAAGAT pLKO.1 392 3UTR 100% 4.950 2.475 Y NPM2 n/a
6 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 390 3UTR 100% 4.950 2.475 Y Adam32 n/a
7 TRCN0000152515 GAAGAGGAAGAGGAAGATGAT pLKO.1 395 3UTR 100% 4.950 2.475 Y NPM2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_373895.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08048 pDONR223 100% 71.9% None (many diffs) n/a
2 ccsbBroad304_08048 pLX_304 0% 71.9% V5 (many diffs) n/a
3 TRCN0000481121 TAGCACCTCAGTGACGGCCTCTGG pLX_317 21.7% 71.9% V5 (many diffs) n/a
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