Construct: ORF TRCN0000481239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017632.1_s317c1
- Derived from:
- ccsbBroadEn_10457
- DNA Barcode:
- TCCCATTTCGAGTAAGGGAGCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITSN2 (50618)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50618 | ITSN2 | intersectin 2 | NM_019595.4 | 99.9% | 99.8% | 1_3delATG;4576A>C |
| 2 | human | 50618 | ITSN2 | intersectin 2 | XM_024452934.1 | 99.9% | 99.8% | 1_3delATG;4576A>C |
| 3 | human | 50618 | ITSN2 | intersectin 2 | XM_024452935.1 | 99.2% | 99.1% | 0_1ins39;4534A>C |
| 4 | human | 50618 | ITSN2 | intersectin 2 | NM_001348182.2 | 99.1% | 99.1% | 1_3delATG;2134_2135ins39;4537A>C |
| 5 | human | 50618 | ITSN2 | intersectin 2 | NM_006277.3 | 98.3% | 98.2% | 1_3delATG;1861_1941del;4657A>C |
| 6 | human | 50618 | ITSN2 | intersectin 2 | XM_024452930.1 | 98.3% | 98.2% | 1_3delATG;1861_1941del;4657A>C |
| 7 | human | 50618 | ITSN2 | intersectin 2 | XM_024452931.1 | 98.3% | 98.2% | 1_3delATG;1861_1941del;4657A>C |
| 8 | human | 50618 | ITSN2 | intersectin 2 | NM_001348181.2 | 97.6% | 97.5% | 0_1ins39;1819_1899del;4615A>C |
| 9 | human | 50618 | ITSN2 | intersectin 2 | XM_024452932.1 | 97.6% | 97.5% | 0_1ins39;1819_1899del;4615A>C |
| 10 | human | 50618 | ITSN2 | intersectin 2 | XM_024452933.1 | 97.5% | 97.5% | (many diffs) |
| 11 | human | 50618 | ITSN2 | intersectin 2 | XR_002959302.1 | 84.7% | (many diffs) | |
| 12 | human | 50618 | ITSN2 | intersectin 2 | NM_147152.3 | 71.6% | 71.3% | (many diffs) |
| 13 | human | 50618 | ITSN2 | intersectin 2 | NM_001348183.2 | 69.7% | 69.7% | 1_3delATG;3495_3496ins1515 |
| 14 | human | 50618 | ITSN2 | intersectin 2 | NM_001348184.2 | 68.9% | 68.9% | 0_1ins39;3453_3454ins1515 |
| 15 | human | 50618 | ITSN2 | intersectin 2 | NM_001348186.2 | 68.9% | 68.9% | 1_3delATG;2134_2135ins39;3456_3457ins1515 |
| 16 | human | 50618 | ITSN2 | intersectin 2 | XM_024452936.1 | 67.8% | 67.8% | 0_1ins39;1819_1899del;3534_3535ins1515 |
| 17 | human | 50618 | ITSN2 | intersectin 2 | NM_001348185.2 | 67.8% | 67.8% | (many diffs) |
| 18 | human | 50618 | ITSN2 | intersectin 2 | XM_024452937.1 | 55.4% | 54.5% | (many diffs) |
| 19 | mouse | 20403 | Itsn2 | intersectin 2 | NM_011365.4 | 87.1% | 91.7% | (many diffs) |
| 20 | mouse | 20403 | Itsn2 | intersectin 2 | NM_001198968.2 | 85.8% | 90.3% | (many diffs) |
| 21 | mouse | 20403 | Itsn2 | intersectin 2 | XM_006515023.3 | 85.8% | 90.3% | (many diffs) |
| 22 | mouse | 20403 | Itsn2 | intersectin 2 | XM_006515024.3 | 85.8% | 90.3% | (many diffs) |
| 23 | mouse | 20403 | Itsn2 | intersectin 2 | XM_006515025.3 | 85.1% | 89.6% | (many diffs) |
| 24 | mouse | 20403 | Itsn2 | intersectin 2 | XM_006515027.3 | 60.8% | 63.2% | (many diffs) |
| 25 | mouse | 20403 | Itsn2 | intersectin 2 | XM_006515026.3 | 59.8% | 62.2% | (many diffs) |
| 26 | mouse | 20403 | Itsn2 | intersectin 2 | NM_001198969.2 | 34.1% | 35% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5076
- ORF length:
- 5007
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcagttt cccacagcta tgaatggagg gccaaacatg tgggctatta 121 cctctgaaga acgtactaag catgacaggc agtttgataa cctcaaacct tcaggaggtt 181 acataacagg tgatcaagca cgtaattttt tcctacaatc aggtctgccg gcccctgttt 241 tagctgaaat atgggcttta tcagacctaa acaaggatgg gaagatggat cagcaagagt 301 tctccatagc tatgaaactc atcaaactga agcttcaagg ccaacagttg cctgtggttc 361 tccctcctat tatgaagcaa ccccctatgt tttctccatt aatttctgct cgttttggaa 421 tgggaagcat gcccaatctg tccattcctc agccattgcc tccagctgca cctataacat 481 cattgtcttc tgcgacttca gggaccaacc ttcctccctt aatgatgccc actcccctag 541 tgccttctgt tagcacatca tcattaccaa atggaaccgc cagtctcatt cagcctttac 601 ccattcctta ttcttcttca acattgcctc atgggtcatc ttatagtctg atgatgggag 661 gatttggagg tgctagtata cagaaagcgc agtctctgat tgatttagga tctagtagct 721 caacttcctc gactgcttca ctctcaggga actcacccaa gactgggacc tcagagtggg 781 cagttcctca gcctacaaga ttaaaatatc ggcaaaaatt taatactctt gacaaaagta 841 tgagtggata tctctcaggt tttcaagcta gaaatgccct tcttcagtca aatctttctc 901 aaactcagct ggctactatt tggactctgg ctgacgttga tggtgatgga cagctaaaag 961 cagaagagtt tattcttgca atgcacctta ctgacatggc caaagctgga cagccattac 1021 cactgacttt acctcctgag cttgttcctc catctttcag aggaggaaag caaattgatt 1081 ccattaatgg aactctgcct tcatatcaga aaatgcaaga agaggagcct cagaagaaat 1141 taccagttac ttttgaggac aaacggaaag ccaactatga gcgagggaac atggagctgg 1201 aaaagcgacg ccaagccttg atggagcagc aacaaaggga ggcagaacgt aaagcccaga 1261 aagaaaagga agagtgggaa cgaaaacaga gagaattaca agaacaagaa tggaagaaac 1321 aacttgaatt agaaaaacgc ttagagaagc aacgggaatt ggagagacaa cgagaggaag 1381 aaaggagaaa agacatagaa agacgagagg cagcaaaaca ggaacttgaa cgacaacgtc 1441 gcttagaatg ggagagaatt cggcgacagg agcttctcaa tcaaaagaat agagaacaag 1501 aagaaattgt caggttaaac tctaaaaaga agaatcttca tcttgagttg gaagcactga 1561 atggcaaaca tcagcagatc tcaggcagac ttcaggatgt ccgactcaaa aagcaaactc 1621 aaaagactga gctggaagtt ctggataagc agtgtgactt ggaaattatg gaaatcaagc 1681 aacttcaaca ggaacttcag gaatatcaga ataagcttat ctatctggta cctgagaagc 1741 aattattaaa tgaaagaatt aaaaacatgc agttcagtaa cacacctgat tcaggggtca 1801 gtttacttca taaaaaatca ttagaaaagg aagaattatg ccaaagactt aaagaacagt 1861 tagatgctct tgaaaaagaa actgcatcta agctgtcaga aatggattct tttaacaatc 1921 aactaaagga actgagagaa acctacaaca cacagcagtt agcccttgaa cagctttata 1981 agatcaaacg tgacaagttg aaggaaattg aaaggaaaag attagaacta atgcagaaaa 2041 agaaactaga agatgaggct gcaaggaaag caaagcaagg aaaagaaaac ttatggaaag 2101 aaaatcttag aaaggaggaa gaagaaaaac aaaagcgact ccaggaagaa aaaacacaag 2161 aaaaaattca agaagaggaa cggaaagctg aggagaaaca acgtaaggat aaggatactt 2221 tgaaagctga ggagaaaaaa cgtgagacag ctagtgtttt ggtgaattat agagcattat 2281 acccctttga agcaaggaac catgatgaga tgagttttaa ttctggagat ataattcagg 2341 ttgatgaaaa aaccgtagga gaacctggtt ggctttatgg tagttttcaa ggaaattttg 2401 gctggtttcc atgcaattat gtagaaaaaa tgccatcaag tgaaaatgaa aaagctgtat 2461 ctccaaagaa ggccttactt cctcctacag tttctttatc tgctacctca acttcctctg 2521 aaccactttc ttcaaatcaa ccagcatcag tgactgatta tcaaaatgta tctttttcaa 2581 acctaactgt aaatacatca tggcagaaaa aatcagcctt cactcgaact gtgtcccctg 2641 gatctgtatc acctattcat ggacagggac aagtggtaga aaacttaaaa gcacaggccc 2701 tttgttcctg gactgcaaag aaagataacc acttgaactt ctcaaaacat gacattatta 2761 ctgtcttgga gcagcaagaa aattggtggt ttggggaggt gcatggagga agaggatggt 2821 ttcccaaatc ttatgtcaag atcattcctg ggagtgaagt aaaacgggaa gaaccagaag 2881 ctttgtatgc agctgtaaat aagaaaccta cctcggcagc ctattcagtt ggagaagaat 2941 atattgcact ttatccatat tcaagtgtgg aacctggaga tttgactttc acagaaggtg 3001 aagaaatatt ggtgacccag aaagatggag agtggtggac aggaagtatt ggagatagaa 3061 gtggaatttt tccatcaaac tatgtcaaac caaaggatca agagagtttt gggagtgcta 3121 gcaagtctgg agcatcaaat aaaaaacctg agattgctca ggtaacttca gcatatgttg 3181 cttctggttc tgaacaactt agccttgcac caggacagtt aatattaatt ctaaagaaaa 3241 atacaagtgg gtggtggcaa ggagagttac aggccagagg aaaaaagcga cagaaaggat 3301 ggtttcctgc cagtcatgtt aaacttttgg gtccaagtag tgaaagagcc acacctgcct 3361 ttcatcctgt atgtcaggtg attgctatgt atgactatgc agcaaataat gaagatgagc 3421 tcagtttctc caagggacaa ctcattaatg ttatgaacaa agatgatcct gattggtggc 3481 aaggagagat caacggggtg actggtctct ttccttcaaa ctacgttaag atgacgacag 3541 actcagatcc aagtcaacag tggtgtgctg atctgcaaac cctggacaca atgcagccaa 3601 ttgagaggaa aagacagggc tatattcatg agctgattca gaccgaagag cggtacatgg 3661 ctgaccttca gctcgtcgtc gaggtttttc agaaacgcat ggcagagtca ggctttctca 3721 ctgaagggga gatggccctg atttttgtta actggaagga gctcatcatg tccaacacaa 3781 agctgctgaa ggctttgcgg gtgcggaaga agaccggggg cgagaagatg ccggtgcaga 3841 tgattgggga catcctggcc gctgagctgt cccacatgca ggcttacatc aggttctgca 3901 gctgccagct taatggagca gctctgttac agcagaagac agatgaagac acagatttca 3961 aagaattttt aaagaagctg gcatctgacc cgcggtgtaa aggaatgccc ctctccagct 4021 tcctgctgaa acccatgcag aggatcaccc gctacccact gctcatcaga agtattctgg 4081 agaacacccc ggagagccat gcagaccatt cctccctaaa gctggccctc gagcgggcag 4141 aggagctgtg ctctcaagtg aatgagggag ttcgggagaa ggaaaactcg gaccgactgg 4201 agtggatcca ggcgcacgtg cagtgtgaag gcctcgcgga gcaacttatt ttcaactctc 4261 tcaccaactg cctggggccc cggaagctct tacacagtgg gaaattatac aagaccaaga 4321 gcaacaagga actgcacgga ttcctcttca atgacttcct gcttcttacc tacatggtca 4381 agcagtttgc tgtttcctct ggctctgaga aacttttcag ctcgaagtcc aatgctcaat 4441 tcaaaatgta taaaacgccc attttcctga atgaagtctt ggtgaaactg cccacagacc 4501 cttccagcga tgagcctgtc ttccacattt cccacattga tcgggtctac accctccgaa 4561 cagacaacat taatgagagg accgcctggg tgcagaagat caaggcggcg tctgagcagt 4621 acatcgacac cgagaagaag cagcgtgaga aagcttacca agcccgctcc caaaagactt 4681 caggcattgg gcgcctgatg gtgcatgtca ttGAAGCTAC AGAATTAAAA GCCTGCAAAC 4741 CAAATGGAAA GAGCAACCCA TACTGTGAAA TCAGCATGGG CTCCCAGAGC TACACCACCA 4801 GGACCATCCA GGACACACTC AATCCCAAGT GGAATTTTAA CTGCCAGTTC TTTATTAAGG 4861 ATCTCTACCA AGACGTGCTG TGTCTCACCC TGTTTGACAG AGACCAGTTT TCACCAGATG 4921 ATTTCCTGGG TCGTACTGAA ATTCCAGTGG CAAAAATTCG AACAGAACAG GAAAGCAAAG 4981 GCCCTATGAC CCGCCGACTG CTGCTGCATG AGGTCCCCAC CGGGGAGGTC TGGGTCCGTT 5041 TTGACCTGCA GCTTTTTGAG CAAAAAACTC TCCTGTTGCC AACTTTCTTG TACAAAGTGG 5101 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 5161 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 5221 ATCCCATTTC GAGTAAGGGA GCATTACGCG TTAAGTCgac aatcaacctc tggattacaa 5281 aatttgtgaa agatt