Construct: ORF TRCN0000481250

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007503.1_s317c1
Derived from:
ccsbBroadEn_00650
DNA Barcode:
AGCAATGTCGGCCTCTATCAGTAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNA11 (2767)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481250
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2767 GNA11 G protein subunit alpha 11 NM_002067.5 100% 100%
2 human 2776 GNAQ G protein subunit alpha q NM_002072.5 78.5% 90.2% (many diffs)
3 human 2776 GNAQ G protein subunit alpha q XM_017014628.2 64.8% 76% (many diffs)
4 mouse 14672 Gna11 guanine nucleotide binding ... NM_010301.4 89.7% 98% (many diffs)
5 mouse 14682 Gnaq guanine nucleotide binding ... NM_008139.5 80.5% 90.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1146
ORF length:
1077
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gactctggag tccatgatgg cgtgttgcct gagcgatgag gtgaaggagt
121ccaagcggat caacgccgag atcgagaagc agctgcggcg ggacaagcgc gacgcccggc
181gcgagctcaa gctgctgctg ctcggcacgg gcgagagcgg gaagagcacg ttcatcaagc
241agatgcgcat catccacggc gccggctact cggaggagga caagcgcggc ttcaccaagc
301tcgtctacca gaacatcttc accgccatgc aggccatgat ccgggccatg gagacgctca
361agatcctcta caagtacgag cagaacaagg ccaatgcgct cctgatccgg gaggtggacg
421tggagaaggt gaccaccttc gagcatcagt acgtcagtgc catcaagacc ctgtgggagg
481acccgggcat ccaggaatgc tacgaccgca ggcgcgagta ccagctctcc gactctgcca
541agtactacct gaccgacgtt gaccgcatcg ccaccttggg ctacctgccc acccagcagg
601acgtgctgcg ggtccgcgtg cccaccaccg gcatcatcga gtaccctttc gacctggaga
661acatcatctt ccggatggtg gatgtggggg gccagcggtc ggagcggagg aagtggatcc
721actgctttga gaacgtgaca tccatcatgt ttctcgtcgc ccTCAGCGAA TACGACCAAG
781TCCTGGTGGA GTCGGACAAC GAGAACCGGA TGGAGGAGAG CAAAGCCCTG TTCCGGACCA
841TCATCACCTA CCCCTGGTTC CAGAACTCCT CCGTCATCCT CTTCCTCAAC AAGAAGGACC
901TGCTGGAGGA CAAGATCCTG TACTCGCACC TGGTGGACTA CTTCCCCGAG TTCGATGGTC
961CCCAGCGGGA CGCCCAGGCG GCGCGGGAGT TCATCCTGAA GATGTTCGTG GACCTGAACC
1021CCGACAGCGA CAAGATCATC TACTCACACT TCACGTGTGC CACCGACACG GAGAACATCC
1081GCTTCGTGTT CGCGGCCGTG AAGGACACCA TCCTGCAGCT CAACCTCAAG GAGTACAACC
1141TGGTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1201TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1261CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGCAATGTC GGCCTCTATC AGTAGACGCG
1321TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)