Construct: ORF TRCN0000481250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007503.1_s317c1
- Derived from:
- ccsbBroadEn_00650
- DNA Barcode:
- AGCAATGTCGGCCTCTATCAGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNA11 (2767)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2767 | GNA11 | G protein subunit alpha 11 | NM_002067.5 | 100% | 100% | |
| 2 | human | 2776 | GNAQ | G protein subunit alpha q | NM_002072.5 | 78.5% | 90.2% | (many diffs) |
| 3 | human | 2776 | GNAQ | G protein subunit alpha q | XM_017014628.2 | 64.8% | 76% | (many diffs) |
| 4 | mouse | 14672 | Gna11 | guanine nucleotide binding ... | NM_010301.4 | 89.7% | 98% | (many diffs) |
| 5 | mouse | 14682 | Gnaq | guanine nucleotide binding ... | NM_008139.5 | 80.5% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1146
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactctggag tccatgatgg cgtgttgcct gagcgatgag gtgaaggagt 121 ccaagcggat caacgccgag atcgagaagc agctgcggcg ggacaagcgc gacgcccggc 181 gcgagctcaa gctgctgctg ctcggcacgg gcgagagcgg gaagagcacg ttcatcaagc 241 agatgcgcat catccacggc gccggctact cggaggagga caagcgcggc ttcaccaagc 301 tcgtctacca gaacatcttc accgccatgc aggccatgat ccgggccatg gagacgctca 361 agatcctcta caagtacgag cagaacaagg ccaatgcgct cctgatccgg gaggtggacg 421 tggagaaggt gaccaccttc gagcatcagt acgtcagtgc catcaagacc ctgtgggagg 481 acccgggcat ccaggaatgc tacgaccgca ggcgcgagta ccagctctcc gactctgcca 541 agtactacct gaccgacgtt gaccgcatcg ccaccttggg ctacctgccc acccagcagg 601 acgtgctgcg ggtccgcgtg cccaccaccg gcatcatcga gtaccctttc gacctggaga 661 acatcatctt ccggatggtg gatgtggggg gccagcggtc ggagcggagg aagtggatcc 721 actgctttga gaacgtgaca tccatcatgt ttctcgtcgc ccTCAGCGAA TACGACCAAG 781 TCCTGGTGGA GTCGGACAAC GAGAACCGGA TGGAGGAGAG CAAAGCCCTG TTCCGGACCA 841 TCATCACCTA CCCCTGGTTC CAGAACTCCT CCGTCATCCT CTTCCTCAAC AAGAAGGACC 901 TGCTGGAGGA CAAGATCCTG TACTCGCACC TGGTGGACTA CTTCCCCGAG TTCGATGGTC 961 CCCAGCGGGA CGCCCAGGCG GCGCGGGAGT TCATCCTGAA GATGTTCGTG GACCTGAACC 1021 CCGACAGCGA CAAGATCATC TACTCACACT TCACGTGTGC CACCGACACG GAGAACATCC 1081 GCTTCGTGTT CGCGGCCGTG AAGGACACCA TCCTGCAGCT CAACCTCAAG GAGTACAACC 1141 TGGTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGCAATGTC GGCCTCTATC AGTAGACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt