Construct: ORF TRCN0000481318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001641.1_s317c1
- Derived from:
- ccsbBroadEn_09273
- DNA Barcode:
- CTTGAACAGTGTGCCTTCGGTTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIGLEC10 (89790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_033130.5 | 99.9% | 99.8% | 144G>A;677C>T |
| 2 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_001171156.2 | 91.5% | 91.5% | 144G>A;418_419ins174;503C>T |
| 3 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | XM_005259366.5 | 86.9% | 86.8% | 144G>A;677C>T;754_755ins270 |
| 4 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_001171157.2 | 86.2% | 86.2% | 144G>A;677C>T;1330_1331ins285 |
| 5 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_001171158.2 | 79.3% | 79.3% | (many diffs) |
| 6 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | XM_005259367.4 | 78.6% | 78.4% | (many diffs) |
| 7 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_001171159.2 | 77.9% | 77.9% | (many diffs) |
| 8 | human | 114132 | SIGLEC11 | sialic acid binding Ig like... | NM_052884.3 | 76.4% | 65.8% | (many diffs) |
| 9 | human | 114132 | SIGLEC11 | sialic acid binding Ig like... | XM_005258476.3 | 74.4% | 64.1% | (many diffs) |
| 10 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_001322105.2 | 73.3% | 73.1% | (many diffs) |
| 11 | human | 114132 | SIGLEC11 | sialic acid binding Ig like... | NM_001135163.1 | 67.8% | 54.9% | (many diffs) |
| 12 | human | 89790 | SIGLEC10 | sialic acid binding Ig like... | NM_001171161.2 | 65% | 64.8% | (many diffs) |
| 13 | human | 400709 | SIGLEC16 | sialic acid binding Ig like... | NM_001348364.2 | 59% | 50.4% | (many diffs) |
| 14 | human | 114132 | SIGLEC11 | sialic acid binding Ig like... | XM_005258477.3 | 55.6% | 50.5% | (many diffs) |
| 15 | human | 114132 | SIGLEC11 | sialic acid binding Ig like... | XR_243888.4 | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2160
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctactgcca ctgctgctgt cctcgctgct gggcgggtcc caggctatgg 121 atgggagatt ctggatacga gtgcaggagt cagtgatggt gccggagggc ctgtgcatct 181 ctgtgccctg ctctttctcc tacccccgac aagactggac agggtctacc ccagcttatg 241 gctactggtt caaagcagtg actgagacaa ccaagggtgc tcctgtggcc acaaaccacc 301 agagtcgaga ggtggaaatg agcacccggg gccgattcca gctcactggg gatcccgcca 361 aggggaactg ctccttggtg atcagagacg cgcagatgca ggatgagtca cagtacttct 421 ttcgggtgga gagaggaagc tatgtgagat ataatttcat gaacgatggg ttctttctaa 481 aagtaacagc cctgactcag aagcctgatg tctacatccc cgagaccctg gagcccgggc 541 agccggtgac ggtcatctgt gtgtttaact gggcctttga ggaatgtcca cccccttctt 601 tctcctggac gggggctgcc ctctcctccc aaggaaccaa accaacgacc tcccacttct 661 cagtgctcag cttcacgccc agaccccagg accacaacac cgacctcacc tgccatgtgg 721 acttctccag aaagggtgtg agcgtacaga ggaccgtccg actccgtgtg gcctatgccc 781 ccagagacct tgttatcagc atttcacgtg acaacacgcc agccctggag ccccagcccc 841 agggaaatgt cccatacctg gaagcccaaa aaggccagtt cctgcggctc ctctgtgctg 901 ctgacagcca gccccctgcc acactgagct gggtcctgca gaacagagtc ctctcctcgt 961 cccatccctg gggccctaga cccctggggc tggagctgcc cggggtgaag gctggggatt 1021 cagggcgcta cacctgccga gcggagaaca ggcttggctc ccagcagcga gccctggacc 1081 tctctgtgca gtatcctcca gagaacctga gagtgatggt ttcccaagca aacaggacag 1141 tcctggaaaa ccttgggaac ggcacgtctc tcccagtact ggagggccaa agcctgtgcc 1201 tggtctgtgt cacacacagc agccccccag ccaggctgag ctggacccag aggggacagg 1261 ttctgagccc ctcccagccc tcagaccccg gggtcctgga gctgcctcgg gttcaagtgg 1321 agcacgaagg agagttcacc tgccacgctc ggcacccact gggctcccag cacgtctctc 1381 tcagcctctc cgtgcactac tccccgaagc tgctgggccc ctcctgctcc tgggaggctg 1441 agggtctgca ctgcagctgc tcctcccagg ccagcccggc cccctctctg cgctggtggc 1501 ttggggagga gctgctggag gggaacagca gccaggactc cttcgaggtc acccccagct 1561 cagccgggcc ctgggccaac agctccctga gcctccatgg agggctcagc tccggcctca 1621 ggctccgctg tgaggcctgg aacgtccatg gggcccagag tggatccatc ctgcagctgc 1681 cagataagaa gggactcatc tcaacggcat tctccaacgg agcgtttctg ggaatcggca 1741 tcacggctct tcttttccTC TGCCTGGCCC TGATCATCAT GAAGATTCTA CCGAAGAGAC 1801 GGACTCAGAC AGAAACCCCG AGGCCCAGGT TCTCCCGGCA CAGCACGATC CTGGATTACA 1861 TCAATGTGGT CCCGACGGCT GGCCCCCTGG CTCAGAAGCG GAATCAGAAA GCCACACCAA 1921 ACAGTCCTCG GACCCCTCTT CCACCAGGTG CTCCCTCCCC AGAATCAAAG AAGAACCAGA 1981 AAAAGCAGTA TCAGTTGCCC AGTTTCCCAG AACCCAAATC ATCCACTCAA GCCCCAGAAT 2041 CCCAGGAGAG CCAAGAGGAG CTCCATTATG CCACGCTCAA CTTCCCAGGC GTCAGACCCA 2101 GGCCTGAGGC CCGGATGCCC AAGGGCACCC AGGCGGATTA TGCAGAAGTC AAGTTCCAAT 2161 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGACTTGA ACAGTGTGCC TTCGGTTGGA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt