Construct: ORF TRCN0000481319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001275.2_s317c1
- Derived from:
- ccsbBroadEn_12358
- DNA Barcode:
- ACTAATCCGTAGCTAAGATCATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRGAP1 (57522)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57522 | SRGAP1 | SLIT-ROBO Rho GTPase activa... | XM_024449097.1 | 83.8% | 83.2% | (many diffs) |
| 2 | human | 57522 | SRGAP1 | SLIT-ROBO Rho GTPase activa... | XM_024449096.1 | 82.2% | 78.8% | (many diffs) |
| 3 | human | 57522 | SRGAP1 | SLIT-ROBO Rho GTPase activa... | XM_011538580.2 | 44.1% | 41.3% | (many diffs) |
| 4 | human | 57522 | SRGAP1 | SLIT-ROBO Rho GTPase activa... | XM_011538581.2 | 44.1% | 41.3% | (many diffs) |
| 5 | human | 57522 | SRGAP1 | SLIT-ROBO Rho GTPase activa... | NM_001346201.2 | 43.1% | 40.1% | (many diffs) |
| 6 | human | 57522 | SRGAP1 | SLIT-ROBO Rho GTPase activa... | NM_020762.4 | 42.5% | 39.8% | (many diffs) |
| 7 | mouse | 117600 | Srgap1 | SLIT-ROBO Rho GTPase activa... | NM_001242411.1 | 38.9% | 39.6% | (many diffs) |
| 8 | mouse | 117600 | Srgap1 | SLIT-ROBO Rho GTPase activa... | XM_006513140.2 | 38.7% | 40.1% | (many diffs) |
| 9 | mouse | 117600 | Srgap1 | SLIT-ROBO Rho GTPase activa... | NM_001081037.2 | 38% | 39.3% | (many diffs) |
| 10 | mouse | 117600 | Srgap1 | SLIT-ROBO Rho GTPase activa... | XR_003948581.1 | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1503
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg agttcagctt ctccaggatc tgcaagattt cttccgaaaa aaagctgaaa 121 ttgagacgga atattcccgg aatctagaga agttagcaga aaggttcatg gcaaaaacaa 181 gaagcactaa ggatcatcaa caatacaaga aagaccagaa cctgttgtct ccagtgaact 241 gctggtattt gctcctgaac caagtaagga gagaaagcaa agaccatgca accttgagtg 301 acatctatct gaacaatgtg attatgcggt tcatgcagat aagtgaggat tctaccagga 361 tgtttaaaaa gagcaaagag attgcattcc aacttcatga ggatttaatg aaggttctta 421 atgagcttta tacggtgatg aaaacatacc atatgtatca tgcagagagc atcagtgcag 481 agagcaagct gaaagaggcc gaaaaacaag aggaaaagca aattgggaga tctggtgatc 541 cagtcttcca tattcgacta gaggagagac atcaacggcg aagctctgta aagaaaattg 601 aaaaaatgaa agaaaaaaga caagcaaaat attcagaaaa taagctaaaa tcaattaagg 661 cacggaacga atatctccta acacttgaag ccaccaatgc ctcagttttc aagtactata 721 ttcatgatct ttctgattta attgattgct gtgatcttgg ctaccatgca agtctgaaca 781 gagccctaag aacatatctg tctgcggagt acaaccttga aacctccaga catgagggct 841 tagacattat tgagaatgca gttgataatt tagagcccag gagcgataag cagagattca 901 tggagatgta ccctgctgcg ttctgtccac caatgaagtt tgagtttcag tctcacatgg 961 gtgatgaggt gtgccaggtc agtgcccagc agccagtcca ggcagagctc atgcTCAGGT 1021 ACCAACAGTT GCAGTCCCGC CTTGCCACGC TCAAAATCGA GAATGAAGAG GTTAAGAAAA 1081 CGACTGAAGC CACCTTGCAG ACGATACAAG ATATGGTCAC CATCGAGGAC TATGATGTTT 1141 CTGAATGCTT CCAGCACAGT CGTTCCACAG AATCTGTGAA GTCCACTGTC TCTGAAACCT 1201 ACCTGAGTAA ACCCAGCATC GCCAAGAGAA GAGCCAACCA GCAGGAAACT GAACAGTTCT 1261 ACTTCATGAA ACTCAGAGAA TATTTGGAAG GCAGTAATCT CATCACAAAA CTTCAAGCCA 1321 AACATGACTT GCTGCAGAGG ACCCTGGGAG AAGGTGAGTT ATCCAAAATG TATGGGAAGA 1381 TGACCTGGAT GATACATCGT ATCATGTATA TATTCCAGAA AGAGTATGAA TTGTATATTT 1441 GTGCTTTAAG AAGCAGAGGA CACTCTGTGT TTAGATTTGG TTTTGTTTGT ATATGTCATG 1501 ATTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TAATCCGTAG CTAAGATCAT CAACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt