Transcript: Human NM_020762.4

Homo sapiens SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
SRGAP1 (57522)
Length:
22920
CDS:
118..3375

Additional Resources:

NCBI RefSeq record:
NM_020762.4
NBCI Gene record:
SRGAP1 (57522)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020762.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047557 CGTCATCCTGACGGCTATTTA pLKO.1 2632 CDS 100% 15.000 21.000 N SRGAP1 n/a
2 TRCN0000289513 CGTCATCCTGACGGCTATTTA pLKO_005 2632 CDS 100% 15.000 21.000 N SRGAP1 n/a
3 TRCN0000308155 GCTAAATTAACACGGGCATTT pLKO_005 3528 3UTR 100% 10.800 15.120 N SRGAP1 n/a
4 TRCN0000047556 CCGCTCACAGTATAATACTAA pLKO.1 1602 CDS 100% 5.625 7.875 N SRGAP1 n/a
5 TRCN0000251714 CCAGTGAACTGCTGGTATTTG pLKO_005 403 CDS 100% 13.200 10.560 N Srgap1 n/a
6 TRCN0000047553 GCCCTGTTTATGAGAAATGTA pLKO.1 2222 CDS 100% 5.625 4.500 N SRGAP1 n/a
7 TRCN0000289514 GCCCTGTTTATGAGAAATGTA pLKO_005 2222 CDS 100% 5.625 4.500 N SRGAP1 n/a
8 TRCN0000047554 GCTGAAATTGAGACGGAATAT pLKO.1 286 CDS 100% 13.200 9.240 N SRGAP1 n/a
9 TRCN0000289515 GCTGAAATTGAGACGGAATAT pLKO_005 286 CDS 100% 13.200 9.240 N SRGAP1 n/a
10 TRCN0000251716 TTGCCCAGGTCGGTCCTTATA pLKO_005 1984 CDS 100% 13.200 9.240 N Srgap1 n/a
11 TRCN0000047555 GCAGAGATTCATGGAGATGTA pLKO.1 1062 CDS 100% 4.950 2.970 N SRGAP1 n/a
12 TRCN0000289516 GCAGAGATTCATGGAGATGTA pLKO_005 1062 CDS 100% 4.950 2.970 N SRGAP1 n/a
13 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 19760 3UTR 100% 4.950 2.475 Y CFLAR n/a
14 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 19760 3UTR 100% 4.950 2.475 Y C19orf31 n/a
15 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 19945 3UTR 100% 4.050 2.025 Y P3H4 n/a
16 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 19945 3UTR 100% 4.050 2.025 Y ORAI2 n/a
17 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 19945 3UTR 100% 4.050 2.025 Y P3H4 n/a
18 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 11379 3UTR 100% 13.200 6.600 Y LIAS n/a
19 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 16150 3UTR 100% 10.800 5.400 Y SMIM11A n/a
20 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5791 3UTR 100% 5.625 2.813 Y KLHL30 n/a
21 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5791 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020762.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12358 pDONR223 100% 42.5% 39.8% None (many diffs) n/a
2 ccsbBroad304_12358 pLX_304 0% 42.5% 39.8% V5 (many diffs) n/a
3 TRCN0000481319 ACTAATCCGTAGCTAAGATCATCA pLX_317 34.2% 42.5% 39.8% V5 (many diffs) n/a
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