Construct: ORF TRCN0000481323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017731.1_s317c1
- Derived from:
- ccsbBroadEn_14034
- DNA Barcode:
- TCTTTGTGCACTTCCTTGTCAAAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABI1 (10006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10006 | ABI1 | abl interactor 1 | NM_001012752.2 | 99.9% | 99.3% | 1419delC |
| 2 | human | 10006 | ABI1 | abl interactor 1 | XM_005252333.4 | 99.7% | 99.1% | 802_803insCAG;1416delC |
| 3 | human | 10006 | ABI1 | abl interactor 1 | NM_001012750.2 | 98.8% | 98.3% | 459_473del;1434delC |
| 4 | human | 10006 | ABI1 | abl interactor 1 | NM_001178119.1 | 98.6% | 98.1% | 459_473del;817_818insCAG;1431delC |
| 5 | human | 10006 | ABI1 | abl interactor 1 | XM_005252332.4 | 94.5% | 94% | 804_884del;1500delC |
| 6 | human | 10006 | ABI1 | abl interactor 1 | NM_001348030.1 | 93.8% | 93.2% | 981_982ins87;1332delC |
| 7 | human | 10006 | ABI1 | abl interactor 1 | NM_001348029.1 | 93.7% | 93% | 459_473del;821_899delinsC;1512delC |
| 8 | human | 10006 | ABI1 | abl interactor 1 | NM_005470.3 | 93.6% | 93.1% | 459_473del;819_899del;1515delC |
| 9 | human | 10006 | ABI1 | abl interactor 1 | NM_001178121.1 | 93.6% | 93% | 802_803insCAG;978_979ins87;1329delC |
| 10 | human | 10006 | ABI1 | abl interactor 1 | NM_001012751.2 | 92.8% | 92.3% | 459_473del;996_997ins87;1347delC |
| 11 | human | 10006 | ABI1 | abl interactor 1 | NM_001178120.1 | 92.6% | 92% | (many diffs) |
| 12 | human | 10006 | ABI1 | abl interactor 1 | NM_001348034.1 | 89.3% | 86.7% | (many diffs) |
| 13 | human | 10006 | ABI1 | abl interactor 1 | NM_001348033.1 | 88% | 87.3% | (many diffs) |
| 14 | human | 10006 | ABI1 | abl interactor 1 | NM_001348032.1 | 87.9% | 87.4% | (many diffs) |
| 15 | human | 10006 | ABI1 | abl interactor 1 | NM_001178122.1 | 86.6% | 85.8% | 459_473del;820_821ins177;1257delC |
| 16 | human | 10006 | ABI1 | abl interactor 1 | NM_001178116.1 | 85% | 84.5% | (many diffs) |
| 17 | human | 10006 | ABI1 | abl interactor 1 | NM_001348031.1 | 84.9% | 81.5% | (many diffs) |
| 18 | human | 10006 | ABI1 | abl interactor 1 | NM_001178124.1 | 81.4% | 80.6% | 805_806ins264;1155delC |
| 19 | human | 10006 | ABI1 | abl interactor 1 | NM_001178123.1 | 80.5% | 79.8% | 459_473del;820_821ins264;1170delC |
| 20 | human | 10006 | ABI1 | abl interactor 1 | NM_001178125.1 | 69% | 68.2% | 283_284ins177;628_629ins264;978delC |
| 21 | human | 10006 | ABI1 | abl interactor 1 | XM_011519289.2 | 60.8% | 57.5% | (many diffs) |
| 22 | human | 10006 | ABI1 | abl interactor 1 | XM_017015459.1 | 57.6% | 54.2% | (many diffs) |
| 23 | human | 10006 | ABI1 | abl interactor 1 | NR_145410.1 | 37.8% | (many diffs) | |
| 24 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_145994.3 | 92.2% | 97.6% | (many diffs) |
| 25 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_007380.4 | 92% | 97.4% | (many diffs) |
| 26 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_001077190.3 | 91.3% | 96.6% | (many diffs) |
| 27 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_001331043.1 | 91.1% | 96.4% | (many diffs) |
| 28 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_001077192.3 | 86.3% | 91.5% | (many diffs) |
| 29 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_001331044.1 | 85.7% | 90.8% | (many diffs) |
| 30 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_001331045.1 | 85.5% | 90.6% | (many diffs) |
| 31 | mouse | 11308 | Abi1 | abl-interactor 1 | NM_001077193.3 | 75% | 79.6% | (many diffs) |
| 32 | mouse | 11308 | Abi1 | abl-interactor 1 | XM_006497619.1 | 74.2% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagctgcag atgttactag aggaggagat cccgtctggc aagagggcgc 121 tgatcgagag ttaccagaac ctgactcggg tggcagacta ctgtgaaaac aactacatac 181 aggctacaga caagagaaaa gctttagagg agaccaaagc ctatacaacc caatctctag 241 ctagtgttgc ttatcaaata aatgcattgg ccaacaatgt actccagttg ctggatatcc 301 aagcctctca gcttcggaga atggagtctt ccatcaatca tatctcacag actgtggata 361 ttcataagga gaaagtggca cgaagagaga ttggtatttt gacaacaaat aagaatacat 421 caagaactca caaaataata gcacctgcga atatggagcg ccctgtaagg tatattcgga 481 aacctatcga ttacacagtt ctggatgatg tgggccatgg tgtcaagcat ggaaataacc 541 agcctgcaag aactggcaca ctgtcgagaa caaatcctcc tactcagaaa ccgccaagtc 601 ctcccatgtc aggccgggga acactgggac ggaatactcc ttataaaacc ctggaacctg 661 ttaaaccccc aacagttcct aatgactata tgaccagtcc tgctaggctt ggaagtcagc 721 atagtccagg caggacagca tctttaaatc agagaccaag gacacacagt ggaagtagtg 781 gaggaagtgg aagtcgagaa aacagtggta gcagtagtat tggcattccc attgctgtgc 841 ctacaccttc gccacccact attggaccag cagccccggg ctcagctcct ggttcccagt 901 atggcacaat gaccaggcag atatctcgac acaactctac tacttcttcg acatcttctg 961 gtggatacag acgaactccc tctgtgactg ctcaattttc tgctcagcct catgttaatg 1021 gaggtccact ttattctcaa aattcaattt ctattgctcc accccctccc cctatgccTC 1081 AGTTGACTCC ACAGATACCT CTCACAGGCT TCGTGGCCAG GGTGCAGGAA AACATTGCTG 1141 ATAGTCCAAC TCCACCGCCA CCACCTCCAC CAGATGACAT TCCCATGTTT GATGACTCTC 1201 CACCTCCCCC ACCACCACCA CCAGTGGATT ATGAAGATGA GGAGGCTGCA GTAGTTCAGT 1261 ATAATGATCC ATATGCAGAT GGGGATCCTG CTTGGGCCCC CAAGAATTAT ATTGAGAAAG 1321 TTGTTGCAAT ATATGATTAT ACAAAAGACA AGGATGATGA GCTGTCATTT ATGGAGGGTG 1381 CAATCATTTA TGTTATAAAG AAGAATGATG ATGGCTGGTA TGAAGGAGTC TGCAATCGAG 1441 TGACTGGTCT GTTCCCTGGG AACTATGTTG AATCAATCAT GCATATACTG ATTACCCAAC 1501 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1561 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1621 ATCTTGTGGA AAGGACGATC TTTGTGCACT TCCTTGTCAA ATACGCGTTA AGTCgacaat 1681 caacctctgg attacaaaat ttgtgaaaga tt