Construct: ORF TRCN0000481354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013302.1_s317c1
- Derived from:
- ccsbBroadEn_05395
- DNA Barcode:
- ACCCGTTTCTTCACCTAGCTGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCAIM (285343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285343 | TCAIM | T cell activation inhibitor... | NM_001282913.2 | 100% | 100% | |
| 2 | human | 285343 | TCAIM | T cell activation inhibitor... | NM_173826.4 | 100% | 100% | |
| 3 | human | 285343 | TCAIM | T cell activation inhibitor... | NM_001282914.2 | 70.9% | 70.9% | 0_1ins432 |
| 4 | human | 285343 | TCAIM | T cell activation inhibitor... | NM_001282915.2 | 70.9% | 70.9% | 0_1ins432 |
| 5 | human | 285343 | TCAIM | T cell activation inhibitor... | XR_001740117.2 | 30.2% | (many diffs) | |
| 6 | human | 285343 | TCAIM | T cell activation inhibitor... | XR_001740118.2 | 27.4% | (many diffs) | |
| 7 | human | 285343 | TCAIM | T cell activation inhibitor... | NM_001029839.2 | 13.3% | 12% | (many diffs) |
| 8 | human | 285343 | TCAIM | T cell activation inhibitor... | NM_001029840.2 | 13.3% | 12% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1557
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttttgccac ctgagaccta tgaggaggtt atgtctagag aagatatttc 121 cacactggtt tcccttttca agagctttat cgggagctga agcagtcaat gccttgaggc 181 ctttctattt tgcagtacat ccagatttct ttggacagca ccccgtagaa agggaaatca 241 atgaaaattc tcttaaaagg ttaagtgtct acctagaaaa cctccagaaa ccaggcttca 301 agtctttgaa accaactcag cttacatttt atgtaagaga aacagaccag agttcctccg 361 atggccagga accttttagt acttccggat ttcgagcagt caaatttact ttgcacacca 421 gagatctgct aagcacagtg ttatatattc tcaactcctg cagtttatct gttgaacata 481 tccaaagctt gaatactaat atgcataccc agcctctcaa agaagctaaa aggatgcctg 541 acaggcccat caaatgggac aagtcttatt actcctttac tggattcaag gaccctgatg 601 aagaccttga acaagtctcg agagtggaaa caactctcac atcctggtta gataacaatg 661 ggaaaagtgc tgttaaaaag ctaaagaaca gtttgccact tagaaaagaa ctagatcgtt 721 taaaagatga actgtctcat caattgcaac tctcagatat caggtggcag aggagctggg 781 gcatcgccca ccgctgtagc cagctgcata gtttaagccg cttagcacag cagaatttgg 841 aaacacttaa aaaagcaaaa gggtgtacaa tcatatttac agaccgttct ggcatgagtg 901 cagtgggcca tgtgatgcta ggaacaatgg atgtccatca ccactggaca aaactttttg 961 aaagattgcc aagttatttt gaccttcaga ggaggctgat gattttagaa gaccaaataa 1021 gctatctttt aggtggCATA CAAGTTGTTT ATATTGAAGA ATTACAGCCA GTATTGACAC 1081 TTGAAGAATA TTACTCTCTT CTTGATGTGT TTTATAATAG ACTGTTGAAA AGTAGAATAC 1141 TATTTCACCC TCGAAGTTTG CGTGGTTTAC AAATGATCCT TAACAGTGAC AGATATGCTC 1201 CAAGCTTGCA TGAACTCGGG CATTTTAATA TTCCAACACT CTGTGATCCA GCAAATCTCC 1261 AGTGGTTTAT TCTCACCAAA GCTCAGCAGG CAAGAGAGAA CATGAAAAGA AAGGAAGAGT 1321 TAAAGGTTAT TGAAAATGAA TTGATACAGG CATCAACAAA GAAATTTTCT TTGGAGAAGT 1381 TATATAAAGA GCCCAGCATT TCTAGTATAC AAATGGTGGA TTGTTGTAAG AGACTTCTAG 1441 AACAATCACT GCCTTACCTA CATGGGATGC ACCTCTGCAT TTCACATTTT TACTCTGTTA 1501 TGCAAGATGG AGACCTTTGT ATTCCTTGGA ATTGGAAGAA TGGAGAAGCC ATTAAGTTGC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GAACCCGTTT CTTCACCTAG CTGTCCACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt