Construct: ORF TRCN0000481364

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006186.1_s317c1
Derived from:
ccsbBroadEn_03984
DNA Barcode:
CACACGAACGTTTACTGCCGCCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC30A5 (64924)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481364
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64924 SLC30A5 solute carrier family 30 me... NM_024055.5 100% 100%
2 human 64924 SLC30A5 solute carrier family 30 me... NM_001251969.2 65.2% 65.2% 82_83ins123
3 human 64924 SLC30A5 solute carrier family 30 me... XM_006714672.4 13.9% 13% (many diffs)
4 human 64924 SLC30A5 solute carrier family 30 me... NM_022902.5 13.2% 12.3% (many diffs)
5 mouse 69048 Slc30a5 solute carrier family 30 (z... NM_022885.2 12.3% 11.1% (many diffs)
6 mouse 69048 Slc30a5 solute carrier family 30 (z... XR_382742.3 8.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
444
ORF length:
354
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAGGAGAA ATACGGCGGG GACGTGCTGG
121CCGGCCCCGG CGGCGGCGGC GGCCTTGGGC CGGTGGACGT ACCCAGCGCT CGATTAACAA
181AATATATTGT GTTACTATGT TTCACTAAAT TTTTGAAGGC TGTGGGACTT TTCGAATCAT
241ATGATCTCCT AAAAGCTGTT CACATTGTTC AGTTCATTTT TATATTAAAA CTTGGGACTG
301CATTTTTTAT GGTTTTGTTT CAAAAGCCAT TTTCTTCTGG GAAAACTATT ACCAAACACC
361AGATAATTGG ATCACTAAAA ATTCCTGGTA GAAAAGAATT TAAAGACAAA AAGTTAAATG
421ATCCTAGGAA ACTAGTGGGA AACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
481AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
541TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACACGAACGT
601TTACTGCCGC CATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
661att

Download FASTA (ORF) (Full)