Construct: ORF TRCN0000481368
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003059.4_s317c1
- Derived from:
- ccsbBroadEn_05930
- DNA Barcode:
- TGGGGGTCGACGCTCATAGTTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK2D (817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481368
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_172115.2 | 99.9% | 100% | 1431C>T |
| 2 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_172127.2 | 99.9% | 100% | 1431C>T |
| 3 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_172128.3 | 99.8% | 99.7% | 1431C>T;1434C>G |
| 4 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008678.2 | 99.7% | 99.7% | 943_944insCAG;1428C>T |
| 5 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321576.2 | 99.6% | 99.5% | 943_944insCAG;1428C>T;1431C>G |
| 6 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_006714330.3 | 98.3% | 98.9% | (many diffs) |
| 7 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_172129.1 | 97.6% | 97.7% | 984_1016del;1464C>T |
| 8 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532291.2 | 97.6% | 97.7% | 984_1016del;1464C>T |
| 9 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321582.2 | 97.6% | 97.5% | 984_1016del;1464C>T;1467C>G |
| 10 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321589.2 | 97% | 97.1% | 982_1023del;1473C>T |
| 11 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_172114.1 | 97% | 97.1% | 982_1023del;1473C>T |
| 12 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321590.2 | 97% | 96.9% | 982_1023del;1473C>T;1476C>G |
| 13 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321584.2 | 96.8% | 96.7% | (many diffs) |
| 14 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001221.4 | 95.7% | 95.7% | 1431C>T;1435_1497del |
| 15 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321583.2 | 95.6% | 96.1% | (many diffs) |
| 16 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321575.2 | 95.5% | 95.9% | (many diffs) |
| 17 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321574.2 | 95.5% | 95.5% | 943_944insCAG;1428C>T;1432_1494del |
| 18 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008677.2 | 95.4% | 95.9% | (many diffs) |
| 19 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321577.2 | 95.3% | 95.7% | (many diffs) |
| 20 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321591.2 | 94.9% | 95% | 982_1056del;1506C>T |
| 21 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321587.2 | 94.8% | 94.8% | 982_1056del;1506C>T;1509C>G |
| 22 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532289.2 | 93.6% | 93.7% | 984_1016del;1464C>T;1468_1530del |
| 23 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532290.2 | 93.4% | 93.5% | (many diffs) |
| 24 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321568.2 | 93.4% | 93.8% | (many diffs) |
| 25 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321579.2 | 93.2% | 93.3% | 982_1083del;1533C>T |
| 26 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321570.2 | 93.2% | 93.1% | 982_1083del;1533C>T;1536C>G |
| 27 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321566.2 | 93.1% | 93.1% | 982_1023del;1473C>T;1477_1539del |
| 28 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321586.2 | 93% | 92.9% | (many diffs) |
| 29 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321588.2 | 92.9% | 92.9% | (many diffs) |
| 30 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532295.2 | 92.2% | 92.7% | (many diffs) |
| 31 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321580.2 | 92% | 92.1% | 984_1043del;1491C>T;1495_1557del |
| 32 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321567.2 | 91.8% | 92.3% | (many diffs) |
| 33 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321572.2 | 91.8% | 92.1% | (many diffs) |
| 34 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008672.2 | 91.6% | 92.2% | (many diffs) |
| 35 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008674.2 | 91.6% | 91.9% | (many diffs) |
| 36 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008673.2 | 91.5% | 92% | (many diffs) |
| 37 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321573.2 | 91.1% | 91.2% | 982_1056del;1506C>T;1510_1572del |
| 38 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321569.2 | 89.6% | 89.6% | 982_1083del;1533C>T;1537_1599del |
| 39 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_005263253.4 | 89.4% | 89.4% | (many diffs) |
| 40 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321571.2 | 88.2% | 88.7% | (many diffs) |
| 41 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_005263254.4 | 88% | 88.5% | (many diffs) |
| 42 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321581.2 | 87.7% | 86.5% | (many diffs) |
| 43 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008675.2 | 81% | 80.1% | (many diffs) |
| 44 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008676.1 | 81% | 80.1% | (many diffs) |
| 45 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532292.2 | 65% | 65.1% | (many diffs) |
| 46 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321578.2 | 57.1% | 57.1% | (many diffs) |
| 47 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321585.2 | 57.1% | 57.1% | (many diffs) |
| 48 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321592.2 | 14% | 12.3% | (many diffs) |
| 49 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_023813.4 | 91% | 99.7% | (many diffs) |
| 50 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319421.1 | 89.7% | 98.9% | (many diffs) |
| 51 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500829.3 | 88.9% | 97.5% | (many diffs) |
| 52 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001293664.1 | 88.4% | 96.9% | (many diffs) |
| 53 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500825.3 | 87.3% | 95.7% | (many diffs) |
| 54 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001025438.2 | 87.2% | 95.7% | (many diffs) |
| 55 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500826.3 | 87% | 95.5% | (many diffs) |
| 56 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319422.1 | 86.1% | 94.9% | (many diffs) |
| 57 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500823.3 | 85.3% | 93.7% | (many diffs) |
| 58 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500824.3 | 85.1% | 93.5% | (many diffs) |
| 59 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001293663.1 | 84.9% | 93.1% | (many diffs) |
| 60 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500820.3 | 84.8% | 93.1% | (many diffs) |
| 61 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319419.1 | 84.7% | 92.9% | (many diffs) |
| 62 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500822.3 | 84.6% | 92.9% | (many diffs) |
| 63 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319420.1 | 84.1% | 92.7% | (many diffs) |
| 64 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500819.3 | 83.8% | 92.1% | (many diffs) |
| 65 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001025439.2 | 83.7% | 92.1% | (many diffs) |
| 66 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319416.1 | 83.6% | 91.9% | (many diffs) |
| 67 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319417.1 | 83.6% | 92.2% | (many diffs) |
| 68 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319418.1 | 83.4% | 92% | (many diffs) |
| 69 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001346635.1 | 81.6% | 89.6% | (many diffs) |
| 70 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500818.3 | 81.4% | 89.4% | (many diffs) |
| 71 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001346636.1 | 80.5% | 88.7% | (many diffs) |
| 72 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_017319415.1 | 80.3% | 88.5% | (many diffs) |
| 73 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500833.3 | 71.9% | 67.5% | (many diffs) |
| 74 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001293666.1 | 68% | 72.3% | (many diffs) |
| 75 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500839.3 | 67.8% | 71.9% | (many diffs) |
| 76 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500835.3 | 65.3% | 69.4% | (many diffs) |
| 77 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500834.3 | 63.3% | 67.1% | (many diffs) |
| 78 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500836.3 | 62.9% | 69.5% | (many diffs) |
| 79 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | NM_001293665.1 | 62.3% | 67.7% | (many diffs) |
| 80 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500831.3 | 58.9% | 65.1% | (many diffs) |
| 81 | mouse | 108058 | Camk2d | calcium/calmodulin-dependen... | XM_006500832.3 | 58.3% | 63.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcgaccaca acctgcacca ggttcacgga cgagtatcag cttttcgagg 121 agcttggaaa gggggcattc tcagtggtga gaagatgtat gaaaattcct actggacaag 181 aatatgctgc caaaattatc aacaccaaaa agctttctgc tagggatcat cagaaactag 241 aaagagaagc tagaatctgc cgtcttttga agcaccctaa tattgtgcga cttcatgata 301 gcatatcaga agagggcttt cactacttgg tgtttgattt agttactgga ggtgaactgt 361 ttgaagacat agtggcaaga gaatactaca gtgaagctga tgccagtcat tgtatacagc 421 agattctaga aagtgttaat cattgtcacc taaatggcat agttcacagg gacctgaagc 481 ctgagaattt gcttttagct agcaaatcca agggagcagc tgtgaaattg gcagactttg 541 gcttagccat agaagttcaa ggggaccagc aggcgtggtt tggttttgct ggcacacctg 601 gatatctttc tccagaagtt ttacgtaaag atccttatgg aaagccagtg gatatgtggg 661 catgtggtgt cattctctat attctacttg tggggtatcc acccttctgg gatgaagacc 721 aacacagact ctatcagcag atcaaggctg gagcttatga ttttccatca ccagaatggg 781 acacggtgac tcctgaagcc aaagacctca tcaataaaat gcttactatc aaccctgcca 841 aacgcatcac agcctcagag gcactgaagc acccatggat ctgtcaacgt tctactgttg 901 cttccatgat gcacagacag gagactgtag actgcttgaa gaaatttaat gctagaagaa 961 aactaaaggg tgccatcttg acaactatgc tggctacaag gaatttctca gcagccaaga 1021 gtttgttgaa gaaaccagat ggagtaaagg agtcaactga gagttcaaat acaaCAATTG 1081 AGGATGAAGA TGTGAAAGCA CGAAAGCAAG AGATTATCAA AGTCACTGAA CAACTGATCG 1141 AAGCTATCAA CAATGGGGAC TTTGAAGCCT ACACAAAAAT CTGTGACCCA GGCCTTACTG 1201 CTTTTGAACC TGAAGCTTTG GGTAATTTAG TGGAAGGGAT GGATTTTCAC CGATTCTACT 1261 TTGAAAATGC TTTGTCCAAA AGCAATAAAC CAATCCACAC TATTATTCTA AACCCTCATG 1321 TACATCTGGT AGGGGATGAT GCCGCCTGCA TAGCATATAT TAGGCTCACA CAGTACATGG 1381 ATGGCAGTGG AATGCCAAAG ACAATGCAGT CAGAAGAGAC TCGTGTGTGG CACCGCCGGG 1441 ATGGAAAGTG GCAGAATGTT CATTTTCATC GCTCGGGGTC ACCAACAGTA CCCATTAAGT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGATGGGG GTCGACGCTC ATAGTTAATA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt