Transcript: Human NM_001321589.2

Homo sapiens calcium/calmodulin dependent protein kinase II delta (CAMK2D), transcript variant 30, mRNA.

Source:
NCBI, updated 2019-09-01
Taxon:
Homo sapiens (human)
Gene:
CAMK2D (817)
Length:
5673
CDS:
671..2149

Additional Resources:

NCBI RefSeq record:
NM_001321589.2
NBCI Gene record:
CAMK2D (817)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146878 AGAATATAGAGAATGACACC pXPR_003 TGG 603 41% 9 1.0328 CAMK2D CAMK2D 77186
2 BRDN0001146856 TATCTTCCCAGAGTTACTGG pXPR_003 AGG 281 19% 5 0.1458 CAMK2D CAMK2D 77187
3 BRDN0001146112 AAAAAGCTTTCTGCTAGGGG pXPR_003 TGG 158 11% 2 -1.0671 CAMK2D CAMK2D 77185
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321589.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434219 GTTAATCATTGTCACCTAAAT pLKO_005 1040 CDS 100% 13.200 18.480 N CAMK2D n/a
2 TRCN0000195041 CCTGAAGCTTTGGGTAATTTA pLKO.1 1856 CDS 100% 15.000 10.500 N CAMK2D n/a
3 TRCN0000000475 TGTGGTGTCATTCTCTATATT pLKO.1 1268 CDS 100% 15.000 10.500 N CAMK2D n/a
4 TRCN0000422299 TGATCGAAGCTATCAACAATG pLKO_005 1782 CDS 100% 10.800 7.560 N CAMK2D n/a
5 TRCN0000195698 CCAAAGACAATGCAGTCAGAA pLKO.1 2042 CDS 100% 4.950 3.465 N CAMK2D n/a
6 TRCN0000195462 CCCTAATATTGTGCGACTTCA pLKO.1 880 CDS 100% 4.950 3.465 N CAMK2D n/a
7 TRCN0000000472 GCAATAAACCAATCCACACTA pLKO.1 1929 CDS 100% 4.950 3.465 N CAMK2D n/a
8 TRCN0000196967 GTCACCAACAGTACCCATCAA pLKO.1 2125 CDS 100% 4.950 3.465 N CAMK2D n/a
9 TRCN0000195619 CCATGGATCTGTCAACGTTCT pLKO.1 1478 CDS 100% 4.050 2.835 N CAMK2D n/a
10 TRCN0000000473 CTTACTATCAACCCTGCCAAA pLKO.1 1427 CDS 100% 4.050 2.835 N CAMK2D n/a
11 TRCN0000000471 CTTCTGCATTCTCTGTTCTCA pLKO.1 2209 3UTR 100% 3.000 2.100 N CAMK2D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321589.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14565 pDONR223 0% 97.1% 97.1% None 982_1023del n/a
2 ccsbBroad304_14565 pLX_304 0% 97.1% 97.1% V5 982_1023del n/a
3 TRCN0000481152 TAATACCTAGCTTTCCAGTGCCAA pLX_317 31.4% 97.1% 97.1% V5 982_1023del n/a
4 ccsbBroadEn_05930 pDONR223 100% 97% 97.1% None 982_1023del;1473C>T n/a
5 ccsbBroad304_05930 pLX_304 0% 97% 97.1% V5 982_1023del;1473C>T n/a
6 TRCN0000481368 TGGGGGTCGACGCTCATAGTTAAT pLX_317 29.9% 97% 97.1% V5 982_1023del;1473C>T n/a
7 ccsbBroadEn_14562 pDONR223 100% 71.3% 42.6% None (many diffs) n/a
8 ccsbBroad304_14562 pLX_304 0% 71.3% 42.6% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000474396 TAATTGTTATTCACCCAAGAGACT pLX_317 23.8% 71.3% 42.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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