Construct: ORF TRCN0000481419

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018878.3_s317c1
Derived from:
ccsbBroadEn_14915
DNA Barcode:
ATCCCTCTAAATTAGTAATAGAAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
KSR1 (8844)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481419
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8844 KSR1 kinase suppressor of ras 1 NM_014238.2 98.7% 61.8% (many diffs)
2 human 8844 KSR1 kinase suppressor of ras 1 XM_017025271.1 95.7% 59.9% (many diffs)
3 human 8844 KSR1 kinase suppressor of ras 1 XM_017025272.1 95.7% 59.9% (many diffs)
4 human 8844 KSR1 kinase suppressor of ras 1 XM_017025273.1 95.7% 59.9% (many diffs)
5 human 8844 KSR1 kinase suppressor of ras 1 XM_017025274.2 95.7% 59.9% (many diffs)
6 human 8844 KSR1 kinase suppressor of ras 1 XM_017025275.2 95.7% 59.9% (many diffs)
7 human 8844 KSR1 kinase suppressor of ras 1 XM_017025276.1 95.7% 59.9% (many diffs)
8 human 8844 KSR1 kinase suppressor of ras 1 XM_017025270.2 93.5% 58.5% (many diffs)
9 human 8844 KSR1 kinase suppressor of ras 1 XM_011525430.2 83.7% 52.3% (many diffs)
10 human 8844 KSR1 kinase suppressor of ras 1 XM_011525431.2 83.5% 52.4% (many diffs)
11 human 8844 KSR1 kinase suppressor of ras 1 XM_011525429.2 81.5% 51% (many diffs)
12 human 8844 KSR1 kinase suppressor of ras 1 XM_017025267.1 80% 49.5% (many diffs)
13 human 8844 KSR1 kinase suppressor of ras 1 NM_001367810.1 79.1% 48.5% (many diffs)
14 human 8844 KSR1 kinase suppressor of ras 1 XM_006722154.3 77.6% 47% (many diffs)
15 human 8844 KSR1 kinase suppressor of ras 1 XR_934587.3 72.3% (many diffs)
16 human 8844 KSR1 kinase suppressor of ras 1 XM_017025277.1 72.2% 35.8% (many diffs)
17 human 8844 KSR1 kinase suppressor of ras 1 XM_017025278.1 70.4% 34.7% (many diffs)
18 human 8844 KSR1 kinase suppressor of ras 1 XR_934588.3 70.3% (many diffs)
19 human 8844 KSR1 kinase suppressor of ras 1 XM_017025279.1 42.8% 49.7% (many diffs)
20 human 8844 KSR1 kinase suppressor of ras 1 XM_017025280.2 40.8% 48.7% (many diffs)
21 human 8844 KSR1 kinase suppressor of ras 1 XR_002958082.1 34.5% (many diffs)
22 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_011248772.2 77.5% 48.1% (many diffs)
23 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_011248775.2 77.5% 48.1% (many diffs)
24 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_011248774.3 75.5% 46.9% (many diffs)
25 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_011248773.3 72.7% 45.2% (many diffs)
26 mouse 16706 Ksr1 kinase suppressor of ras 1 NM_001348207.1 71.2% 43.5% (many diffs)
27 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_006532335.4 70.3% 43.6% (many diffs)
28 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_006532334.4 68.3% 42.5% (many diffs)
29 mouse 16706 Ksr1 kinase suppressor of ras 1 NM_013571.3 66.9% 40.9% (many diffs)
30 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_011248770.3 66.5% 41.3% (many diffs)
31 mouse 16706 Ksr1 kinase suppressor of ras 1 XM_011248771.3 65% 39.8% (many diffs)
32 mouse 16706 Ksr1 kinase suppressor of ras 1 XR_388355.4 57.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1599
ORF length:
1530
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaatgaggcc aaggtgaagg agacgctgcg gcgctgtggg gccagcgggg
121atgagtgtgg ccgtctgcag tatgccctca cctgcctgcg gaaggtgaca ggcctgggag
181gggagcacaa ggaggactcc agttggagtt cattggatgc gcggcgggaa agtggctcag
241ggccttccac ggacaccctc tcagcagcca gcctgccctg gcccccaggg agctcccagc
301tgggcagagc aggcaacagc gtccagggcc cacgctccat ctccgtgtca gctctgcccg
361cctcagactc ccccaccccc agcttcagtg agggcctctc agacacctgt attcccctgc
421acgccagcgg ccggctgacc ccccgtgccc tgcacagctt catcaccccg cccaccacac
481cccagctgcg acggcacacc aagctgaagc caccacggac gcccccccca cccagccgca
541aggtcttcca gctgctgccc agcttcccca cactcacccg gagcaagtcc catgagtctc
601agctggggaa ccgcattgat gacgtctcct cgatgaggtt tgatctctcg catggatccc
661cacagatggt acggagggat atcgggctgt cggtgacgca caggttctcc accaagtcct
721ggctgtcgca gtctgccacg tgtgccagag agcatgatat ttggagtgaa gtgcaagcat
781tgcagttgaa gtgtcacaac aaatgtacaa agagcccctg ctgtagatat ctctgcacta
841actcggcttc gaggacagaa tctgtcccct cggacatcac caacccggtg gacagagcag
901ccgaacccca ttttggaacc ctccccaaag cactgacaaa gaaggagcac cctccggcca
961tgaatcacct ggactccagc agcaaccctt cctccaccac ctcctccaca ccctcctcac
1021cggcgccctt cccgacatca tccaacccat ccagcgccac cacgcccccc aacccctcac
1081ctggccagcg ggacagcagg ttcaacttcc cagctgccta cttcattcat catagacagc
1141agtttatctt tccagtgcca tctgctggcc attgctggaa atgcctcctt attgcagaaa
1201gtttaaagga aaacgctttc aacatttcag cctttgcaca cgcagccccg ctccctgaag
1261ctgccgacgg tacccggctc gatgaccagc cgaaagcaga tgtgttggaa gctcacgaag
1321cggaggctga ggagccagag gctggcaagt cagaggcaga agacgatgag gacgaggtgg
1381acgacttgcc gagctctcgc cggccctggc ggggccccat ctctcgcaag gccagccaga
1441ccagcgtgta cctgcaggag tgggacatcc ccttcgagca ggtagagctg ggcgagccca
1501tcgggcaggg ccgctggggc cgggtgcacc gcggccgctg gcatggcgag tggcccatcg
1561cctgcttgaa gatggacggc ccacaaccca gacccacctg aagctctcan ngaaagaggt
1621gatgaactac cggcagacgc ggcatgagaa cgtggtgctc ttcatggggg cctgcatgaa
1681cccgccccac cctggccatt atcaccagct tctgcaaggg gcggacgttg cactcgtttg
1741tgagggaccc caagacgtct ctggacatca acaagacgag gcaaatcgct caggagatca
1801tcaagggcat gggatatctt catgccaagg gcatcgtaca caaagatctc aaatctaaga
1861acgtcttcta tgacaacggc aaggtggtca tcacagactt cgggctgttt gggatcTCAG
1921GCGTGGTCCG AGAGGGACGG CGTGAGAACC AGCTAAAGCT GTCCCACGAC TGGCTGTGCT
1981ATCTGGCCCC TGAGATTGTA CGCGAGATGA CCCCCGGGAA GGACGAGGAT CAGCTGCCAT
2041TCTCCAAAGC TGCTGATGTC TATGCATTTG GGACTGTTTG GTATGAGCTG CAAGCAAGAG
2101ACTGGCCCTT GAAGAACCAG GCTGCAGAGG CATCCATCTG GCAGATTGGA AGCGGGGAAG
2161GAATGAAGCG TGTCCTGACT TCTGTCAGCT TGGGGAAGGA AGTCAGTGAG ATCCTGTCGG
2221CCTGCTGGGC TTTCGACCTG CAGGAGAGAC CCAGCTTCAG CCTGCTGATG GACATGCTGG
2281AGAAACTTCC CAAGCTGAAC CGGCGGCTCT CCCACCCTGG ACACTTCTGG AAGTCAGCTG
2341AGTTGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2401TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2461CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCCCTCTA AATTAGTAAT AGAAAACGCG
2521TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)