Construct: ORF TRCN0000481446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012543.1_s317c1
- Derived from:
- ccsbBroadEn_07136
- DNA Barcode:
- CGCACGCACCATTTCGTCACGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNT9A (7483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7483 | WNT9A | Wnt family member 9A | NM_003395.4 | 99.9% | 100% | 153T>C |
| 2 | human | 7483 | WNT9A | Wnt family member 9A | XM_011544271.2 | 80.8% | 80.8% | 0_1ins210 |
| 3 | mouse | 216795 | Wnt9a | wingless-type MMTV integrat... | NM_139298.2 | 89.1% | 98% | (many diffs) |
| 4 | mouse | 216795 | Wnt9a | wingless-type MMTV integrat... | XM_006532855.2 | 88% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1164
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggatggg tccccgctgg cgcgctggct ggccgcggcc ttcgggctga 121 cgctgctgct cgccgcgctg cgcccttcgg ccgcctactt cgggctgacg ggcagcgagc 181 ccctgaccat cctcccgctg accctggagc cagaggcggc cgcccaggcg cactacaagg 241 cctgcgaccg gctgaagctg gagcggaagc agcggcgcat gtgccgccgg gacccgggcg 301 tggcagagac gctggtggag gccgtgagca tgagtgcgct cgagtgccag ttccagttcc 361 gctttgagcg ctggaactgc acgctggagg gccgctaccg ggccagcctg ctcaagcgag 421 gcttcaagga gactgccttc ctctatgcca tctcctcggc tggcctgacg cacgcactgg 481 ccaaggcgtg cagcgcgggc cgcatggagc gctgtacctg cgatgaggca cccgacctgg 541 agaaccgtga ggcctggcag tgggggggct gcggagacaa ccttaagtac agcagcaagt 601 tcgtcaagga attcctgggc agacggtcaa gcaaggatct gcgagcccgt gtggacttcc 661 acaacaacct cgtgggtgtg aaggtgatca aggctggggt ggagaccacc tgcaagtgcc 721 acggcgtgtc aggctcatgc acggtgcgga cctgctggcG GCAGTTGGCG CCTTTCCATG 781 AGGTGGGCAA GCATCTGAAG CACAAGTATG AGACGGCACT CAAGGTGGGC AGCACCACCA 841 ATGAAGCTGC CGGCGAGGCA GGTGCCATCT CCCCACCACG GGGCCGTGCC TCGGGGGCAG 901 GTGGCAGCGA CCCGCTGCCC CGCACTCCAG AGCTGGTGCA CCTGGATGAC TCGCCTAGCT 961 TCTGCCTGGC TGGCCGCTTC TCCCCGGGCA CCGCTGGCCG TAGGTGCCAC CGTGAGAAGA 1021 ACTGCGAGAG CATCTGCTGT GGCCGCGGCC ATAACACACA GAGCCGGGTG GTGACAAGGC 1081 CCTGCCAGTG CCAGGTGCGT TGGTGCTGCT ATGTGGAGTG CAGGCAGTGC ACGCAGCGTG 1141 AGGAGGTCTA CACCTGCAAG GGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCACGCACCA 1321 TTTCGTCACG GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att