Construct: ORF TRCN0000481482

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014745.1_s317c1
Derived from:
ccsbBroadEn_00517
DNA Barcode:
CTTTAGAGGTAACGCATGAACATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ESR1 (2099)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481482
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2099 ESR1 estrogen receptor 1 NM_000125.3 100% 100%
2 human 2099 ESR1 estrogen receptor 1 NM_001122740.1 100% 100%
3 human 2099 ESR1 estrogen receptor 1 NM_001122741.1 100% 100%
4 human 2099 ESR1 estrogen receptor 1 NM_001122742.1 100% 100%
5 human 2099 ESR1 estrogen receptor 1 XM_011535543.2 99.8% 100% 729T>C;975G>C
6 human 2099 ESR1 estrogen receptor 1 XM_011535544.2 99.8% 100% 729T>C;975G>C
7 human 2099 ESR1 estrogen receptor 1 XM_011535545.2 99.8% 100% 729T>C;975G>C
8 human 2099 ESR1 estrogen receptor 1 XM_017010376.1 99.8% 100% 729T>C;975G>C
9 human 2099 ESR1 estrogen receptor 1 XM_017010377.1 99.8% 100% 729T>C;975G>C
10 human 2099 ESR1 estrogen receptor 1 XM_017010378.1 99.8% 100% 729T>C;975G>C
11 human 2099 ESR1 estrogen receptor 1 XM_017010379.1 99.8% 100% 729T>C;975G>C
12 human 2099 ESR1 estrogen receptor 1 XM_017010380.1 99.8% 100% 729T>C;975G>C
13 human 2099 ESR1 estrogen receptor 1 XM_017010381.1 99.8% 100% 729T>C;975G>C
14 human 2099 ESR1 estrogen receptor 1 NM_001291241.1 99.8% 99.8% 756_757insGGT
15 human 2099 ESR1 estrogen receptor 1 NM_001291230.1 99.6% 99.6% 644_649delGTAATA
16 human 2099 ESR1 estrogen receptor 1 XM_011535547.2 79.5% 76.4% (many diffs)
17 human 2099 ESR1 estrogen receptor 1 XM_006715374.3 78.2% 76.8% (many diffs)
18 human 2099 ESR1 estrogen receptor 1 XM_006715375.3 70.8% 70.9% 0_1ins519;210T>C;456G>C
19 human 2099 ESR1 estrogen receptor 1 XR_002956266.1 65.5% (many diffs)
20 human 2099 ESR1 estrogen receptor 1 XM_017010382.2 63% 63.1% 0_1ins657;72T>C;318G>C
21 human 2099 ESR1 estrogen receptor 1 XM_011535549.2 58% 57.1% (many diffs)
22 human 2099 ESR1 estrogen receptor 1 XM_017010383.1 55.7% 55.7% 0_1ins789;186G>C
23 human 2099 ESR1 estrogen receptor 1 XR_001743223.2 52.9% (many diffs)
24 human 2099 ESR1 estrogen receptor 1 NM_001328100.2 50.6% 47.9% (many diffs)
25 mouse 13982 Esr1 estrogen receptor 1 (alpha) NM_001302531.1 85.6% 88.9% (many diffs)
26 mouse 13982 Esr1 estrogen receptor 1 (alpha) NM_001302532.1 85.6% 88.9% (many diffs)
27 mouse 13982 Esr1 estrogen receptor 1 (alpha) NM_007956.5 85.6% 88.9% (many diffs)
28 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_006512433.3 85.6% 88.9% (many diffs)
29 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_006512434.3 85.6% 88.9% (many diffs)
30 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_006512435.2 85.6% 88.9% (many diffs)
31 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_011243066.2 85.6% 88.9% (many diffs)
32 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_011243067.2 85.6% 88.9% (many diffs)
33 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_011243068.2 85.6% 88.9% (many diffs)
34 mouse 13982 Esr1 estrogen receptor 1 (alpha) XM_017313797.1 54.2% 54.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1854
ORF length:
1785
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaccatgacc ctccacacca aagcatctgg gatggcccta ctgcatcaga
121tccaagggaa cgagctggag cccctgaacc gtccgcagct caagatcccc ctggagcggc
181ccctgggcga ggtgtacctg gacagcagca agcccgccgt gtacaactac cccgagggcg
241ccgcctacga gttcaacgcc gcggccgccg ccaacgcgca ggtctacggt cagaccggcc
301tcccctacgg ccccgggtct gaggctgcgg cgttcggctc caacggcctg gggggtttcc
361ccccactcaa cagcgtgtct ccgagcccgc tgatgctact gcacccgccg ccgcagctgt
421cgcctttcct gcagccccac ggccagcagg tgccctacta cctggagaac gagcccagcg
481gctacacggt gcgcgaggcc ggcccgccgg cattctacag gccaaattca gataatcgac
541gccagggtgg cagagaaaga ttggccagta ccaatgacaa gggaagtatg gctatggaat
601ctgccaagga gactcgctac tgtgcagtgt gcaatgacta tgcttcaggc taccattatg
661gagtctggtc ctgtgagggc tgcaaggcct tcttcaagag aagtattcaa ggacataacg
721actatatgtg tccagccacc aaccagtgca ccattgataa aaacaggagg aagagctgcc
781aggcctgccg gctccgcaaa tgctacgaag tgggaatgat gaaaggtggg atacgaaaag
841accgaagagg agggagaatg ttgaaacaca agcgccagag agatgatggg gagggcaggg
901gtgaagtggg gtctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga
961tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg
1021ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc agacccttca
1081gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga
1141tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc
1201ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc
1261acccagggaa gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg
1321tagagggcat ggtggagatc TTCGACATGC TGCTGGCTAC ATCATCTCGG TTCCGCATGA
1381TGAATCTGCA GGGAGAGGAG TTTGTGTGCC TCAAATCTAT TATTTTGCTT AATTCTGGAG
1441TGTACACATT TCTGTCCAGC ACCCTGAAGT CTCTGGAAGA GAAGGACCAT ATCCACCGAG
1501TCCTGGACAA GATCACAGAC ACTTTGATCC ACCTGATGGC CAAGGCAGGC CTGACCCTGC
1561AGCAGCAGCA CCAGCGGCTG GCCCAGCTCC TCCTCATCCT CTCCCACATC AGGCACATGA
1621GTAACAAAGG CATGGAGCAT CTGTACAGCA TGAAGTGCAA GAACGTGGTG CCCCTCTATG
1681ACCTGCTGCT GGAGATGCTG GACGCCCACC GCCTACATGC GCCCACTAGC CGTGGAGGGG
1741CATCCGTGGA GGAGACGGAC CAAAGCCACT TGGCCACTGC GGGCTCTACT TCATCGCATT
1801CCTTGCAAAA GTATTACATC ACGGGGGAGG CAGAGGGTTT CCCTGCCACG GTCTTGCCAA
1861CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1921ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1981TATCTTGTGG AAAGGACGAC TTTAGAGGTA ACGCATGAAC ATCACGCGTT AAGTCgacaa
2041tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)