Construct: ORF TRCN0000481549

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008696.1_s317c1
Derived from:
ccsbBroadEn_07091
DNA Barcode:
GATCAAGGGTCTCTCCCCTACTAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPM1 (7168)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481549
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7168 TPM1 tropomyosin 1 NM_001018008.2 99.8% 100% 345C>A
2 human 7168 TPM1 tropomyosin 1 NM_001330351.2 95.5% 93.4% (many diffs)
3 human 7168 TPM1 tropomyosin 1 NM_001330346.2 94% 92.7% (many diffs)
4 human 7168 TPM1 tropomyosin 1 NM_001365782.1 94% 92.7% (many diffs)
5 human 7168 TPM1 tropomyosin 1 NM_001301289.2 93% 91.1% (many diffs)
6 human 7168 TPM1 tropomyosin 1 NM_001365780.1 90.8% 83.8% (many diffs)
7 human 7168 TPM1 tropomyosin 1 NM_001365781.2 89.8% 86.2% (many diffs)
8 human 7168 TPM1 tropomyosin 1 XM_005254650.3 89.8% 86.2% (many diffs)
9 human 7168 TPM1 tropomyosin 1 NM_001330344.2 88.9% 84.6% (many diffs)
10 human 7168 TPM1 tropomyosin 1 XM_017022540.1 84.1% 78.8% (many diffs)
11 human 7168 TPM1 tropomyosin 1 NM_001365776.1 80.8% 75.6% (many diffs)
12 human 7168 TPM1 tropomyosin 1 XM_005254646.2 80.4% 73.2% (many diffs)
13 human 7168 TPM1 tropomyosin 1 XM_017022539.2 77.3% 70% (many diffs)
14 human 7168 TPM1 tropomyosin 1 NM_001301244.2 76.9% 68.9% (many diffs)
15 human 7168 TPM1 tropomyosin 1 NM_001018007.2 76.2% 67.5% (many diffs)
16 human 7168 TPM1 tropomyosin 1 NM_001018005.2 76.1% 69.6% (many diffs)
17 human 7168 TPM1 tropomyosin 1 NM_001365779.1 76.1% 69.6% (many diffs)
18 human 7168 TPM1 tropomyosin 1 NM_001018004.2 75.4% 68.2% (many diffs)
19 human 7168 TPM1 tropomyosin 1 NM_001365777.1 73.7% 63.3% (many diffs)
20 human 7168 TPM1 tropomyosin 1 NM_001018020.2 72.8% 62% (many diffs)
21 human 7168 TPM1 tropomyosin 1 NM_000366.6 72.7% 64.1% (many diffs)
22 human 7168 TPM1 tropomyosin 1 XM_006720667.4 72.7% 64.1% (many diffs)
23 human 7168 TPM1 tropomyosin 1 XM_017022538.2 72.7% 64.1% (many diffs)
24 human 7168 TPM1 tropomyosin 1 NM_001018006.2 72% 62.7% (many diffs)
25 human 7168 TPM1 tropomyosin 1 XM_005254639.4 70.5% 58.1% (many diffs)
26 human 7168 TPM1 tropomyosin 1 XM_017022535.2 69.8% 59.5% (many diffs)
27 human 7168 TPM1 tropomyosin 1 XM_017022534.2 69.1% 60.1% (many diffs)
28 human 7168 TPM1 tropomyosin 1 NM_001365778.1 67.7% 58.2% (many diffs)
29 human 7168 TPM1 tropomyosin 1 XM_017022537.2 66.8% 54.6% (many diffs)
30 human 7168 TPM1 tropomyosin 1 XM_017022536.2 66% 55.2% (many diffs)
31 human 7168 TPM1 tropomyosin 1 XM_024450040.1 48.5% 41.5% (many diffs)
32 human 7168 TPM1 tropomyosin 1 XR_002957675.1 27.4% (many diffs)
33 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164256.1 93.4% 98.3% (many diffs)
34 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511051.3 88.9% 91.8% (many diffs)
35 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164254.1 88.5% 91.5% (many diffs)
36 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164252.1 86.9% 89.9% (many diffs)
37 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511049.3 86.9% 88.4% (many diffs)
38 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511050.2 84.1% 85% (many diffs)
39 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164253.1 82.7% 83.4% (many diffs)
40 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242716.2 82.6% 82% (many diffs)
41 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511045.2 78.1% 74.2% (many diffs)
42 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164255.1 76.9% 74.9% (many diffs)
43 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511046.2 74.4% 68.6% (many diffs)
44 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511041.1 74.1% 68.6% (many diffs)
45 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511042.3 74.1% 68.6% (many diffs)
46 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511039.3 73% 66.2% (many diffs)
47 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164249.1 73% 67.2% (many diffs)
48 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164248.1 72.9% 69.3% (many diffs)
49 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511040.3 71.8% 66.8% (many diffs)
50 mouse 22003 Tpm1 tropomyosin 1, alpha NM_024427.4 71.8% 67.9% (many diffs)
51 mouse 22003 Tpm1 tropomyosin 1, alpha XR_379412.3 45% (many diffs)
52 mouse 22003 Tpm1 tropomyosin 1, alpha XR_379413.1 34.1% (many diffs)
53 mouse 22003 Tpm1 tropomyosin 1, alpha XR_001778851.1 21.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
801
ORF length:
735
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggggagtagc tcgctggagg cggtgcgcag gaagatccgg agcctgcagg
121agcaggcgga cgccgctgag gagcgcgcgg gcaccctgca gcgcgagctg gaccacgaga
181ggaagctgag ggagaccgct gaagccgacg tagcttctct gaacagacgc atccagctgg
241ttgaggaaga gttggatcgt gcccaggagc gtctggcaac agctttgcag aagctggagg
301aagctgagaa ggcagcagat gagagtgaga gaggCATGAA AGTCATTGAG AGTCGAGCCC
361AAAAAGATGA AGAAAAAATG GAAATTCAGG AGATCCAACT GAAAGAGGCA AAGCACATTG
421CTGAAGATGC CGACCGCAAA TATGAAGAGG TGGCCCGTAA GCTGGTCATC ATTGAGAGCG
481ACCTGGAACG TGCAGAGGAG CGGGCTGAGC TCTCAGAAGG CAAATGTGCC GAGCTTGAAG
541AAGAATTGAA AACTGTGACG AACAACTTGA AGTCACTGGA GGCTCAGGCT GAGAAGTACT
601CGCAGAAGGA AGACAGATAT GAGGAAGAGA TCAAGGTCCT TTCCGACAAG CTGAAGGAGG
661CTGAGACTCG GGCTGAGTTT GCGGAGAGGT CAGTAACTAA ATTGGAGAAA AGCATTGATG
721ACTTAGAAGA TCAACTCTAC CAGCAACTTG AGCAAAATCG CCGCCTCACT AATGAACTAA
781AGCTGGCCCT GAATGAGGAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
841CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
901AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGATC AAGGGTCTCT
961CCCCTACTAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)