Construct: ORF TRCN0000481607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001059.1_s317c1
- Derived from:
- ccsbBroadEn_07050
- DNA Barcode:
- GAGGAAGGTATGTCTCAAGAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TFAP2A (7020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481607
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7020 | TFAP2A | transcription factor AP-2 a... | NM_001032280.3 | 99.9% | 100% | 1239C>T |
| 2 | human | 7020 | TFAP2A | transcription factor AP-2 a... | NM_001042425.2 | 97.4% | 97.6% | (many diffs) |
| 3 | human | 7020 | TFAP2A | transcription factor AP-2 a... | NM_001372066.1 | 97.4% | 96.5% | (many diffs) |
| 4 | human | 7020 | TFAP2A | transcription factor AP-2 a... | XM_011514833.2 | 87.3% | 84.4% | (many diffs) |
| 5 | human | 7020 | TFAP2A | transcription factor AP-2 a... | XM_006715175.2 | 87.2% | 87.2% | (many diffs) |
| 6 | human | 7020 | TFAP2A | transcription factor AP-2 a... | XM_017011232.1 | 82.3% | 81.5% | (many diffs) |
| 7 | mouse | 21418 | Tfap2a | transcription factor AP-2, ... | NM_001122948.2 | 94.3% | 98.6% | (many diffs) |
| 8 | mouse | 21418 | Tfap2a | transcription factor AP-2, ... | XM_006516903.3 | 92.5% | 96.7% | (many diffs) |
| 9 | mouse | 21418 | Tfap2a | transcription factor AP-2, ... | NM_001301674.1 | 92.3% | 95.6% | (many diffs) |
| 10 | mouse | 21418 | Tfap2a | transcription factor AP-2, ... | NM_011547.4 | 92.3% | 95.6% | (many diffs) |
| 11 | mouse | 21418 | Tfap2a | transcription factor AP-2, ... | XM_006516902.3 | 92% | 96.3% | (many diffs) |
| 12 | mouse | 21418 | Tfap2a | transcription factor AP-2, ... | XM_017315479.1 | 86.7% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt agttcacagt ttttcagcca tggaccgtca cgacggcacc agcaacggga 121 cggcacggtt gccccagctg ggcactgtag gtcaatctcc ctacacgagc gccccgccgc 181 tgtcccacac ccccaatgcc gacttccagc ccccatactt ccccccaccc taccagccta 241 tctaccccca gtcgcaagat ccttactccc acgtcaacga cccctacagc ctgaaccccc 301 tgcacgccca gccgcagccg cagcacccag gctggcccgg ccagaggcag agccaggagt 361 ctgggctcct gcacacgcac cgggggctgc ctcaccagct gtcgggcctg gatcctcgca 421 gggactacag gcggcacgag gacctcctgc acggcccaca cgcgctcagc tcaggactcg 481 gagacctctc gatccactcc ttacctcacg ccatcgagga ggtcccgcat gtagaagacc 541 cgggtattaa catcccagat caaactgtaa ttaagaaagg ccccgtgtcc ctgtccaagt 601 ccaacagcaa tgccgtctcc gccatcccta ttaacaagga caacctcttc ggcggcgtgg 661 tgaaccccaa cgaagtcttc tgttcagttc cgggtcgcct ctcgctcctc agctccacct 721 cgaagtacaa ggtcacggtg gcggaagtgc agcggcggct ctcaccaccc gagtgtctca 781 acgcgtcgct gctgggcgga gtgctccgga gggcgaagtc taaaaatgga ggaagatctt 841 taagagaaaa actggacaaa ataggattaa atctgcctGC AGGGAGACGT AAAGCTGCCA 901 ACGTTACCCT GCTCACATCA CTAGTAGAGG GAGAAGCTGT CCACCTAGCC AGGGACTTTG 961 GGTACGTGTG CGAAACCGAA TTTCCTGCCA AAGCAGTAGC TGAATTTCTC AACCGACAAC 1021 ATTCCGATCC CAATGAGCAA GTGACAAGAA AAAACATGCT CCTGGCTACA AAACAGATAT 1081 GCAAAGAGTT CACCGACCTG CTGGCTCAGG ACCGATCTCC CCTGGGGAAC TCACGGCCCA 1141 ACCCCATCCT GGAGCCCGGC ATCCAGAGCT GCTTGACCCA CTTCAACCTC ATCTCCCACG 1201 GCTTCGGCAG CCCCGCGGTG TGTGCCGCGG TCACGGCCCT GCAGAACTAT CTCACCGAGG 1261 CCCTCAAGGC CATGGACAAA ATGTACCTCA GCAACAACCC CAATAGCCAC ACGGACAACA 1321 ACGCCAAAAG CAGTGACAAA GAGGAGAAGC ACAGAAAGTG CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGAGAGGAA GGTATGTCTC AAGAGCCTAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt