Construct: ORF TRCN0000481617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010294.1_s317c1
- Derived from:
- ccsbBroadEn_08490
- DNA Barcode:
- CACACCCCACCATCCCGGGATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGI2 (55203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55203 | LGI2 | leucine rich repeat LGI fam... | NM_018176.4 | 99.9% | 100% | 1518C>T |
| 2 | human | 55203 | LGI2 | leucine rich repeat LGI fam... | XM_011513850.2 | 57.5% | 48.2% | (many diffs) |
| 3 | human | 55203 | LGI2 | leucine rich repeat LGI fam... | XM_017008356.1 | 51.9% | 49.7% | (many diffs) |
| 4 | mouse | 246316 | Lgi2 | leucine-rich repeat LGI fam... | NM_001310604.1 | 90% | 97.6% | (many diffs) |
| 5 | mouse | 246316 | Lgi2 | leucine-rich repeat LGI fam... | NM_144945.3 | 81.5% | 88% | (many diffs) |
| 6 | mouse | 246316 | Lgi2 | leucine-rich repeat LGI fam... | XM_006503965.3 | 54% | 58.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1704
- ORF length:
- 1635
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgctgcgg agaggcggct gcggagcgct cgggctgctg ctgctgctgc 121 tgggcgccgc gtgcctgata ccgcggagcg cgcaggtgag gcggctggcg cgctgccccg 181 ccacttgcag ctgtaccaag gagtctatca tctgcgtggg ctcttcctgg gtgcccagga 241 tcgtgccggg cgacatcagc tccctgagcc tggtaaatgg gacgttttca gaaatcaagg 301 accgaatgtt ttcccatctg ccttctctgc agctgctatt gctgaattct aactcattca 361 cgatcatccg ggatgatgct tttgctggac tttttcatct tgaatacctg ttcattgaag 421 ggaacaaaat agaaaccatt tcaagaaatg cctttcgtgg cctccgtgac ctgactcacc 481 tttctttggc caataaccac ataaaagcac taccaaggga tgtcttcagt gatttagact 541 ctctgattga actagatttg aggggtaata aatttgaatg tgactgcaaa gccaagtggc 601 tatacctgtg gttgaagatg acaaattcca ccgtttctga tgtgctgtgt attggtccac 661 cagagtatca ggaaaagaag ctaaatgacg tgaccagctt tgactatgaa tgcacaacta 721 cagattttgt tgttcatcag actttaccct accagtcggt ttcagtggat acgttcaact 781 ccaagaacga tgtgtacgtg gccatcgcgc agcccagcat ggagaactgc atggtgctgg 841 agtgggacca cattgaaatg aatttccgga gctatgacaa cattacaggt cagtccatcg 901 tgggctgtaa ggccattctc atcgatgatc aggtctttgt ggtggtagcc cagctcttcg 961 gtggctctca catttacaaa tacgacgaga gttggaccaa atttgtcaaa ttccaagaca 1021 tagaggtctc tcgcatttcc aagcccaatg acatcgagct gtttcagatc gacgacgaga 1081 cgttctttgt catcgcagac agctcaaagg ctggtctgtc cacagtttat aaatggaaca 1141 gcaaaggatt ctattcttac cagtcactgc acgagtggtt cagggacacg gatgcggagt 1201 ttgttgatat cgatggaaaa tcgcatctca tcctgtccag ccgctcccag gtccccatca 1261 TCCTCCAGTG GAATAAAAGC TCTAAGAAGT TTGTCCCCCA TGGTGACATC CCCAACATGG 1321 AGGACGTACT GGCTGTGAAG AGCTTCCGAA TGCAAAATAC CCTCTACCTT TCCCTTACCC 1381 GCTTCATCGG GGACTCCCGG GTCATGAGGT GGAACAGTAA GCAGTTTGTG GAGATCCAAG 1441 CTCTTCCATC CCGGGGGGCC ATGACCCTGC AGCCCTTTTC TTTTAAAGAT AATCACTACC 1501 TGGCCCTGGG GAGTGACTAT ACATTCTCTC AGATATACCA GTGGGATAAA GAGAAGCAGC 1561 TATTCAAAAA GTTTAAGGAG ATTTATGTGC AGGCGCCTCG TTCATTCACA GCTGTCTCCA 1621 CCGACAGGAG AGATTTCTTT TTTGCATCCA GTTTCAAAGG GAAAACAAAG ATTTTTGAAC 1681 ATATAATTGT TGACTTAAGT TTGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1741 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1801 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACACCCCACC 1861 ATCCCGGGAT TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1921 att