Construct: ORF TRCN0000481619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008596.1_s317c1
- Derived from:
- ccsbBroadEn_00981
- DNA Barcode:
- TTCGGATGTTAAATAACATACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MBP (4155)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4155 | MBP | myelin basic protein | NM_001025100.2 | 100% | 100% | |
| 2 | human | 4155 | MBP | myelin basic protein | XM_017025780.2 | 100% | 100% | |
| 3 | human | 4155 | MBP | myelin basic protein | XM_017025778.2 | 65.9% | 67.6% | 1_172del;221_222ins88 |
| 4 | human | 4155 | MBP | myelin basic protein | NM_001025101.2 | 64.4% | 63.4% | (many diffs) |
| 5 | human | 4155 | MBP | myelin basic protein | XM_024451185.1 | 47.7% | 47% | (many diffs) |
| 6 | human | 4155 | MBP | myelin basic protein | XM_024451186.1 | 47.7% | 47% | (many diffs) |
| 7 | human | 4155 | MBP | myelin basic protein | XM_024451187.1 | 47.7% | 47% | (many diffs) |
| 8 | human | 4155 | MBP | myelin basic protein | NM_001025092.2 | 21.5% | 20.4% | (many diffs) |
| 9 | human | 4155 | MBP | myelin basic protein | NM_001025090.2 | 20.7% | 19.7% | (many diffs) |
| 10 | human | 4155 | MBP | myelin basic protein | NM_002385.3 | 19.6% | 18.8% | (many diffs) |
| 11 | human | 4155 | MBP | myelin basic protein | NM_001025081.2 | 18.9% | 18.1% | (many diffs) |
| 12 | human | 4155 | MBP | myelin basic protein | XR_001753202.1 | 17.2% | (many diffs) | |
| 13 | human | 4155 | MBP | myelin basic protein | XR_001753201.1 | 17% | (many diffs) | |
| 14 | human | 4155 | MBP | myelin basic protein | XM_024451188.1 | 15.2% | 14.6% | (many diffs) |
| 15 | mouse | 17196 | Mbp | myelin basic protein | NM_001025245.1 | 87.5% | 83.7% | (many diffs) |
| 16 | mouse | 17196 | Mbp | myelin basic protein | NM_010777.3 | 68.4% | 64.2% | (many diffs) |
| 17 | mouse | 17196 | Mbp | myelin basic protein | XM_006526458.2 | 65.6% | 61.5% | (many diffs) |
| 18 | mouse | 17196 | Mbp | myelin basic protein | XM_006526457.2 | 59.1% | 56% | (many diffs) |
| 19 | mouse | 17196 | Mbp | myelin basic protein | XM_006526456.2 | 56.7% | 53.2% | (many diffs) |
| 20 | mouse | 17196 | Mbp | myelin basic protein | XM_006526455.1 | 55% | 52% | (many diffs) |
| 21 | mouse | 17196 | Mbp | myelin basic protein | XM_006526454.2 | 52.9% | 49.6% | (many diffs) |
| 22 | mouse | 17196 | Mbp | myelin basic protein | XM_006526453.2 | 51.8% | 49% | (many diffs) |
| 23 | mouse | 17196 | Mbp | myelin basic protein | XM_006526452.3 | 48.6% | 46% | (many diffs) |
| 24 | mouse | 17196 | Mbp | myelin basic protein | NM_001025259.2 | 22.7% | 21.6% | (many diffs) |
| 25 | mouse | 17196 | Mbp | myelin basic protein | NM_001025258.2 | 20.4% | 19.4% | (many diffs) |
| 26 | mouse | 17196 | Mbp | myelin basic protein | NM_001025256.2 | 20.3% | 19.7% | (many diffs) |
| 27 | mouse | 17196 | Mbp | myelin basic protein | NM_001025255.2 | 19.7% | 18.7% | (many diffs) |
| 28 | mouse | 17196 | Mbp | myelin basic protein | NM_001025254.2 | 18.4% | 17.8% | (many diffs) |
| 29 | mouse | 17196 | Mbp | myelin basic protein | NM_001025251.2 | 17.8% | 17.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 657
- ORF length:
- 591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaaccacgca ggcaaacgag aattaaatgc cgagaaggcc agtacgaata 121 gtgaaactaa cagaggagaa tctgaaaaaa agagaaacct gggtgaactt tcacggacaa 181 cctcagagga caacgaagtg ttcGGAGAGG CAGATGCGAA CCAGAACAAT GGGACCTCCT 241 CTCAGGACAC AGCGGTGACT GACTCCAAGC GCACAGCGGA CCCGAAGAAT GCCTGGCAGG 301 ATGCCCACCC AGCTGACCCA GGGAGCCGCC CCCACTTGAT CCGCCTCTTT TCCCGAGATG 361 CCCCGGGGAG GGAGGACAAC ACCTTCAAAG ACAGGCCCTC TGAGTCCGAC GAGCTCCAGA 421 CCATCCAAGA AGACAGTGCA GCCACCTCCG AGAGCCTGGA TGTGATGGCG TCACAGAAGA 481 GACCCTCCCA GAGGCACGGA TCCAAGTACC TGGCCACAGC AAGTACCATG GACCATGCCA 541 GGCATGGCTT CCTCCCAAGG CACAGAGACA CGGGCATCCT TGACTCCATC GGGCGCTTCT 601 TTGGCGGTGA CAGGGGTGCG CCCAAGCGGG GCTCTGGCAA GGTGAGCTCT GAGGAGTACC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GATTCGGATG TTAAATAACA TACGCAACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt