Construct: ORF TRCN0000487698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020194.1_s317c1
- DNA Barcode:
- GCGCTTTTCCTGCACCTTGACTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K2 (5605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487698
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_006722799.2 | 99.8% | 100% | 660C>A |
| 2 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026991.1 | 78.8% | 74.2% | (many diffs) |
| 3 | human | 5605 | MAP2K2 | mitogen-activated protein k... | NM_030662.3 | 76.6% | 76.7% | 660C>A;706_984del |
| 4 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026990.1 | 69.8% | 60.7% | (many diffs) |
| 5 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026989.1 | 57.2% | 50% | (many diffs) |
| 6 | human | 407835 | LOC407835 | mitogen-activated protein k... | NR_002144.1 | 51% | (many diffs) | |
| 7 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_001347144.1 | 67.6% | 72.5% | (many diffs) |
| 8 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_023138.5 | 67.4% | 72.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 993
- ORF length:
- 921
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctggcc cggaggaagc cggtgctgcc ggcgctcacc atcaacccta 121 ccatcgccga gggcccatcc cctaccagcg agggcgcctc cgaggcaaac ctggtggacc 181 tgcagaagaa gctggaggag ctggaacttg acgagcagca gaagaagcgg ctggaagcct 241 ttctcaccca gaaagccaag gtcggcgaac tcaaagacga tgacttcgaa aggatctcag 301 agctgggcgc gggcaacggc ggggtggtca ccaaagtcca gcacagaccc tcgggcctca 361 tcatggccag gaagctgatc caccttgaga tcaagccggc catccggaac cagatcatcc 421 gcgagctgca ggtcctgcac gaatgcaact cgccgtacat cgtgggcttc tacggggcct 481 tctacagtga cggggagatc agcatttgca tggaacacat ggacggcggc tccctggacc 541 aggtgctgaa agaggccaag aggattcccg aggagatcct ggggaaagtc agcatcgcgg 601 ttctccgggg cttggcgtac ctccgagaga agcaccagat catgcaccga gatgtgaagc 661 cctccaacat cctcgtgaac tctagagggg agatcaagct gtgtgacttc ggggtgagcg 721 gccagctcat agactccatg gccaactcct tcgtgggcac gcgctcctac atggctccac 781 ctccTAAGCT GCCCAACGGT GTGTTCACCC CCGACTTCCA GGAGTTTGTC AATAAATGCC 841 TCATCAAGAA CCCAGCGGAG CGGGCGGACC TGAAGATGCT CACAAACCAC ACCTTCATCA 901 AGCGGTCCGA GGTGGAAGAA GTGGATTTTG CCGGCTGGTT GTGTAAAACC CTGCGGCTGA 961 ACCAGCCCGG CACACCCACG CGCACCGCCG TGTGAGACCC AGCTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AGCGCTTTTC CTGCACCTTG ACTTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt