Construct: ORF TRCN0000487725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020641.1_s317c1
- DNA Barcode:
- GTTAGATCCCATAGCCACAACTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MINK1 (50488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_001024937.4 | 100% | 100% | |
| 2 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_153827.5 | 98.4% | 98.4% | 1739_1798del |
| 3 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_011523906.1 | 98.2% | 98.2% | 939_940insGGTGAGAAA;1730_1789del |
| 4 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024705.1 | 97.8% | 97.8% | 1347_1433del |
| 5 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721531.2 | 96.3% | 96.3% | 1347_1433del;1826_1885del |
| 6 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721532.2 | 96.3% | 96.3% | 1347_1433del;1826_1885del;2650_2651insGCA |
| 7 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024704.1 | 96.1% | 96.1% | 939_940insGGTGAGAAA;1338_1424del;1817_1876del |
| 8 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_015716.5 | 95.7% | 95.7% | 1739_1798del;2085_2086ins111 |
| 9 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_170663.5 | 95.1% | 95.1% | 1739_1798del;2085_2086ins111;2290_2313del |
| 10 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721536.2 | 93.6% | 93.6% | 1347_1433del;1826_1885del;2172_2173ins111 |
| 11 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024706.1 | 93.6% | 93.6% | (many diffs) |
| 12 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024707.2 | 92.3% | 92.3% | 0_1ins165;1182_1268del;1661_1720del |
| 13 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_001321236.2 | 91.5% | 91.5% | 0_1ins165;1574_1633del;1920_1921ins111 |
| 14 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024708.1 | 91.1% | 91.1% | 0_1ins213;1134_1220del;1613_1672del |
| 15 | human | 50488 | MINK1 | misshapen like kinase 1 | XR_001752522.2 | 76.9% | (many diffs) | |
| 16 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_001045959.1 | 89.3% | 95.6% | (many diffs) |
| 17 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533681.3 | 88.7% | 95% | (many diffs) |
| 18 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_001045964.1 | 88.7% | 95% | (many diffs) |
| 19 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533679.2 | 87.4% | 93.6% | (many diffs) |
| 20 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533677.2 | 86.9% | 93% | (many diffs) |
| 21 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533678.2 | 86.8% | 93% | (many diffs) |
| 22 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_016713.2 | 86.6% | 92.8% | (many diffs) |
| 23 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_176893.2 | 86.1% | 92.3% | (many diffs) |
| 24 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_017314646.1 | 84.8% | 90.9% | (many diffs) |
| 25 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533682.3 | 84.3% | 90.3% | (many diffs) |
| 26 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_017314645.1 | 84.2% | 90.3% | (many diffs) |
| 27 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XR_388490.3 | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4008
- ORF length:
- 3936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcgac ccagcccccg cccgcagcct ggacgacatc gacctgtccg 121 ccctgcggga ccctgctggg atctttgagc ttgtggaggt ggtcggcaat ggaacctacg 181 gacaggtgta caagggtcgg catgtcaaga cggggcagct ggctgccatc aaggtcatgg 241 atgtcacgga ggacgaggag gaagagatca aacaggagat caacatgctg aaaaagtact 301 ctcaccaccg caacatcgcc acctactacg gagccttcat caagaagagc cccccgggaa 361 acgatgacca gctctggctg gtgatggagt tctgtggtgc tggttcagtg actgacctgg 421 taaagaacac aaaaggcaac gccctgaagg aggactgtat cgcctatatc tgcagggaga 481 tcctcagggg tctggcccat ctccatgccc acaaggtgat ccatcgagac atcaaggggc 541 agaatgtgct gctgacagag aatgctgagg tcaagctagt ggattttggg gtgagtgctc 601 agctggaccg caccgtgggc agacggaaca ctttcattgg gactccctac tggatggctc 661 cagaggtcat cgcctgtgat gagaaccctg atgccaccta tgattacagg agtgatattt 721 ggtctctagg aatcacagcc atcgagatgg cagagggagc cccccctctg tgtgacatgc 781 accccatgcg agccctcttc ctcattcctc ggaaccctcc gcccaggctc aagtccaaga 841 agtggtctaa gaagttcatt gacttcattg acacatgtct catcaagact tacctgagcc 901 gcccacccac ggagcagcta ctgaagtttc ccttcatccg ggaccagccc acggagcggc 961 aggtccgcat ccagcttaag gaccacattg accgatcccg gaagaagcgg ggtgagaaag 1021 aggagacaga atatgagtac agcggcagcg aggaggaaga tgacagccat ggagaggaag 1081 gagagccaag ctccatcatg aacgtgcctg gagagtcgac tctacgccgg gagtttctcc 1141 ggctccagca ggaaaataag agcaactcag aggctttaaa acagcagcag cagctgcagc 1201 agcagcagca gcgagacccc gaggcacaca tcaaacacct gctgcaccag cggcagcggc 1261 gcatagagga gcagaaggag gagcggcgcc gcgtggagga gcaacagcgg cgggagcggg 1321 agcagcggaa gctgcaggag aaggagcagc agcggcggct ggaggacatg caggctctgc 1381 ggcgggagga ggagcggcgg caggcggagc gcgagcagga atacaagcgg aagcagctgg 1441 aggagcagcg gcagtcagaa cgtctccaga ggcagctgca gcaggagcat gcctacctca 1501 agtccctgca gcagcagcaa cagcagcagc agcttcagaa acagcagcag cagcagctcc 1561 tgcctgggga caggaagccc ctgtaccatt atggtcgggg catgaatccc gctgacaaac 1621 cagcctgggc ccgagaggta gaagagagaa caaggatgaa caagcagcag aactctccct 1681 tggccaagag caagccaggc agcacggggc ctgagccccc catcccccag gcctccccag 1741 ggcccccagg acccctttcc cagactcctc ctatgcagag gccggtggag ccccaggagg 1801 gaccgcacaa gtccctgcag gaccagccca cccgaaacct ggctgccttc ccagcctccc 1861 atgaccccga ccctgccatc cccgcaccca ctgccacgcc cagtgcccga ggagctgtca 1921 tccgccagaa ttcagacccc acctctgaag gacctggccc cagcccgaat cccccagcct 1981 gggtccgccc agataacgag gccccaccca aggtgcctca gaggacctca tctatcgcca 2041 ctgcccttaa caccagtggg gccggagggt cccggccagc ccaggcagtc cgtgccagac 2101 ctcgcagcaa ctccgcctgg caaatctatc tgcaaaggcg ggcagagcgg ggcaccccaa 2161 agcctccagg gccccctgct cagccccctg gcccgcccaa cgcctctagt aaccccgacc 2221 tcaggaggag cgaccctggc tgggaacgct cggacagcgt ccttccagcc tctcacgggc 2281 acctccccca ggctggctca ctggagcgga accgcgtggg agtctcctcc aaaccggaca 2341 gctcccctgt gctctcccct gggaataaag ccaagcccga cgaccaccgc tcacggccag 2401 gccggcccgc agactttgtg ttgctgaaag agcggactct ggacgaggcc cctcggcctc 2461 ccaagaaggc catggactac tcgtcgtcca gcgaggaggt ggaaagcagt gaggacgacg 2521 aggaggaagg cgaaggcggg ccagcagagg ggagcagaga tacccctggg ggccgcagcg 2581 atggggatac agacagcgtc agcaccatgg tggtccacga cgtcgaggag atcaccggga 2641 cccagccccc atacgggggc ggcaccatgg tggtccagcg cacccctgaa gaggagcgga 2701 acctgctgca tgctgacagc aatgggtaca caaacctgcc tgacgtggtc cagcccagcc 2761 actcacccac cgagaacagc aaaggccaaa gcccaccctc gaaggatggg agtggtgact 2821 accagtctcg tgggctggta aaggcccctg gcaagagctc gttcacgatg tttgtggatc 2881 tagggatcta ccagcctgga ggcagtgggg acagcatccc catcacagcc ctagtgggtg 2941 gagagggcac tcggctcgac cagctgcagt acgacgtgag gaagggttct gtggtcaacg 3001 tgaatcccac caacacccgg gcccacagtg agacccctga gatccggaag tacaagaagc 3061 gattcaactc cgagatcctc tgtgcagccc tttggggggt caacctgctg gtgggcacgg 3121 agaacgggct gatgttgctg gaccgaagtg ggcagggcaa ggtgtatgga ctcattgggc 3181 ggcgacgctt ccagcagatg gatgtgctgg aggggctcaa cctgctcatc accatctcag 3241 ggaaaaggaa caaactgcgg gtgtattacc tgtcctggct ccggaacaag attctgcaca 3301 atgacccaga agtggagaag aagcagggct ggaccaccgt gggggacatg gagggctgcg 3361 ggcactaccg tgttgtgaaa tacgagcgga ttaagttcct ggtcatcgcc ctcaagagct 3421 ccgtggaggt gtatgcctgg gcccccaaac cctaccacaa attcatggcc ttcaagtcct 3481 ttgccgacct cccccaccgc cctctgctgg tcgacctgac agtagaggag gggcagcggc 3541 tcaaggtcat ctatggctcc agtgctggct tccatgctgt ggatgtcgac tcggggaaca 3601 gctatgacat ctacatccct gtgcacatcc agagccagat cacgccccat gccatcatct 3661 tcctccccaa caccgacggc atggagatgc tgctgtgcta cgaggacgag ggtgtctacg 3721 tcaacacgta cgggcgcatc attaaggatg tggtgctgca gtggggggag atgcctactt 3781 ctgtggccta catctgctcc aaccagataa tgggctgggg tgagaaagcc attgagatcc 3841 gctctgtgga gacgggccac ctcgacgggg tcttcatgca caaacgagct cagaggctca 3901 agttcctgtg tgagcggaat gacaaggtgt tttttgcctc agtccgctct gggggcagca 3961 gccaagttta cttcatgaCT CTGAACCGTA ACTGCATCAT GAACTGGTGA GACCCAGCTT 4021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 4081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 4141 CTTGTGGAAA GGACGAGTTA GATCCCATAG CCACAACTTC ACGCGTTAAG TCgacaatca 4201 acctctggat tacaaaattt gtgaaagatt