Construct: ORF TRCN0000487744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020151.1_s317c1
- DNA Barcode:
- TTCGACTTTACGATACGGTGATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DGUOK (1716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318859.2 | 48.6% | 26.8% | 143_255del;381_549del |
| 2 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080918.3 | 47% | 26% | 143_255del;381_567del |
| 3 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134896.2 | 39.2% | 1_31del;299_680del | |
| 4 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134893.2 | 38.2% | 1_31del;299_698del | |
| 5 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_011532647.2 | 32.8% | 18.6% | 143_255del;381_813del |
| 6 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134898.2 | 32.8% | 1_31del;299_814del | |
| 7 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080916.3 | 32.1% | 18.3% | 143_255del;381_831del |
| 8 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134894.2 | 31.5% | 1_31del;299_846del | |
| 9 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134897.2 | 30% | 1_31del;173_258del;385_890del | |
| 10 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_001738656.1 | 26.8% | 1_84del;227_339del;465_995del | |
| 11 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_244926.3 | 26% | 1_83del;226_338del;464_1026del | |
| 12 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318862.2 | 12.7% | 1.1% | 0_1ins178;90_522del |
| 13 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318863.2 | 12.7% | 1.1% | 0_1ins178;90_522del |
| 14 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318860.2 | 12.3% | 12.7% | 0_1ins178;90_540del |
| 15 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318861.2 | 12.3% | 12.7% | 0_1ins178;90_540del |
| 16 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_024452739.1 | 12.3% | 12.7% | 0_1ins178;90_540del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 339
- ORF length:
- 267
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgcg ggccgcctct ttctaagtcg gcttcgagca cccttcagtt 121 ccatggccaa gagcccactc gagggcgttt ccTCCTCCAG AGGCCTGCAC GCGGGGCGCG 181 GGCCCCGAAG GCTCTCCATC GAAGGCAACA TTGGCCTGCA CTGCCCAAAG TCTTGGAAAC 241 TTGCTGGATA TGATGTACCG GGAGCCAGCA CGATGGTCCT ACACATTCCA GACATTTTCC 301 TTTTTGAGCC GCCTGAAAGT ACAGCTGGAG CCCTTCCCTG AGACCCAGCT TTCTTGTACA 361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 481 AGGACGATTC GACTTTACGA TACGGTGATC CACGCGTTAA GTCgacaatc aacctctgga 541 ttacaaaatt tgtgaaagat t