Construct: ORF TRCN0000487766

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF022007.1_s317c1
DNA Barcode:
CATCTTTACATCGCTATTCACAGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CHRFAM7A (89832)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487766
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... NM_139320.1 99.6% 100% (many diffs)
2 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... XM_011522153.2 99.6% 100% (many diffs)
3 human 1139 CHRNA7 cholinergic receptor nicoti... XM_011521177.2 95.6% 93.9% (many diffs)
4 human 1139 CHRNA7 cholinergic receptor nicoti... XM_017021883.2 89.2% 84.8% (many diffs)
5 human 1139 CHRNA7 cholinergic receptor nicoti... XM_017021882.1 89% 84.6% (many diffs)
6 human 1139 CHRNA7 cholinergic receptor nicoti... XM_011521176.3 82.7% 79.4% (many diffs)
7 human 1139 CHRNA7 cholinergic receptor nicoti... NM_000746.6 80% 78% (many diffs)
8 human 1139 CHRNA7 cholinergic receptor nicoti... XM_017021884.1 77.6% 77.9% (many diffs)
9 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... NM_148911.1 77.5% 77.9% (many diffs)
10 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... XM_005254750.3 77.5% 77.9% (many diffs)
11 human 1139 CHRNA7 cholinergic receptor nicoti... NM_001190455.3 75.7% 73.8% (many diffs)
12 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... XM_011522155.3 70.1% 55.5% (many diffs)
13 human 1139 CHRNA7 cholinergic receptor nicoti... XM_011521178.3 55.9% 41.7% (many diffs)
14 human 1139 CHRNA7 cholinergic receptor nicoti... NR_046324.1 36.4% (many diffs)
15 mouse 11441 Chrna7 cholinergic receptor, nicot... NM_007390.3 70.2% 72.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1305
ORF length:
1236
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gcaaaaatat tgcatctacc agcattttca gttccaattg ctaatccagc
121atttgtggat agctgcaaac tgcgatattg ctgatgagcg ctttgacgcc acattccaca
181ctaacgtgtt ggtgaattct tctgggcatt gccagtacct gcctccaggc atattcaaga
241gttcctgcta catcgatgta cgctggtttc cctttgatgt gcagcactgc aaactgaagt
301ttgggtcctg gtcttacgga ggctggtcct tggatctgca gatgcaggag gcagatatca
361gtggctatat ccccaatgga gaatgggacc tagtgggaat ccccggcaag aggagtgaaa
421ggttctatga gtgctgcaaa gagccctacc ccgatgtcac cttcacagtg accatgcgcc
481gcaggacact ctactatggc ctcaacctgc tgatcccctg tgtgctcatc tccgccctcg
541ccctgctggt gttcctgctt cctgcagatt ccggggagaa gatttccctg gggataacag
601tcttactctc tcttaccgtc ttcatgctgc tcgtggctga gatcatgccc gcaacatccg
661attcggtacc attgatagcc cagtacttcg ccagcaccat gatcatcgtg ggcctctcgg
721tggtggtgac ggtgatcgtg ctgcagtacc accaccacga ccccgacggg ggcaagatgc
781ccaagtggac cagagtcatc cttctgaact ggtgcgcgtg gttcctgcga atgaagaggc
841ccggggagga caaggtgcgc ccggcctgcc agcacaagca gcggcgctgc agcctggcca
901gtgtggagat gagcgccgtg gcgccgccgc ccgccagcaa cgggaacctg ctgtacatcg
961gcttccgcgg cctggacggc gtgcactgtg tcccgacccc cgactctggg gtagtgtgtg
1021gccgcatggc ctgctccccc acgcacgatg agcaccTCCT GCACGGTGGG CAACCCCCCG
1081AGGGGGACCC GGACTTGGCC AAGATCCTGG AGGAGGTCCG CTACATTGCC AACCGCTTCC
1141GCTGCCAGGA CGAAAGCGAG GCGGTCTGCA GCGAGTGGAA GTTCGCCGCC TGTGTGGTGG
1201ACCGCCTGTG CCTCATGGCC TTCTCGGTCT TCACCATCAT CTGCACCATC GGCATCCTGA
1261TGTCGGCTCC CAACTTCGTG GAGGCCGTGT CCAAAGACTT TGCGTAAGAC CCAGCTTTCT
1321TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1381CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1441GTGGAAAGGA CGACATCTTT ACATCGCTAT TCACAGAACG CGTTAAGTCg acaatcaacc
1501tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)