Construct: ORF TRCN0000487786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020291.1_s317c1
- DNA Barcode:
- CATGCCTTCATGGACACGATCTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FIGN (55137)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55137 | FIGN | fidgetin, microtubule sever... | NM_001321825.2 | 85.3% | 85.3% | 1_327del;2244_2245insTTG |
| 2 | human | 55137 | FIGN | fidgetin, microtubule sever... | NM_018086.4 | 84% | 84% | 1_360del;2277_2278insTTG |
| 3 | human | 55137 | FIGN | fidgetin, microtubule sever... | XM_011511389.2 | 81.2% | 81.2% | 1_438del;2355_2356insTTG |
| 4 | human | 55137 | FIGN | fidgetin, microtubule sever... | XM_017004419.1 | 81.2% | 81.2% | 1_438del;2355_2356insTTG |
| 5 | mouse | 60344 | Fign | fidgetin | NM_001267846.1 | 78.9% | 83.3% | (many diffs) |
| 6 | mouse | 60344 | Fign | fidgetin | NM_001267847.1 | 78.9% | 83.3% | (many diffs) |
| 7 | mouse | 60344 | Fign | fidgetin | NM_021716.5 | 77.8% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1992
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaactgt gttccggatg ttatcactgc cagcaaagct ggagtcagtt 121 cagccctccc tccagcagat gtctctgcga gtataggaag ctctcctggg gtagccagca 181 acctgacaga acctagttat tcaagtagta cctgtggaag ccacactgta cccagtcttc 241 atgcagggct cccatctcag gaatatgccc caggatacaa cggatcatat ttgcattcta 301 cttatagtag ccagccagca cctgcacttc cttcacctca tccgtctcct ttgcatagct 361 ctgggctact acagccccca ccaccacctc ctccgccacc agccttggtc ccaggctaca 421 atgggacttc taacctctcc agttacagct atccgtctgc tagctatcct cctcagactg 481 ctgtggggtc tgggtacagc cctggggggg caccgcctcc gccttcagcg tacctgcctt 541 caggaattcc tgctcccacc cccctacccc ccaccactgt tcctggctac acctaccagg 601 gccatggttt gacacctatt gcaccgtcgg ctctgacaaa cagttcagca agttctctca 661 aaaggaaagc tttctacatg gcagggcaag gagatatgga ctccagttat ggaaattaca 721 gctatggcca acagagatct acacagagtc ctatgtacag aatgcccgac aacagcattt 781 caaacacaaa tcgggggaat ggctttgaca gaagtgctga aacatcatcc ttagcattta 841 agccaacgaa gcagctaatg tcctctgaac agcaaaggaa attcagcagc cagtccagta 901 gggctctgac ccctccttcc tacagtactg ctaaaaattc attgggatca agatccagtg 961 aatcctttgg gaagtacaca tcgccagtaa tgagtgagca tggggacgag cacaggcagc 1021 tcctctctca cccaatgcaa ggccctggac tccgtgcagc tacctcatcc aaccactctg 1081 tggacgagca actgaagaat actgacacgc acctcatcga cctggtaacc aatgagatta 1141 tcacccaagg acctccagtg gactggaatg acattgctgg tctcgacctg gtgaaggctg 1201 tcattaaaga ggaggtttta tggccagtgt tgaggtcaga cgcgttcagt ggactgacgg 1261 ccttacctcg gagcatcctt ttatttggac ctcgggggac aggcaaaaca ttattgggca 1321 gatgcatcgc tagtcagctg ggggccacat ttttcaaaat tgccggttct ggactagtcg 1381 ccaagtggtt aggagaagca gagaaaatta tccatgcctc ttttcttgtg gccaggtgtc 1441 gccagccctc ggtgattttt gttagtgaca ttgacatgct tctctcctct caagtgaatg 1501 aggaacatag tccagtcagt cggatgagaa ccgaatttct gatgcaactg gacactgtac 1561 taacttcggc tgaggaccaa atcgtagtaa tttgtgccac cagtaaacca gaagaaatag 1621 atgaatccct tcggaggtac ttcatgaaac gacttttaat cccacttcct gacagcacag 1681 cgaggcacca gataatagta caactgctct cacagcacaa ttactgtctc aatgacaagg 1741 agtttgcact gctcgtccag cgcacagaag gcttttctgg actagatgtg gctcatttgt 1801 gtcaggaagc agtggtgggc cccctccatg ccatgccagc cacagacctt tcagccatta 1861 tgcccagcca gttgaggccc gttacatatc aagactttga aaatgctttc tgcaagattc 1921 agcctagcat atctcaaaag gAGCTTGATA TGTATGTTGA ATGGAACAAA ATGTTTGGTT 1981 GCAGTCAGTT GTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2041 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TTGAAAGTAT TTCGATTTCT 2101 TGGCTTTATA TATCTTGTGG AAAGGACGAC ATGCCTTCAT GGACACGATC TTCACGCGTT 2161 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att