Construct: ORF TRCN0000487852
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021386.1_s317c1
- DNA Barcode:
- TTCTTAGCTTGATTTAGTGCCCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SULF2 (55959)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487852
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55959 | SULF2 | sulfatase 2 | XM_011528916.2 | 99.6% | 99.8% | 226G>A;291T>C;2556_2557insTAAAAGCT |
| 2 | human | 55959 | SULF2 | sulfatase 2 | XM_011528915.2 | 99.4% | 99.7% | (many diffs) |
| 3 | human | 55959 | SULF2 | sulfatase 2 | NM_001161841.1 | 97.5% | 97.8% | (many diffs) |
| 4 | human | 55959 | SULF2 | sulfatase 2 | NM_018837.3 | 97.5% | 97.8% | (many diffs) |
| 5 | human | 55959 | SULF2 | sulfatase 2 | XM_005260458.3 | 97.5% | 97.8% | (many diffs) |
| 6 | human | 55959 | SULF2 | sulfatase 2 | XM_017027955.2 | 97.5% | 97.8% | (many diffs) |
| 7 | human | 55959 | SULF2 | sulfatase 2 | XM_006723830.2 | 97.5% | 97.8% | (many diffs) |
| 8 | human | 55959 | SULF2 | sulfatase 2 | XM_006723827.2 | 97.4% | 97.7% | (many diffs) |
| 9 | human | 55959 | SULF2 | sulfatase 2 | XM_006723828.2 | 97.4% | 97.7% | (many diffs) |
| 10 | human | 55959 | SULF2 | sulfatase 2 | XM_006723829.3 | 97.4% | 97.7% | (many diffs) |
| 11 | human | 55959 | SULF2 | sulfatase 2 | XM_011528913.1 | 97.4% | 97.7% | (many diffs) |
| 12 | human | 55959 | SULF2 | sulfatase 2 | XM_011528914.2 | 97.4% | 97.7% | (many diffs) |
| 13 | human | 55959 | SULF2 | sulfatase 2 | NM_198596.2 | 97.2% | 97.4% | (many diffs) |
| 14 | mouse | 72043 | Sulf2 | sulfatase 2 | NM_001252578.1 | 85% | 91.5% | (many diffs) |
| 15 | mouse | 72043 | Sulf2 | sulfatase 2 | NM_028072.5 | 85% | 91.5% | (many diffs) |
| 16 | mouse | 72043 | Sulf2 | sulfatase 2 | XM_006500209.3 | 85% | 91.5% | (many diffs) |
| 17 | mouse | 72043 | Sulf2 | sulfatase 2 | XM_006500211.2 | 85% | 91.5% | (many diffs) |
| 18 | mouse | 72043 | Sulf2 | sulfatase 2 | XM_017319268.1 | 85% | 91.5% | (many diffs) |
| 19 | mouse | 72043 | Sulf2 | sulfatase 2 | NM_001252579.1 | 81.9% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2622
- ORF length:
- 2556
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggg ccccccgagc ctcgtgctgt gcttgctgtc cgcaactgtg ttctccctgc 121 tgggtggaag ctcggccttc ctgtcgcacc accgcctgaa aggcaggttt cagagggacc 181 gcaggaacat ccgccccaac atcatcctgg tgctgacgga cgaccaggat gtggagctgg 241 gttccatgca ggtgatgaac aagacccggc gcatcatgga gcagggcggg acgcacttca 301 tcaacgcctt cgtgaccaca cccatgtgct gcccctcacg ctcctccatc ctcaccggca 361 agtacgtcca caaccacaac acctacacca acaatgagaa ctgctcctcg ccctcctggc 421 aggcacagca cgagagccgc acctttgccg tgtacctcaa tagcactggc taccggacag 481 ctttcttcgg gaagtatctt aatgaataca acggctccta cgtgccaccc ggctggaagg 541 agtgggtcgg actccttaaa aactcccgct tttataacta cacgctgtgt cggaacgggg 601 tgaaagagaa gcacggctcc gactactcca aggattacct cacagacctc atcaccaatg 661 acagcgtgag cttcttccgc acgtccaaga agatgtaccc gcacaggcca gtcctcatgg 721 tcatcagcca tgcagccccc cacggccctg aggattcagc cccacaatat tcacgcctct 781 tcccaaacgc atctcagcac atcacgccga gctacaacta cgcgcccaac ccggacaaac 841 actggatcat gcgctacacg gggcccatga agcccatcca catggaattc accaacatgc 901 tccagcggaa gcgcttgcag accctcatgt cggtggacga ctccatggag acgatttaca 961 acatgctggt tgagacgggc gagctggaca acacgtacat cgtatacacc gccgaccacg 1021 gttaccacat cggccagttt ggcctggtga aagggaaatc catgccatat gagtttgaca 1081 tcagggtccc gttctacgtg aggggcccca acgtggaagc cggctgtctg aatccccaca 1141 tcgtcctcaa cattgacctg gcccccacca tcctggacat tgcaggcctg gacatacctg 1201 cggatatgga cgggaaatcc atcctcaagc tgctggacac ggagcggccg gtgaatcggt 1261 ttcacttgaa aaagaagatg agggtctggc gggactcctt cttggtggag agaggcaagc 1321 tgctacacaa gagagacaat gacaaggtgg acgcccagga ggagaacttt ctgcccaagt 1381 accagcgtgt gaaggacctg tgtcagcgtg ctgagtacca gacggcgtgt gagcagctgg 1441 gacagaagtg gcagtgtgtg gaggacgcca cggggaagct gaagctgcat aagtgcaagg 1501 gccccatgcg gctgggcggc agcagagccc tctccaacct cgtgcccaag tactacgggc 1561 agggcagcga ggcctgcacc tgtgacagcg gggactacaa gctcagcctg gccggacgcc 1621 ggaaaaaact cttcaagaag aagtacaagg ccagctatgt ccgcagtcgc tccatccgct 1681 cagtggccat cgaggtggac ggcagggtgt accacgtagg cctgggtgat gccgcccagc 1741 cccgaaacct caccaagcgg cactggccag gggcccctga ggaccaagat gacaaggatg 1801 gtggggactt cagtggcact ggaggccttc ccgactactc agccgccaac cccattaaag 1861 tgacacatcg gtgctacatc ctagagaacg acacagtcca gtgtgacctg gacctgtaca 1921 agtccctgca ggcctggaaa gaccacaagc tgcacatcga ccacgagatt gaaaccctgc 1981 agaacaaaat taagaacctg agggaagtcc gaggtcacct gaagaaaaag cggccagaag 2041 aatgtgactg tcacaaaatc agctaccaca cccagcacaa aggccgcctc aagcacagag 2101 gctccagtct gcatcctttc aggaagggcc tgcaagagaa ggacaaggtg tggctgttgc 2161 gggagcagaa gcgcaagaag aaactccgca agctgctcaa gcgcctgcag aacaacgaca 2221 cgtgcagcat gccaggcctc acgtgcttca cccacgacaa ccagcactgg cagacggcgc 2281 ctttctggac actggggcct ttctgtgccT GCACCAGCGC CAACAATAAC ACGTACTGGT 2341 GCATGAGGAC CATCAATGAG ACTCACAATT TCCTCTTCTG TGAATTTGCA ACTGGCTTCC 2401 TAGAGTACTT TGATCTCAAC ACAGACCCCT ACCAGCTGAT GAATGCAGTG AACACACTGG 2461 ACAGGGATGT CCTCAACCAG CTACACGTAC AGCTCATGGA GCTGAGGAGC TGCAAGGGTT 2521 ACAAGCAGTG TAACCCCCGG ACTCGAAACA TGGACCTGGG ACTTAAAGAT GGAGGAAGCT 2581 ATGAGCAATA CAGGGGACAA CTGTGGGAAG GCTGGGAAGG TTAAAAGCTT GACCCAGCTT 2641 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2701 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2761 CTTGTGGAAA GGACGATTCT TAGCTTGATT TAGTGCCCTA ACGCGTTAAG TCgacaatca 2821 acctctggat tacaaaattt gtgaaagatt