Construct: ORF TRCN0000487893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020357.1_s317c1
- DNA Barcode:
- GTTCTAAGCATGATTGCCGCATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADCK1 (57143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_020421.4 | 100% | 100% | |
| 2 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366485.2 | 92.5% | 92.5% | 739_740ins117 |
| 3 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366487.2 | 90.1% | 90.1% | 856_1026del |
| 4 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366489.2 | 89.8% | 89.8% | 419_420ins159 |
| 5 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366486.2 | 87.3% | 87.3% | 1006_1007ins198 |
| 6 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001142545.2 | 86.9% | 86.9% | 217_218ins204 |
| 7 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366488.2 | 79.9% | 79.9% | 739_740ins117;889_890ins198 |
| 8 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366490.2 | 75.9% | 75.9% | 0_1ins378 |
| 9 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_017021476.1 | 75.9% | 75.9% | 0_1ins378 |
| 10 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_017021477.1 | 75.9% | 75.9% | 0_1ins378 |
| 11 | human | 57143 | ADCK1 | aarF domain containing kina... | XR_001750450.1 | 56% | 1_118del;974_1523del;2238_2801del | |
| 12 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_011536985.1 | 54.4% | 52.3% | (many diffs) |
| 13 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_011536987.2 | 50.5% | 46.3% | (many diffs) |
| 14 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_011536988.2 | 50.5% | 46.3% | (many diffs) |
| 15 | human | 57143 | ADCK1 | aarF domain containing kina... | NR_158988.2 | 46.8% | 1_73del;930_1009del;1723_3348del | |
| 16 | human | 57143 | ADCK1 | aarF domain containing kina... | NR_158989.2 | 42% | 1_73del;929_1387del;2102_3727del | |
| 17 | human | 57143 | ADCK1 | aarF domain containing kina... | NR_158987.2 | 36.7% | (many diffs) | |
| 18 | mouse | 72113 | Adck1 | aarF domain containing kina... | NM_001277296.1 | 85.4% | 88% | (many diffs) |
| 19 | mouse | 72113 | Adck1 | aarF domain containing kina... | NM_001277297.1 | 85.4% | 88% | (many diffs) |
| 20 | mouse | 72113 | Adck1 | aarF domain containing kina... | NM_028105.4 | 85.4% | 88% | (many diffs) |
| 21 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516245.3 | 77.9% | 80.1% | (many diffs) |
| 22 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516246.2 | 73.7% | 75.4% | (many diffs) |
| 23 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516251.2 | 63.5% | 65.5% | (many diffs) |
| 24 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516252.3 | 63.5% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1641
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaga aaggctctca agcttgcttc gtggaccagc atggctcttg 121 ctgcctctgg catctacttc tacagtaaca agtacttgga ccctaatgac tttggcgctg 181 tcagggtggg cagagcagtt gctacgacgg ctgtcatcag ttacgactac ctcacttccc 241 tgaagagtgt cccttatggc tcagaggagt acttgcagct gagatctaag gtgcaccttc 301 gctctgccag gcgtctctgt gagctctgct gtgccaaccg gggcaccttc atcaaggtgg 361 gccagcacct gggggctctg gactacctgt tgccagagga gtacaccagc acgctgaagg 421 tactgcacag ccaggctcca cagagcagca tgcaagagat ccgccaggtc atccgagaag 481 atctgggcaa ggagatccat gatttgttcc agagcttcga tgacacccct ctggggacgg 541 cctccctggc ccaggtccac aaggcagtgc tgcatgatgg gcggacggtg gccgtgaagg 601 tccagcaccc aaaggtgcgg gctcagagct cgaaggacat tctcctgatg gaggtgctcg 661 ttctggctgt gaagcagctg ttcccagagt ttgagtttat gtggcttgtg gatgaagcca 721 agaagaacct gcctttggag ctggatttcc tcaatgaagg gaggaatgct gagaaggtgt 781 cccagatgct caggcatttt gacttcttga aggtcccccg aatccactgg gacctgtcca 841 cggagcgggt cctcctgatg gagtttgtgg atggcgggca ggtcaatgac agagactaca 901 tggagaggaa caagatcgac gtcaatgaga tctcacgcca cctgggcaag atgtatagtg 961 agatgatctt cgtcaatggc ttcgtgcact gcgatcccca ccccggcaat gtactggtgc 1021 ggaagcaccc cggcacggga aaggcggaga ttgtcctgtt ggaccatggg ctttaccaga 1081 tgctcacgga agaattccgc ctgaattact gccacctctg gcagtctctg atctggactg 1141 acatgaagag agtgaaggag tacagccagc gactgggagc cggggatctc taccccttgt 1201 ttgcctgcat gctgacggcg cgatcgtggg actcggtcaa cagaggcatc agccaagctc 1261 ccgtcactgc cactgaggac ttagagattc gcaacaacgc ggccaactac ctcccccaga 1321 tcagccatct ccTCAACCAC GTGCCGCGCC AGATGCTGCT CATCTTGAAG ACCAACGACC 1381 TGCTGCGTGG CATTGAGGCC GCCCTGGGCA CCCGCGCCAG CGCCAGCTCC TTTCTCAACA 1441 TGTCACGTTG CTGCATCAGA GCGCTAGCTG AGCACAAGAA GAAGAATACC TGTTCATTCT 1501 TCAGAAGGAC CCAGATCTCT TTCAGCGAGG CCTTCAACTT ATGGCAGATC AACCTCCATG 1561 AGCTCATCCT GCGTGTGAAG GGGTTGAAGC TGGCTGACCG GGTCTTGGCC CTAATATGCT 1621 GGCTGTTCCC TGCTCCACTC TAGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TTCTAAGCAT 1801 GATTGCCGCA TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att