Transcript: Mouse XM_006516245.3

PREDICTED: Mus musculus aarF domain containing kinase 1 (Adck1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Adck1 (72113)
Length:
2604
CDS:
624..2078

Additional Resources:

NCBI RefSeq record:
XM_006516245.3
NBCI Gene record:
Adck1 (72113)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006516245.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023848 GAATTTGTAGAGGGAGGTCAA pLKO.1 1290 CDS 100% 4.050 5.670 N Adck1 n/a
2 TRCN0000023845 GACTCAGAGATTCGCAATAAT pLKO.1 1707 CDS 100% 15.000 12.000 N Adck1 n/a
3 TRCN0000361858 ATATGGCTCTGAGGAGTATTT pLKO_005 683 CDS 100% 13.200 9.240 N Adck1 n/a
4 TRCN0000368227 GGACTCAGAGATTCGCAATAA pLKO_005 1706 CDS 100% 13.200 9.240 N Adck1 n/a
5 TRCN0000361859 GGAGAGTGAGATGGGATAAAG pLKO_005 2267 3UTR 100% 13.200 9.240 N Adck1 n/a
6 TRCN0000368747 GGGCAAGGAGATCCACGATTT pLKO_005 914 CDS 100% 10.800 7.560 N Adck1 n/a
7 TRCN0000023847 CTGTGGCAGATCAACCTTCAT pLKO.1 1968 CDS 100% 4.950 3.465 N Adck1 n/a
8 TRCN0000197154 GTCTCTGATCTGGACTGACAT pLKO.1 1553 CDS 100% 4.950 3.465 N ADCK1 n/a
9 TRCN0000352787 GTCTCTGATCTGGACTGACAT pLKO_005 1553 CDS 100% 4.950 3.465 N ADCK1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006516245.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03795 pDONR223 100% 77.9% 80.1% None (many diffs) n/a
2 ccsbBroad304_03795 pLX_304 0% 77.9% 80.1% V5 (many diffs) n/a
3 TRCN0000472002 CCAATTCCCTATTCTTCGGTAGGT pLX_317 28% 77.9% 80.1% V5 (many diffs) n/a
4 ccsbBroadEn_15119 pDONR223 0% 77.9% 80.1% None (many diffs) n/a
5 ccsbBroad304_15119 pLX_304 0% 77.9% 80.1% V5 (many diffs) n/a
6 TRCN0000467145 AATAATGTTTAACCGTTACCCGGA pLX_317 21.3% 77.9% 80.1% V5 (many diffs) n/a
7 TRCN0000487893 GTTCTAAGCATGATTGCCGCATTA pLX_317 20.1% 77.9% 80.1% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV