Construct: ORF TRCN0000487938

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020081.1_s317c1
DNA Barcode:
CACTCCCCATTACGCCACTCGACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCNC4 (3749)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487938
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3749 KCNC4 potassium voltage-gated cha... NM_004978.5 99.9% 99.8% 1905_1906insG
2 human 3749 KCNC4 potassium voltage-gated cha... NM_001039574.3 97.2% 94.8% (many diffs)
3 human 3749 KCNC4 potassium voltage-gated cha... XM_006710625.4 96.8% 95.9% (many diffs)
4 human 3749 KCNC4 potassium voltage-gated cha... XM_006710627.4 96% 96.3% (many diffs)
5 human 3749 KCNC4 potassium voltage-gated cha... XM_011541401.3 95.6% 95.4% 1823_1825delAGAinsCAT;1827_1828ins79
6 human 3749 KCNC4 potassium voltage-gated cha... XM_024446790.1 93.9% 92.7% (many diffs)
7 human 3749 KCNC4 potassium voltage-gated cha... XM_011541404.2 48.5% 34.8% (many diffs)
8 human 3749 KCNC4 potassium voltage-gated cha... XM_011541402.2 45.1% 34.3% 677_678ins937;969_1209delinsG
9 human 3749 KCNC4 potassium voltage-gated cha... XM_011541403.2 45.1% 34.3% 677_678ins937;969_1209delinsG
10 human 3749 KCNC4 potassium voltage-gated cha... NR_036437.2 42.1% 1_1186del;3092_4516delinsG
11 mouse 99738 Kcnc4 potassium voltage gated cha... NM_001356447.1 86.4% 90.9% (many diffs)
12 mouse 99738 Kcnc4 potassium voltage gated cha... NM_145922.3 85.3% 92.9% (many diffs)
13 mouse 99738 Kcnc4 potassium voltage gated cha... XM_006502475.4 83.1% 89.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1977
ORF length:
1908
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gatcagctcg gtgtgtgtct cctcctaccg cgggcgcaag tcggggaaca
121agcctccgtc caaaacatgt ctgaaggagg agatggccaa gggcgaggcg tcggagaaga
181tcatcatcaa cgtgggcggc acgcgacatg agacctaccg cagcaccctg cgcaccctac
241cgggaacccg cctcgcctgg ctggccgacc ccgacggcgg gggccggccc gagaccgatg
301gcggcggtgt gggtagcagc ggcagcagcg gcggcggggg ctgcgagttc ttcttcgaca
361ggcacccggg cgtcttcgcc tacgtgctca actactaccg caccggcaag ctgcactgcc
421ccgcggacgt gtgcgggccg ctcttcgaag aggagctcac cttctggggc atcgacgaga
481ccgacgtgga accctgctgc tggatgacct accggcagca ccgcgacgcc gaggaggcgc
541tcgacatctt cgagagcccg gacggaggcg gcagcggcgc ggggcccagc gacgaggccg
601gcgacgatga gcgggagctg gccctgcagc gactgggccc ccacgaggga ggcgcgggcc
661atggcgccgg gtctgggggc tgccgcggct ggcagccccg catgtgggcg ctcttcgagg
721atccctactc ctcccgggcc gctagggtag tggcctttgc ctctctcttc ttcatcctgg
781tctccatcac cactttctgc ctggagaccc atgaggcctt taatatcgac cgcaacgtga
841cagagatcct ccgcgtaggg aacatcacca gcgtgcactt ccggcgggag gtagagacag
901agcccatcct gacctacatc gagggcgtat gtgtgctgtg gttcacactg gagttcctgg
961tgcgcatcgt gtgctgcccc gacacgctgg acttcgtcaa gaacctgctc aacatcatcg
1021actttgtggc catcctgccc ttctacctgg aggtggggct gagcggcctg tcatccaagg
1081cggcccgcga cgtgctgggc ttcctgcgcg tggtgcgctt cgtgcgcatc ctgcgtatct
1141tcaagctcac acgccacttc gtggggctac gcgtgctggg ccacaccctg agggccagca
1201ccaatgagtt cctgctgctt atcatcttcc tggccctggg tgtgctcatc tttgccacca
1261tgatctacta cgctgagcgc attggggcca ggccctccga ccctcggggt aatgaccaca
1321ccgacttcaa gaacatcccc attggcttct ggtgggctgt ggtcaccatg acgacactgg
1381gctacggaga catgtacccc aagacgtggt caggcatgct ggtaggggca ctgtgtgcac
1441tggctggcgt gctcaccatc gccatgccgg tgcctgtcat cgtcaacaac ttcggcatgt
1501actactccct ggccatggcc aagcagaagc tgcccaagaa acggaagaag cacgtgccac
1561ggccggcgca gctggagtca cccatgtact gcaagtctga ggagacttcc ccccgggaca
1621gcacctgcag tgataccagc ccccctgccc gggaagaggG TATGATCGAG AGGAAACGGG
1681CAGACTCTAA GCAGAATGGC GATGCCAACG CAGTGCTGTC TGATGAGGAG GGAGCTGGCC
1741TCACCCAACC CCTGGCCTCC TCCCCGACCC CCGAGGAGCG CCGGGCCCTG CGACGCTCCA
1801CCACTCGAGA CAGAAACAAG AAGGCAGCTG CCTGCTTCCT GCTCAGCACT GGGGACTATG
1861CCTGCGCCGA TGGTAGTGTC CGGAAAGGCA CATTCGTCCT CCGTGACCTT CCCCTTCAGC
1921ATTCACCTGA GGCTGCATGC CCTCCAACTG CTGGGACTCT GTTCCTGCCA CATGACCCAG
1981CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
2041ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
2101TATCTTGTGG AAAGGACGAC ACTCCCCATT ACGCCACTCG ACGACGCGTT AAGTCgacaa
2161tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)