Construct: ORF TRCN0000488025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019911.1_s317c1
- DNA Barcode:
- TTGTTCGGACCCTTCATACATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOXL2 (4017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4017 | LOXL2 | lysyl oxidase like 2 | NM_002318.3 | 99.8% | 99.7% | (many diffs) |
| 2 | mouse | 94352 | Loxl2 | lysyl oxidase-like 2 | NM_033325.2 | 84% | 86.1% | (many diffs) |
| 3 | mouse | 94352 | Loxl2 | lysyl oxidase-like 2 | XM_006519747.2 | 84% | 86.1% | (many diffs) |
| 4 | mouse | 94352 | Loxl2 | lysyl oxidase-like 2 | XM_006519744.2 | 83.3% | 85.3% | (many diffs) |
| 5 | mouse | 94352 | Loxl2 | lysyl oxidase-like 2 | XM_006519745.2 | 83.3% | 85.3% | (many diffs) |
| 6 | mouse | 94352 | Loxl2 | lysyl oxidase-like 2 | XM_006519746.2 | 83.3% | 85.3% | (many diffs) |
| 7 | mouse | 100862072 | Gm21451 | predicted gene, 21451 | XM_006519897.3 | 77.1% | 78.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2394
- ORF length:
- 2325
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagaggcct ctgtgctccc acctctgcag ctgcctggct atgctggccc 121 tcctgtcccc cctgagcctg gcacagtatg acagctggcc ccattacccc gagtacttcc 181 agcaaccggc tcctgagtat caccagcccc aggcccccgc caacgtggcc aagattcagc 241 tgcgcctggc tgggcagaag aggaagcaca gcgagggccg ggtggaggtg tactatgatg 301 gccagtgggg caccgtgtgc gatgacgact tctccatcca cgctgcccac gtcgtctgcc 361 gggagctggg ctacgtggag gccaagtcct ggactgccag ctcctcctac ggcaagggag 421 aagggcccat ctggttagac aatctccact gtactggcaa cgaggcgacc cttgcagcat 481 gcacctccaa tggctggggc gtcactgact gcaagcacac ggaggatgtc ggtgtggtgt 541 gcagcgacaa aaggattcct gggttcaaat ttgacaattc gttgatcaac cagatagaga 601 acctgaatat ccaggtggag gacattcgga ttcgagccat cctctcaacc taccgcaagc 661 gcaccccagt gatggagggc tacgtggagg tgaaggaggg caagacctgg aagcagatct 721 gtgacaagca ctggacggcc aagaattccc gcgtggtctg cggcatgttt ggcttccctg 781 gggagaggac atacaatacc aaagtgtaca aaatgtttgc ctcacggagg aagcagcgct 841 actggccatt ctccatggac tgcaccggca cagaggccca catctccagc tgcaagctgg 901 gcccccaggt gtcactggac cccatgaaga atgtcacctg cgagaatggg ctaccggccg 961 tggtgagttg tgtgcctggg caggtcttca gccctgacgg accctcgaga ttccggaaag 1021 cgtacaagcc agagcaaccc ctggtgcgac tgagaggcgg tgcctacatc ggggagggcc 1081 gcgtggaggt gctcaaaaat ggagagtggg ggaccgtctg cgacgacaag tgggacctgg 1141 tgtcggccag tgtggtctgc agagagctgg gctttgggag tgccaaagag gcagtcactg 1201 gctcccgact ggggcaaggg atcggaccca tccacctcaa cgagatccag tgcacaggca 1261 atgagaagtc cattatagac tgcaagttca atgccgagtc tcagggctgc aaccacgagg 1321 aggatgctgg tgtgagatgc aacacccctg ccatgggctt gcagaagaag ctgcgcctga 1381 acggcggccg caatccctac gagggccgag tggaggtgct ggtggagaga aacgggtccc 1441 ttgtgtgggg gatggtgtgt ggccaaaact ggggcatcgt ggaggccatg gtggtctgcc 1501 gccagctggg cctgggattc gccagcaacg ccttccagga gacctggtat tggcacggag 1561 atgtcaacag caacaaagtg gtcatgagtg gagtgaagtg ctcgggaacg gagctgtccc 1621 tggcgcactg ccgccacgac ggggaggacg tggcctgccc ccagggcgga gtgcagtacg 1681 gggccggagt tgcctgctca gaaaccgccc ctgacctggt cctcaatgcg gagatggtgc 1741 agcagaccac ctacctggag gaccggccca tgttcctgct gcagtgtgcc atggaggaga 1801 actgcctctc ggcctcagcc gcgcagaccg accccaccac gggctaccgc cggctcctgc 1861 gcttctcctc ccagatccac aacaatggcc agtccgactt ccggcccaag aacggccgcc 1921 acgcgtggat ctggcacgac tgtcacaggc actaccacag catggaggtg ttcacccact 1981 atgacctgct gaacctcaat ggcaccaagg tggcagaggg ccacaaggcc agcttctgct 2041 tggaggacac agaatgtgaa ggagacatcc agaagaatta cgagtgtgcc aacTTCGGCG 2101 ATCAGGGCAT CACCATGGGC TGCTGGGACA TGTACCGCCA TGACATCGAC TGCCAGTGGG 2161 TTGACATCAC TGACGTGCCC CCTGGAGACT ACCTGTTCCA GGTTGTTATT AACCCCAACT 2221 TCGAGGTTGC AGAATCCGAT TACTCCAACA ACATCATGAA ATGCAGGAGC CGCTATGACG 2281 GCCACCGCAT CTGGATGTAC AACTGCCACA TAGGTGGTTC CTTCAGCGAA GAGACGGAAA 2341 AAAAGTTTGA GCACTTCAGC GGGCTCTTAA ACAACCAGCT GTCCCCGCAG GACCCAGCTT 2401 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2461 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2521 CTTGTGGAAA GGACGATTGT TCGGACCCTT CATACATCTA ACGCGTTAAG TCgacaatca 2581 acctctggat tacaaaattt gtgaaagatt