Construct: ORF TRCN0000488054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020436.1_s317c1
- DNA Barcode:
- AAGGTTTGCCGATAAATCTTAGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K2 (5605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5605 | MAP2K2 | mitogen-activated protein k... | NM_030662.3 | 100% | 100% | |
| 2 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_006722799.2 | 76.7% | 76.7% | 705_706ins279 |
| 3 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026989.1 | 75.3% | 67.6% | (many diffs) |
| 4 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026990.1 | 70.8% | 50% | (many diffs) |
| 5 | human | 407835 | LOC407835 | mitogen-activated protein k... | NR_002144.1 | 65.9% | (many diffs) | |
| 6 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026991.1 | 61.6% | 60.2% | (many diffs) |
| 7 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_001347144.1 | 87.2% | 94.2% | (many diffs) |
| 8 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_023138.5 | 87% | 94% | (many diffs) |
| 9 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513684.3 | 45.2% | 48.2% | (many diffs) |
| 10 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513683.3 | 45.1% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1272
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctggcc cggaggaagc cggtgctgcc ggcgctcacc atcaacccta 121 ccatcgccga gggcccatcc cctaccagcg agggcgcctc cgaggcaaac ctggtggacc 181 tgcagaagaa gctggaggag ctggaacttg acgagcagca gaagaagcgg ctggaagcct 241 ttctcaccca gaaagccaag gtcggcgaac tcaaagacga tgacttcgaa aggatctcag 301 agctgggcgc gggcaacggc ggggtggtca ccaaagtcca gcacagaccc tcgggcctca 361 tcatggccag gaagctgatc caccttgaga tcaagccggc catccggaac cagatcatcc 421 gcgagctgca ggtcctgcac gaatgcaact cgccgtacat cgtgggcttc tacggggcct 481 tctacagtga cggggagatc agcatttgca tggaacacat ggacggcggc tccctggacc 541 aggtgctgaa agaggccaag aggattcccg aggagatcct ggggaaagtc agcatcgcgg 601 ttctccgggg cttggcgtac ctccgagaga agcaccagat catgcaccga gatgtgaagc 661 cctccaacat cctcgtgaac tctagagggg agatcaagct gtgtgacttc ggggtgagcg 721 gccagctcat cgactccatg gccaactcct tcgtgggcac gcgctcctac atggctccgg 781 agcggttgca gggcacacat tactcggtgc agtcggacat ctggagcatg ggcctgtccc 841 tggtggagct ggccgtcgga aggtacccca tccccccgcc cgacgccaaa gagctggagg 901 ccatctttgg ccggcccgtg gtcgacgggg aagaaggaga gcctcacagc atctcgcctc 961 ggccgaggcc ccccgggcgc cccgtcagcg gtcacgggat GGATAGCCGG CCTGCCATGG 1021 CCATCTTTGA ACTCCTGGAC TATATTGTGA ACGAGCCACC TCCTAAGCTG CCCAACGGTG 1081 TGTTCACCCC CGACTTCCAG GAGTTTGTCA ATAAATGCCT CATCAAGAAC CCAGCGGAGC 1141 GGGCGGACCT GAAGATGCTC ACAAACCACA CCTTCATCAA GCGGTCCGAG GTGGAAGAAG 1201 TGGATTTTGC CGGCTGGTTG TGTAAAACCC TGCGGCTGAA CCAGCCCGGC ACACCCACGC 1261 GCACCGCCGT GTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAGGTTTGCC GATAAATCTT 1441 AGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt