Construct: ORF TRCN0000488120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022020.1_s317c1
- DNA Barcode:
- GCAAGGCAGAAGTCCCTCTATTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRNA7 (1139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_000746.6 | 99.9% | 100% | 690G>A |
| 2 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_001190455.3 | 94.4% | 94.5% | 55_141del;777G>A |
| 3 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521176.3 | 91.2% | 87.1% | (many diffs) |
| 4 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021883.2 | 81.5% | 72.1% | (many diffs) |
| 5 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021882.1 | 81.4% | 72.1% | (many diffs) |
| 6 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_139320.1 | 80% | 78% | (many diffs) |
| 7 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522153.2 | 80% | 78% | (many diffs) |
| 8 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521177.2 | 79.3% | 77.4% | (many diffs) |
| 9 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521178.3 | 75.6% | 63.3% | 690G>A;879_880ins110;1140_1141ins256 |
| 10 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021884.1 | 63.8% | 63.9% | 0_1ins543;147G>A |
| 11 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_148911.1 | 63.6% | 63.9% | (many diffs) |
| 12 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_005254750.3 | 63.6% | 63.9% | (many diffs) |
| 13 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522155.3 | 55.7% | 41.7% | (many diffs) |
| 14 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NR_046324.1 | 39.1% | (many diffs) | |
| 15 | mouse | 11441 | Chrna7 | cholinergic receptor, nicot... | NM_007390.3 | 87.9% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1575
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcgctgctcg ccgggaggcg tctggctggc gctggccgcg tcgctcctgc 121 acgtgtccct gcaaggcgag ttccagagga agctttacaa ggagctggtc aagaactaca 181 atcccttgga gaggcccgtg gccaatgact cgcaaccact caccgtctac ttctccctga 241 gcctcctgca gatcatggac gtggatgaga agaaccaagt tttaaccacc aacatttggc 301 tgcaaatgtc ttggacagat cactatttac agtggaatgt gtcagaatat ccaggggtga 361 agactgttcg tttcccagat ggccagattt ggaaaccaga cattcttctc tataacagtg 421 ctgatgagcg ctttgacgcc acattccaca ctaacgtgtt ggtgaattct tctgggcatt 481 gccagtacct gcctccaggc atattcaaga gttcctgcta catcgatgta cgctggtttc 541 cctttgatgt gcagcactgc aaactgaagt ttgggtcctg gtcttacgga ggctggtcct 601 tggatctgca gatgcaggag gcagatatca gtggctatat ccccaatgga gaatgggacc 661 tagtgggaat ccccggcaag aggagtgaaa ggttctatga gtgctgcaaa gagccctacc 721 ccgatgtcac cttcacagtg accatgcgcc gcaggacact ctactatggc ctcaacctgc 781 tgatcccctg tgtgctcatc tccgccctcg ccctgctggt gttcctgctt cctgcagatt 841 ccggggagaa gatttccctg gggataacag tcttactctc tcttaccgtc ttcatgctgc 901 tcgtggctga gatcatgccc gcaacatccg attcggtacc attgatagcc cagtacttcg 961 ccagcaccat gatcatcgtg ggcctctcgg tggtggtgac agtgatcgtg ctgcagtacc 1021 accaccacga ccccgacggg ggcaagatgc ccaagtggac cagagtcatc cttctgaact 1081 ggtgcgcgtg gttcctgcga atgaagaggc ccggggagga caaggtgcgc ccggcctgcc 1141 agcacaagca gcggcgctgc agcctggcca gtgtggagat gagcgccgtg gcgccgccgc 1201 ccgccagcaa cgggaacctg ctgtacatcg gcttccgcgg cctggacggc gtgcactgtg 1261 tcccgacccc cgactctggg gtagtgtgtg gccgcatggc ctgctccccc acgcacgatg 1321 agcaccTCCT GCACGGCGGG CAACCCCCCG AGGGGGACCC GGACTTGGCC AAGATCCTGG 1381 AGGAGGTCCG CTACATTGCC AACCGCTTCC GCTGCCAGGA CGAAAGCGAG GCGGTCTGCA 1441 GCGAGTGGAA GTTCGCCGCC TGTGTGGTGG ACCGCCTGTG CCTCATGGCC TTCTCGGTCT 1501 TCACCATCAT CTGCACCATC GGCATCCTGA TGTCGGCTCC CAACTTCGTG GAGGCCGTGT 1561 CCAAAGACTT TGCGTAAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCAAGGC AGAAGTCCCT 1741 CTATTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt