Construct: ORF TRCN0000488183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019500.1_s317c1
- DNA Barcode:
- AGTGTCCGTCGATCTTTACTTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MINK1 (50488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_001024937.4 | 99.9% | 100% | 3429C>A |
| 2 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_153827.5 | 98.4% | 98.4% | 1739_1798del;3489C>A |
| 3 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_011523906.1 | 98.2% | 98.2% | 939_940insGGTGAGAAA;1730_1789del;3480C>A |
| 4 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024705.1 | 97.8% | 97.8% | 1347_1433del;3516C>A |
| 5 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721531.2 | 96.3% | 96.3% | 1347_1433del;1826_1885del;3576C>A |
| 6 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721532.2 | 96.3% | 96.3% | (many diffs) |
| 7 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024704.1 | 96.1% | 96.1% | (many diffs) |
| 8 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_015716.5 | 95.6% | 95.7% | 1739_1798del;2085_2086ins111;3378C>A |
| 9 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_170663.5 | 95.1% | 95.1% | (many diffs) |
| 10 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_006721536.2 | 93.6% | 93.6% | (many diffs) |
| 11 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024706.1 | 93.5% | 93.6% | (many diffs) |
| 12 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024707.2 | 92.3% | 92.3% | (many diffs) |
| 13 | human | 50488 | MINK1 | misshapen like kinase 1 | NM_001321236.2 | 91.5% | 91.5% | (many diffs) |
| 14 | human | 50488 | MINK1 | misshapen like kinase 1 | XM_017024708.1 | 91.1% | 91.1% | (many diffs) |
| 15 | human | 50488 | MINK1 | misshapen like kinase 1 | XR_001752522.2 | 76.8% | (many diffs) | |
| 16 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_001045959.1 | 89.2% | 95.6% | (many diffs) |
| 17 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533681.3 | 88.7% | 95% | (many diffs) |
| 18 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_001045964.1 | 88.6% | 95% | (many diffs) |
| 19 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533679.2 | 87.3% | 93.6% | (many diffs) |
| 20 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533677.2 | 86.8% | 93% | (many diffs) |
| 21 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533678.2 | 86.8% | 93% | (many diffs) |
| 22 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_016713.2 | 86.6% | 92.8% | (many diffs) |
| 23 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | NM_176893.2 | 86.1% | 92.3% | (many diffs) |
| 24 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_017314646.1 | 84.8% | 90.9% | (many diffs) |
| 25 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_006533682.3 | 84.3% | 90.3% | (many diffs) |
| 26 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XM_017314645.1 | 84.2% | 90.3% | (many diffs) |
| 27 | mouse | 50932 | Mink1 | misshapen-like kinase 1 (ze... | XR_388490.3 | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 4029
- ORF length:
- 3936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tccacggagg ccatgggcga cccagccccc gcccgcagcc 121 tggacgacat cgacctgtcc gccctgcggg accctgctgg gatctttgag cttgtggagg 181 tggtcggcaa tggaacctac ggacaggtgt acaagggtcg gcatgtcaag acggggcagc 241 tggctgccat caaggtcatg gatgtcacgg aggacgagga ggaagagatc aaacaggaga 301 tcaacatgct gaaaaagtac tctcaccacc gcaacatcgc cacctactac ggagccttca 361 tcaagaagag ccccccggga aacgatgacc agctctggct ggtgatggag ttctgtggtg 421 ctggttcagt gactgacctg gtaaagaaca caaaaggcaa cgccctgaag gaggactgta 481 tcgcctatat ctgcagggag atcctcaggg gtctggccca tctccatgcc cacaaggtga 541 tccatcgaga catcaagggg cagaatgtgc tgctgacaga gaatgctgag gtcaagctag 601 tggattttgg ggtgagtgct cagctggacc gcaccgtggg cagacggaac actttcattg 661 ggactcccta ctggatggct ccagaggtca tcgcctgtga tgagaaccct gatgccacct 721 atgattacag gagtgatatt tggtctctag gaatcacagc catcgagatg gcagagggag 781 ccccccctct gtgtgacatg caccccatgc gagccctctt cctcattcct cggaaccctc 841 cgcccaggct caagtccaag aagtggtcta agaagttcat tgacttcatt gacacatgtc 901 tcatcaagac ttacctgagc cgcccaccca cggagcagct actgaagttt cccttcatcc 961 gggaccagcc cacggagcgg caggtccgca tccagcttaa ggaccacatt gaccgatccc 1021 ggaagaagcg gggtgagaaa gaggagacag aatatgagta cagcggcagc gaggaggaag 1081 atgacagcca tggagaggaa ggagagccaa gctccatcat gaacgtgcct ggagagtcga 1141 ctctacgccg ggagtttctc cggctccagc aggaaaataa gagcaactca gaggctttaa 1201 aacagcagca gcagctgcag cagcagcagc agcgagaccc cgaggcacac atcaaacacc 1261 tgctgcacca gcggcagcgg cgcatagagg agcagaagga ggagcggcgc cgcgtggagg 1321 agcaacagcg gcgggagcgg gagcagcgga agctgcagga gaaggagcag cagcggcggc 1381 tggaggacat gcaggctctg cggcgggagg aggagcggcg gcaggcggag cgcgagcagg 1441 aatacaagcg gaagcagctg gaggagcagc ggcagtcaga acgtctccag aggcagctgc 1501 agcaggagca tgcctacctc aagtccctgc agcagcagca acagcagcag cagcttcaga 1561 aacagcagca gcagcagctc ctgcctgggg acaggaagcc cctgtaccat tatggtcggg 1621 gcatgaatcc cgctgacaaa ccagcctggg cccgagaggt agaagagaga acaaggatga 1681 acaagcagca gaactctccc ttggccaaga gcaagccagg cagcacgggg cctgagcccc 1741 ccatccccca ggcctcccca gggcccccag gacccctttc ccagactcct cctatgcaga 1801 ggccggtgga gccccaggag ggaccgcaca agtccctgca ggaccagccc acccgaaacc 1861 tggctgcctt cccagcctcc catgaccccg accctgccat ccccgcaccc actgccacgc 1921 ccagtgcccg aggagctgtc atccgccaga attcagaccc cacctctgaa ggacctggcc 1981 ccagcccgaa tcccccagcc tgggtccgcc cagataacga ggccccaccc aaggtgcctc 2041 agaggacctc atctatcgcc actgccctta acaccagtgg ggccggaggg tcccggccag 2101 cccaggcagt ccgtgccaga cctcgcagca actccgcctg gcaaatctat ctgcaaaggc 2161 gggcagagcg gggcacccca aagcctccag ggccccctgc tcagccccct ggcccgccca 2221 acgcctctag taaccccgac ctcaggagga gcgaccctgg ctgggaacgc tcggacagcg 2281 tccttccagc ctctcacggg cacctccccc aggctggctc actggagcgg aaccgcgtgg 2341 gagtctcctc caaaccggac agctcccctg tgctctcccc tgggaataaa gccaagcccg 2401 acgaccaccg ctcacggcca ggccggcccg cagactttgt gttgctgaaa gagcggactc 2461 tggacgaggc ccctcggcct cccaagaagg ccatggacta ctcgtcgtcc agcgaggagg 2521 tggaaagcag tgaggacgac gaggaggaag gcgaaggcgg gccagcagag gggagcagag 2581 atacccctgg gggccgcagc gatggggata cagacagcgt cagcaccatg gtggtccacg 2641 acgtcgagga gatcaccggg acccagcccc catacggggg cggcaccatg gtggtccagc 2701 gcacccctga agaggagcgg aacctgctgc atgctgacag caatgggtac acaaacctgc 2761 ctgacgtggt ccagcccagc cactcaccca ccgagaacag caaaggccaa agcccaccct 2821 cgaaggatgg gagtggtgac taccagtctc gtgggctggt aaaggcccct ggcaagagct 2881 cgttcacgat gtttgtggat ctagggatct accagcctgg aggcagtggg gacagcatcc 2941 ccatcacagc cctagtgggt ggagagggca ctcggctcga ccagctgcag tacgacgtga 3001 ggaagggttc tgtggtcaac gtgaatccca ccaacacccg ggcccacagt gagacccctg 3061 agatccggaa gtacaagaag cgattcaact ccgagatcct ctgtgcagcc ctttgggggg 3121 tcaacctgct ggtgggcacg gagaacgggc tgatgttgct ggaccgaagt gggcagggca 3181 aggtgtatgg actcattggg cggcgacgct tccagcagat ggatgtgctg gaggggctca 3241 acctgctcat caccatctca gggaaaagga acaaactgcg ggtgtattac ctgtcctggc 3301 tccggaacaa gattctgcac aatgacccag aagtggagaa gaagcagggc tggaccaccg 3361 tgggggacat ggagggctgc gggcactacc gtgttgtgaa atacgagcgg attaagttcc 3421 tggtcatcgc cctcaagagc tccgtggagg tgtatgcctg ggcccccaaa ccctaccaca 3481 aattcatggc cttcaagtcc tttgccgacc tcccccaccg acctctgctg gtcgacctga 3541 cagtagagga ggggcagcgg ctcaaggtca tctatggctc cagtgctggc ttccatgctg 3601 tggatgtcga ctcggggaac agctatgaca tctacatccc tgtgcacatc cagagccaga 3661 tcacgcccca tgccatcatc ttcctcccca acaccGACGG CATGGAGATG CTGCTGTGCT 3721 ACGAGGACGA GGGTGTCTAC GTCAACACGT ACGGGCGCAT CATTAAGGAT GTGGTGCTGC 3781 AGTGGGGGGA GATGCCTACT TCTGTGGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG 3841 GTGAGAAAGC CATTGAGATC CGCTCTGTGG AGACGGGCCA CCTCGACGGG GTCTTCATGC 3901 ACAAACGAGC TCAGAGGCTC AAGTTCCTGT GTGAGCGGAA TGACAAGGTG TTTTTTGCCT 3961 CAGTCCGCTC TGGGGGCAGC AGCCAAGTTT ACTTCATGAC TCTGAACCGT AACTGCATCA 4021 TGAACTGGAA GGGTGGGCGC GCCGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 4081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 4141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GTGTCCGTCG 4201 ATCTTTACTT GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 4261 att