Construct: ORF TRCN0000488199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020618.1_s317c1
- DNA Barcode:
- AACGTGGACCCGTCCGTGGCAGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- JAK2 (3717)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322194.1 | 99.8% | 100% | (many diffs) |
| 2 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322195.1 | 99.8% | 100% | (many diffs) |
| 3 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322196.1 | 99.8% | 100% | (many diffs) |
| 4 | human | 3717 | JAK2 | Janus kinase 2 | NM_004972.3 | 99.8% | 100% | (many diffs) |
| 5 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322204.1 | 86.6% | 86.8% | (many diffs) |
| 6 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322198.1 | 64.1% | 64.2% | (many diffs) |
| 7 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322199.1 | 64.1% | 64.2% | (many diffs) |
| 8 | mouse | 16452 | Jak2 | Janus kinase 2 | NM_001048177.2 | 88.5% | 93.7% | (many diffs) |
| 9 | mouse | 16452 | Jak2 | Janus kinase 2 | NM_008413.3 | 88.5% | 93.7% | (many diffs) |
| 10 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_011247155.2 | 88.5% | 93.7% | (many diffs) |
| 11 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526713.3 | 85% | 89.8% | (many diffs) |
| 12 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526714.3 | 73.4% | 78% | (many diffs) |
| 13 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_017318068.1 | 57.1% | 61.2% | (many diffs) |
| 14 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526715.3 | 46.4% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3468
- ORF length:
- 3396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaatg gcctgcctta cgatgacaga aatggaggga acatccacct 121 cttctatata tcagaatggt gatatttctg gaaatgccaa ttctatgaag caaatagatc 181 cagttcttca ggtgtatctt taccattccc ttgggaaatc tgaggcagat tatctgacct 241 ttccatctgg ggagtatgtt gcagaagaaa tctgtattgc tgcttctaaa gcttgtggta 301 tcacacctgt gtatcataat atgtttgctt taatgagtga aacagaaagg atctggtatc 361 cacccaacca tgtcttccat atagatgagt caaccaggca taatgtactc tacagaataa 421 gattttactt tcctcgttgg tattgcagtg gcagcaacag agcctatcgg catggaatat 481 ctcgaggtgc tgaagctcct cttcttgatg actttgtcat gtcttacctc tttgctcagt 541 ggcggcatga ttttgtgcat ggatggataa aagtacctgt gactcatgaa acacaggaag 601 aatgtcttgg gatggcagtg ttagatatga tgagaatagc caaagaaaac gatcaaaccc 661 cactggccat ctataactct atcagctaca agacattctt accaaaatgt attcgagcaa 721 agatccaaga ctatcatatt ttgacaagga agcgaataag gtacagattt cgcagattta 781 ttcagcaatt cagccaatgc aaagccactg ccagaaactt gaaacttaag tatcttataa 841 atctggaaac tctgcagtct gccttctaca cagagaaatt tgaagtaaaa gaacctggaa 901 gtggtccttc aggtgaggag atttttgcaa ccattataat aactggaaat ggtggaattc 961 agtggtcaag agggaaacat aaagaaagtg agacactgac agaacaggat ttacagttat 1021 attgcgattt tcctaatatt attgatgtca gtattaagca agcaaaccaa gagggttcaa 1081 atgaaagccg agttgtaact atccataagc aagatggtaa aaatctggaa attgaactta 1141 gctcattaag ggaagctttg tctttcgtgt cattaattga tggatattat agattaactg 1201 cagatgcaca tcattacctc tgtaaagaag tagcacctcc agccgtgctt gaaaatatac 1261 aaagcaactg tcatggccca atttcgatgg attttgccat tagtaaactg aagaaagcag 1321 gtaatcagac tggactgtat gtacttcgat gcagtcctaa ggactttaat aaatattttt 1381 tgacttttgc tgtcgagcga gaaaatgtca ttgaatataa acactgtttg attacaaaaa 1441 atgagaatga agagtacaac ctcagtggga caaagaagaa cttcagcagt cttaaagatc 1501 ttttgaattg ttaccagatg gaaactgttc gctcagacaa tataattttc cagtttacta 1561 aatgctgtcc cccaaagcca aaagataaat caaaccttct agtcttcaga acgaatggtg 1621 tttctgatgt accaacctca ccaacattac agaggcctac tcatatgaac caaatggtgt 1681 ttcacaaaat cagaaatgaa gatttgatat ttaatgaaag ccttggccaa ggcactttta 1741 caaagatttt taaaggcgta cgaagagaag taggagacta cggtcaactg catgaaacag 1801 aagttctttt aaaagttctg gataaagcac acagaaacta ttcagagtct ttctttgaag 1861 cagcaagtat gatgagcaag ctttctcaca agcatttggt tttaaattat ggagtatgtg 1921 tctgtggaga cgagaatatt ctggttcagg agtttgtaaa atttggatca ctagatacat 1981 atctgaaaaa gaataaaaat tgtataaata tattatggaa acttgaagtt gctaaacagt 2041 tggcatgggc catgcatttt ctagaagaaa acacccttat tcatgggaat gtatgtgcca 2101 aaaatattct gcttatcaga gaagaagaca ggaagacagg aaatcctcct ttcatcaaac 2161 ttagtgatcc tggcattagt attacagttt tgccaaagga cattcttcag gagagaatac 2221 catgggtacc acctgaatgc attgaaaatc ctaaaaattt aaatttggca acagacaaat 2281 ggagttttgg taccactttg tgggaaatct gcagtggagg agataaacct ctaagtgctc 2341 tggattctca aagaaagcta caattttatg aagataggca tcagcttcct gcaccaaagt 2401 gggcagaatt agcaaacctt ataaataatt gtatggatta tgaaccagat ttcaggcctt 2461 ctttcagagc catcatacga gatcttaaca gtttgtttac tccagattat gaactattaa 2521 cagaaaatga catgttacca aatatgagga taggtgccct agggttttct ggtgcctttg 2581 aagaccggga tcctacacag tttgaagaga gacatttgaa atttctacag caacttggca 2641 agggtaattt tgggagtgtg gagatgtgcc ggtatgaccc tctacaggac aacactgggg 2701 aggtggtcgc tgtaaaaaag cttcagcata gtactgaaga gcacctaaga gactttgaaa 2761 gggaaattga aatcctgaaa tccctacagc atgacaacat tgtaaagtac aagggagtgt 2821 gctacagtgc tggtcggcgt aatctaaaat taattatgga atatttacca tatggaagtt 2881 tacgagacta tcttcaaaaa cataaagaac ggatagatca cataaaactt ctgcagtaca 2941 catctcagat atgcaagggt atggagtatc ttggtacaaa aaggtatatc cacagggatc 3001 tggcaacgag aaatatattg gtggagaacg agaacagagt taaaattgga gattttgggt 3061 taaccaaagt cttgccacaa gacaaagaat actataaagt aaaagaacct ggtgaaagtc 3121 ccatattctg gtatgctcca gaatcactga cagagagcaa gttttctgtg gcctcagatg 3181 tttGGAGCTT TGGAGTGGTT CTGTATGAAC TTTTCACATA CATTGAGAAG AGTAAAAGTC 3241 CACCAGCGGA ATTTATGCGC ATGATTGGCA ATGACAAACA AGGACAGATG ATCGTGTTCC 3301 ATTTGATAGA ACTTTTGAAG AATAATGGAA GATTACCAAG ACCAGATGGA TGCCCAGATG 3361 AGATCTATAT GATCATGACG GAATGCTGGA ACAATAATGT AAATCAACGC CCCTCCTTTA 3421 GGGATCTAGC TCTTCGAGTG GATCAAATAA GGGATAACAT GGCTGGATAG AACCCAGCTT 3481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3601 CTTGTGGAAA GGACGAAACG TGGACCCGTC CGTGGCAGGT ACGCGTTAAG TCgacaatca 3661 acctctggat tacaaaattt gtgaaagatt