Construct: ORF TRCN0000488229
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020644.1_s317c1
- DNA Barcode:
- GTCTAAAATAGCCAGCCTTCATGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RSKR (124923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488229
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124923 | RSKR | ribosomal protein S6 kinase... | NM_001174103.2 | 66.1% | 65.8% | 810_811insGGTGA;812_815delCCCC;822_1230del |
| 2 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532965.3 | 55.9% | 54.6% | (many diffs) |
| 3 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | NM_001287206.1 | 55.7% | 54.3% | (many diffs) |
| 4 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388406.3 | 55.3% | (many diffs) | |
| 5 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532966.3 | 51% | 49.2% | (many diffs) |
| 6 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388407.3 | 48.5% | (many diffs) | |
| 7 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532967.3 | 44.8% | 42.7% | (many diffs) |
| 8 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532968.3 | 32.1% | 33.7% | (many diffs) |
| 9 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532969.3 | 26.9% | 28.3% | (many diffs) |
| 10 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388408.3 | 20.5% | (many diffs) | |
| 11 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_001779956.1 | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 894
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggagca gtaagctgtc ggcaggggca gcacacccag cagggggaac 121 acacccgggt ggctgtccct cacaagcagg gtggcaacat ccggggtccc tgggcccgag 181 gctggaagag cctctggaca ggtttgggaa ccatcaggtc agatctggaa gaactctggg 241 aactacgggg gcaccactat ctgcaccagg aatccctaaa gccagcccca gtactggtag 301 agaagcctct gccagagtgg ccagtgcctc agttcatcaa cctctttcta ccagagtttc 361 ccattaggcc cattaggggg cagcagcagc tgaagatttt aggcctcgtg gctaaaggct 421 cctttggaac tgtcctcaag gtgctagatt gcacccagaa agctgtattt gcagtgaagg 481 tggtgcccaa ggtaaaggtc ctacagaggg ataccgtgag gcagtgcaaa gaggaggtta 541 gcatccagcg acagatcaac catccctttg tacacagctt ggggGACAGC TGGCAGGGAA 601 AACGGCACCT TTTCATTATG TGTAGCTACT GCAGCACAGA TCTGTACTCC CTTTGGTCGG 661 CTGTTGGCTG CTTTCCTGAG GCTTCCATCC GTCTCTTTGC TGCCGAGTTG GTGCTGGTAC 721 TGTGTTATCT CCATGACTTG GGCATCATGC ATCGAGATGT GAAGATGGAG AATATTCTTC 781 TAGATGAACG AGGCCATCTG AAACTGACAG ACTTTGGTCT GTCCCGCCAC GTGCCCCAGG 841 GAGCTCAAGC CTACACTATC TGTGGCACTC TTCAGTACAT GGGTGAGAGA GGTTAAGACC 901 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GAGTCTAAAA TAGCCAGCCT TCATGCACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt