Construct: ORF TRCN0000488235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019627.1_s317c1
- DNA Barcode:
- CGTGCCTCGTCCCGATTTAGCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR82 (27197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488235
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27197 | GPR82 | G protein-coupled receptor 82 | NM_080817.5 | 99.9% | 99.7% | 1008_1009insG |
| 2 | mouse | 319200 | Gpr82 | G protein-coupled receptor 82 | NM_175669.3 | 83.9% | 79.8% | (many diffs) |
| 3 | mouse | 319200 | Gpr82 | G protein-coupled receptor 82 | XM_011247475.1 | 83.9% | 79.8% | (many diffs) |
| 4 | mouse | 319200 | Gpr82 | G protein-coupled receptor 82 | XM_017318522.1 | 83.9% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1086
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaac aacaatacaa catgtattca accatctatg atctcttcca 121 tggctttacc aatcatttac atcctccttt gtattgttgg tgtttttgga aacactctct 181 ctcaatggat atttttaaca aaaataggta aaaaaacatc aacgcacatc tacctgtcac 241 accttgtgac tgcaaactta cttgtgtgca gtgccatgcc tttcatgagt atctatttcc 301 tgaaaggttt ccaatgggaa tatcaatctg ctcaatgcag agtggtcaat tttctgggaa 361 ctctatccat gcatgcaagt atgtttgtca gtctcttaat tttaagttgg attgccataa 421 gccgctatgc taccttaatg caaaaggatt cctcgcaaga gactacttca tgctatgaga 481 aaatatttta tggccattta ctgaaaaaat ttcgccagcc caactttgct agaaaactat 541 gcatttacat atggggagtt gtactgggca taatcattcc agttaccgta tactactcag 601 tcatagaggc tacagaagga gaagagagcc tatgctacaa tcggcagatg gaactaggag 661 ccatgatctc tcagattgca ggtctcattg gaaccacatt tattGGATTT TCCTTTTTAG 721 TAGTACTAAC ATCATACTAC TCTTTTGTAA GCCATCTGAG AAAAATAAGA ACCTGTACGT 781 CCATTATGGA GAAAGATTTG ACTTACAGTT CTGTGAAAAG ACATCTTTTG GTCATCCAGA 841 TTCTACTAAT AGTTTGCTTC CTTCCTTATA GTATTTTTAA ACCCATTTTT TATGTTCTAC 901 ACCAAAGAGA TAACTGTCAG CAATTGAATT ATTTAATAGA AACAAAAAAC ATTCTCACCT 961 GTCTTGCTTC GGCCAGAAGT AGCACAGACC CCATTATATT TCTTTTATTA GATAAAACAT 1021 TCAAGAAGAC ACTATATAAT CTCTTTACAA AGTCTAATTC AGCACATATG CAATCATATG 1081 GTGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGACG TGCCTCGTCC CGATTTAGCT ATACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt